Searched for: author%3A%22Lohman%2C+P.H.M.%22
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Mientjes, E.J. (author), Luiten-Schuite, A. (author), van der Wolf, E. (author), Borsboom, Y. (author), Bergmans, A. (author), Berends, F. (author), Lohman, P.H.M. (author), Baan, R.A. (author), van Delft, J.H.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
To investigate tissue-specific relations between DNA adducts and mutagenesis in vivo, λlacZ transgenic mice were treated i.p. with N-ethyl-N-nitrosourea (ENU), diethylnitrosamine (DEN), and ethyl methanesulphonate (EMS). In liver, bone marrow, and brain DNA from mice sacrificed at several time points after treatment O6-ethylguanine (O6-EtG) and...
article 1998
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Mientjes, E.J. (author), Hochleitner, K. (author), Luiten-Schuite, A. (author), van Delft, J.H.M. (author), Thomale, J. (author), Berends, F. (author), Rajewsky, M.F. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)
LacZ transgenic mice are suitable for short-term mutagenicity studies in vivo. Mutagenicity in these mice is determined in the lacZ transgene. Since the lacZ gene is of bacterial origin the question has been raised whether DNA-adduct formation and repair in the transgene are comparable to those in total genomic DNA. Mice were treated with N...
article 1996
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Mientjes, E.J. (author), Steenwinkel, M.J.S.T. (author), van Delft, J.H.M. (author), Lohman, P.H.M. (author), Baan, R.A. (author), TNO Voeding (author)
The recent introduction of the phenyl-β-D-galactopyranoside (P-gal)-based positive-selection system for screening of λlacZ phages originating from the λlacZ transgenic mouse (Muta Mouse) has made the determination of mutant frequencies (MF) a much simpler task. Previously, MF data from these mice have been collected by means of the 5-bromo-4...
article 1996
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TNO Preventie en gezondheid (author), Notten, W.R.F. (author), Berge, W.F.J.P.M. (author), Blaauboer, B.J. (author), Feron, V.J. (author), Lohman, P.H.M. (author), de Mik, G. (author), Woutersen, R.A. (author), van Zorge, J.A. (author), van Vliet, P.W. (author)
report 1996
document
Mientjes, E.J. (author), van Delft, J.H.M. (author), op 't Hof, B.M. (author), Gossen, J.A. (author), Vijg, J. (author), Lohman, P.H.M. (author), Baan, R.A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
article 1994
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Mol, M.A.E. (author), van der Schans, G.P. (author), Lohman, P.H.M. (author), Centraal Instituut voor Voedingsonderzoek TNO Medisch Biologisch Laboratorium TNO (author)
The induction of DNA interstrand cross-links and their repair has been studied in cultured human epidermal keratinocytes exposed to sulfur mustard, bis-(β-chloroethyl)sulfide. Alkaline elution is the most sensitive method to determine the number of DNA interstrand cross-links quantitatively. However, in the case of sulfur mustard the reliability...
article 1993
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Vink, A.A. (author), Berg, R.J.W. (author), de Gruijl, F.R. (author), Lohman, P.H.M. (author), Roza, L. (author), Baan, R.A. (author), Centraal Instituut voor Voedingsonderzoek TNO Medisch Biologisch Laboratorium TNO (author)
An immunocytochemical method was developed to study induction and removal of DNA damage in specific cell populations in the epidermis of hairless mice during chronic ultraviolet (UV) exposure. Identification of mouse suprabasal cells was performed with an immunoperoxidase stain. This stain was shown not to affect the fluorescent nuclear stains,...
article 1993
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van Loon, A.A.W.M. (author), Sonneveld, E. (author), Hoogerbrugge, J. (author), van der Schans, G.P. (author), Grootegoed, J.A. (author), Lohman, P.H.M. (author), Baan, R.A. (author), Centraal Instituut voor Voedingsonderzoek TNO Medisch Biologisch Laboratorium TNO (author)
article 1993
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van Loon, A.A.W.M. (author), Timmerman, A.J. (author), van der Schans, G.P. (author), Lohman, P.H.M. (author), Baan, R.A. (author), Medisch Biologisch Laboratorium TNO (author)
Exposure of cells to ionizing radiation gives rise to DNA damage, comprising strand breaks and base modifications. All these lesions may contribute to cell death, mutagenesis and/or carcinogenesis, but their relative contributions are likely to be different. It is important, therefore, to study the various damages with respect to their abundance...
article 1992
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Medisch Biologisch Laboratorium TNO (author), van Loon, A.A.W.M. (author), Groenendijk, R.H. (author), Timmerman, A.J. (author), van der Schans, G.P. (author), Lohman, P.H.M. (author), Baan, R.A. (author)
A simple, sensitive and fast immunochemical method has been developed to quantify the amount of DNA damage in cells of human blood after in vitro exposure to ionizing radiation. The technique is based on the enhancement of the radiation-induced single-strandedness, which occurs in DNA regions flanking strand breaks, by a controlled further...
article 1992
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Medisch Biologisch Laboratorium TNO (author), Roza, L. (author), Vermeulen, W. (author), Bergen Henegouwen, J.B.A. (author), Eker, A.P.M. (author), Jaspers, N.G.J. (author), Lohman, P.H.M. (author), Hoeijmakers, J.H.J. (author)
UV-induced thymine dimers (10 J/m2 of UV-C) were assayed in normal human and xeroderma pigmentosum (XP) fibroblasts with a monoclonal antibody against these dimers and quantitative fluorescence microscopy. In repair-proficient cells dimer-specific immunofluorescence gradually decreased with time, reaching about 25% of the initial fluorescence...
article 1990
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Medisch Biologisch Laboratorium TNO (author), Mullaart, E. (author), Lohman, P.H.M. (author), Berends, F. (author), Vijg, J. (author)
As a result of permanent exposure to low levels of various endogenous and exogenous genotoxic agents, large numbers of lesions are continuously induced in the DNA of cells of living organisms. Such lesions could lead to dysfunction of cells and tissues, and they might well be the underlying cause of the age-related reduction of homeostatic...
article 1990
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Medisch Biologisch Laboratorium TNO (author), Blok, J. (author), Aten, J.B.T. (author), Beekman, Z.M. (author), Beijerinck, D. (author), Bosnjakovic, B.F.M. (author), Davids, J.A.G. (author), Kal, H.B. (author), van Kempen, R.J. (author), Konings, A.W.T. (author), Lohman, P.H.M. (author), Pauwels, E.K.J. (author), Philippens, M.N.E.J.G. (author), Schaake-Koning, C.C.E. (author), Vos, O. (author), Passchier, W.F. (author), Schoten, E.J. (author)
report 1988
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Willems, M.I. (author), de Raat, W.K. (author), Baan, R.A. (author), Wilmer, J.W.G.M. (author), Lansbergen, M.J. (author), Lohman, P.H.M. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
The sensitivity of various methods suitable for biomonitoring the exposure to genotoxicants was compared in an animal model. The results were related to the presence of genotoxic effects in the target organ. Groups of male Wistar rats were given one oral dose of 0, 0.1, 1, 10 or 200 mg 2-acetylaminofluorene (2-AAF)/5 ml dimethyl sulphoxide/kg...
article 1987
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Lohman, P.H.M. (author), Berends, F. (author), Medisch Biologisch Laboratorium TNO (author)
bookPart 1986
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Medisch Biologisch Laboratorium TNO (author), Jansen, J.D. (author), Lohman, P.H.M. (author), Berends, F. (author)
article 1986
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Medisch Biologisch Laboratorium TNO (author), Jansen, J.D. (author), Lohman, P.H.M. (author), Berends, F. (author)
Deze literatuurstudie biedt een overzicht van methoden, die nu reeds voorhanden of in ontwikkeling zijn, om blootstelling aan DNA beschadigende stoffen bij werknemers op te kunnen sporen. De blootstelling aan mutagene of carcinogene stoffen wordt d.m.v. biological monitoring nagegaan. Development of methods for estimating exposure to mutagenic...
book 1985
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Centraal Instituut voor Voedingsonderzoek TNO Medisch Biologisch Laboratorium TNO (author), Baan, R.A. (author), Lansbergen, M.J. (author), de Bruin, P.A.F. (author), Willems, M.I. (author), Lohman, P.H.M. (author)
Exposure of cells to chemical carcinogens and mutagens may result in the formation of DNA adducts, which can give rise to mutations in the genome and to cellular transformation. Methods to measure DNA-adduct formation may be useful for 'biomonitoring', to establish exposure of laboratory animals or humans to DNA-damaging agents. For such...
article 1985
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Mohn, G.R. (author), Kerklaan, P.R.M. (author), van Zeeland, A.A. (author), Ellenberger, J. (author), Baan, R.A. (author), Lohman, P.H.M. (author), Pons, F.W. (author), Medisch Biologisch Laboratorium TNO (author)
article 1984
document
Lohman, P.H.M. (author), Instituut voor Landbouwkundig Onderzoek en Biochemische Produkten TNO (author)
conference paper 1984
Searched for: author%3A%22Lohman%2C+P.H.M.%22
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