Proteomics, an agnostic approach to detect emerging viruses - Poster -

The COVID-19 pandemic, but also MERS, SARS-1, monkeypox and other emerging biological threats have shown the pitfalls of diagnostics targeting only one or, at best, a limited number of pathogens. To overcome this, a next generation diagnostic platform that is causative-agent agnostic is developed. Moreover, the method is able to identify 1895...

Alcohol Consumption, Drinking Patterns, and Cognitive Performance in Young Adults: A Cross-Sectional and Longitudinal Analysis

Long-term alcohol abuse is associated with poorer cognitive performance. However, the associations between light and moderate drinking and cognitive performance are less clear. We assessed this association via cross-sectional and longitudinal analyses in a sample of 702 Dutch students. At baseline, alcohol consumption was assessed using...

In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota

Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli. Here, the gut microbiota-modulating effects of well-known and novel prebiotics were...

Clostridium botulinum group I strain genotyping by 15-locus multilocus variable-number tandem-repeat analysis

Clostridium botulinum is a taxonomic designation that encompasses a broad variety of spore-forming, Gram-positive bacteria producing the botulinum neurotoxin (BoNT). C. botulinum is the etiologic agent of botulism, a rare but severe neuroparalytic disease. Fine-resolution genetic characterization of C. botulinum isolates of any BoNT type is...

Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS

Background: The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desorption/ionization time...

Microarrays for genomotyping of pathogens

Microarrays for genomotyping of pathogens

Arginine-dependent acid resistance in Salmonella enterica serovar typhimurium

Salmonella enterica serovar Typhimurium does not survive a pH 2.5 acid challenge under conditions similar to those used for Escherichia coli (J. W. Foster, Nat. Rev. Microbiol. 2:898-907, 2004). Here, we provide evidence that S. enterica serovar Typhimurium can display arginine-dependent acid resistance (AM) provided the cells are grown under...

Survival, elongation, and elevated tolerance of Salmonella enterica serovar enteritidis at reduced water activity

Growing microorganisms on dry surfaces, which results in exposure to low water activity (aw), may change their normal morphology and physiological activity. In this study, the morphological changes and cell viability of Salmonella enterica serovar Enteritidis challenged to low a w were analyzed. The results indicated that exposure to reduced aw...