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Fluitman, K.S. (author), de Clercq, N.C. (author), Keijser, B.J.F. (author), Visser, M. (author), Nieuwdorp, M. (author), Ijzerman, R.G. (author)Introduction: Malnutrition refers to both over- and undernutrition and results from a disruption in energy balance. It affects one in three people worldwide and is associated with increased morbidity and mortality. The intestinal microbiota represents a newly identified factor that might contribute to the development of malnutrition, as it...article 2017
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Bosch, A.A.T.M. (author), de Steenhuijsen Piters, W.A.A. (author), van Houten, M.A. (author), Chu, M.L.J.N. (author), Biesbroek, G. (author), Kool, J. (author), Pernet, P. (author), de Groot, P.C.M. (author), Eijkemans, M.J.C. (author), Keijser, B.J.F.5. (author), Sanders, E.A.M. (author), Bogaert, D. (author)RATIONALE: Perinatal and postnatal influences are presumed important drivers of the early-life respiratory microbiota composition. We hypothesized that the respiratory microbiota composition and development in infancy is affecting microbiota stability and thereby resistance against respiratory tract infections (RTIs) over time. OBJECTIVES: To...article 2017
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Rosier, B.T. (author), van Loveren, C. (author), Zaura, E. (author), Loos, B.G. (author), Keijser, B.J.F. (author), Crielaard, W. (author), Lagerweij, M.D. (author)In the past, epidemiological studies focused on cavitated stages of caries. The arrival of the International Caries Detection and Assessment System (ICDAS) in 2004 allowed for clinical measurements of the initial stages of enamel caries. However, since the introduction, most studies applying the ICDAS still have studied the diseased population....article 2017
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- Imangaliyev, S. (author), van der Veen, M.H. (author), Volgenant, C.M.C. (author), Keijser, B.J.F. (author), Crielaard, W. (author), Levin, E. (author) conference paper 2016
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Rijkschroeff, P. (author), Jansen, I.D.C. (author), van der Weijden, F.A. (author), Keijser, B.J.F. (author), Loos, B.G. (author), Nicu, E.A. (author)Polymorphonuclear neutrophils (PMNs) have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs (oPMNs) compared with circulatory PMNs (cPMNs). Oral rinse and venous blood samples...article 2016
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Prodan, A. (author), Imangaliyev, S. (author), Brand, H.S. (author), Rosema, M.N.A. (author), Levin, E. (author), Crielaard, W. (author), Keijser, B.J.F. (author), Veerman, E.C.I. (author)Introduction: Understanding the changes occurring in the oral ecosystem during development of gingivitis could help improve prevention and treatment strategies for oral health. Erythritol is a non-caloric polyol proposed to have beneficial effects on oral health. Objectives: To examine the effect of experimental gingivitis and the effect of...article 2016
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Zaura, E. (author), Brandt, B.W. (author), de Mattos, M.J.T. (author), Buijs, M.J. (author), Caspers, M.P.M. (author), Rashid, M.U. (author), Weintraub, A. (author), Nord, C.E. (author), Savell, A. (author), Hu, Y. (author), Coates, A.R. (author), Hubank, M. (author), Spratt, D.A. (author), Wilson, M. (author), Keijser, B.J.F. (author), Crielaard, W. (author)Due to the spread of resistance, antibiotic exposure receives increasing attention. Ecological consequences for the different niches of individual microbiomes are, however, largely ignored. Here, we report the effects of widely used antibiotics (clindamycin, ciprofloxacin, amoxicillin, and minocycline) with different modes of action on the...article 2015
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Prodan, A. (author), Brand, H.S. (author), Ligtenberg, A.J.M. (author), Imangaliyev, S. (author), Tsivtsivadze, E. (author), van der Weijden, F. (author), Crielaard, W. (author), Keijser, B.J.F. (author), Veerman, E.C.I. (author)A cross-sectional observational study was conducted to evaluate interindividual biochemical variation in unstimulated whole saliva in a population of 268 systemically healthy young students, 18-30 yr of age, with no apparent caries lesions or periodontal disease. Salivary flow rate, protein content, pH, buffering capacity, mucins MUC5B and MUC7,...article 2015
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Janus, M.M. (author), Keijser, B.J.F. (author), Bikker, F.J. (author), Exterkate, R.A.M. (author), Crielaard, W. (author), Krom, B.P. (author)Commensal oral biofilms, defined by the absence of pathology-related phenotypes, are ubiquitously present. In contrast to pathological biofilms commensal biofilms are rarely studied. Here, the effect of the initial inoculum and subsequent growth conditions on in vitro oral biofilms was studied. Biofilms were inoculated with saliva and grown...article 2015
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Oliveira, S.C. (author), Slot, D.E. (author), Celeste, R.K. (author), Abegg, C. (author), Keijser, B.J.F. (author), van der Weijden, F.A. (author)Aim To evaluate the correlation between bleeding on marginal probing (BOMP) and bleeding on pocket probing (BOPP), and the correlation of both bleeding indices with plaque. Materials and Methods This cross-sectional study screened 336 participants, from which 268 were eligible for examination and analysis. Bleeding and plaque indices were...article 2015
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Rashid, M.U. (author), Zaura, E. (author), Buijs, M.J. (author), Keijser, B.J.F. (author), Crielaard, W. (author), Nord, C.E. (author), Weintraub, A. (author)The purpose of the study was to assess the effect of ciprofloxacin (500 mg twice daily for 10 days) or clindamycin (150 mg 4 times daily for 10 days) on the fecal microbiota of healthy humans for a period of 1 year as compared to placebo. Two different methods, culture and microbiome analysis, were used. Fecal samples were collected for analyses...article 2015
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Biesbroek, G. (author), Wang, X. (author), Keijser, B.J.F. (author), Eijkemans, R.M.J. (author), Trzciński, K. (author), Rots, N.Y. (author), Veenhoven, R.H. (author), Sanders, E.A.M. (author), Bogaert, D. (author)Seven-valent pneumococcal conjugate vaccine (PCV- 7) is effective against vaccine serotype disease and carriage. Nevertheless, shifts in colonization and disease toward nonvaccine serotypes and other potential pathogens have been described. To understand the extent of these shifts, we analyzed nasopharyngeal microbial profiles of 97 PCV-7...article 2014
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Biesbroek, G. (author), Tsivtsivadze, E. (author), Sanders, E.A.M. (author), Montijn, R. (author), Veenhoven, R.H. (author), Keijser, B.J.F. (author), Bogaert, D. (author)Rationale: Many bacterial pathogens causing respiratory infections in children are common residents of the respiratory tract. Insight into bacterial colonization patterns and microbiota stability at a young age might elucidate healthy or susceptible conditions for development of respiratory disease. Objectives: To study bacterial succession of...article 2014
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Biesbroek, G. (author), Bosch, A.A.T.M. (author), Wang, X. (author), Keijser, B.J.F. (author), Veenhoven, R.H. (author), Sanders, E.A.M. (author), Bogaert, D. (author)Rationale: Breastfeeding elicits significant protection against respiratory tract infections in infancy. Modulation of respiratory microbiota might be part of the natural mechanisms of protection against respiratory diseases induced by breastfeeding. Objectives: To study the association between breastfeeding and nasopharyngeal microbial...article 2014
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Imangaliyev, S. (author), Keijser, B.J.F. (author), Crielaard, W. (author), Tsivtsivadze, E. (author)As the amount of metagenomic data grows rapidly, online statistical learning algorithms are poised to play key rolein metagenome analysis tasks. Frequently, data are only partially labeled, namely dataset contains partial information about the problem of interest. This work presents an algorithm and a learning framework that is naturally...article 2013
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High-throughput analysis of the impact of antibiotics on the human intestinal microbiota compositionLadirat, S.E. (author), Schols, H.A. (author), Nauta, A. (author), Schoterman, M.H.C. (author), Keijser, B.J.F. (author), Montijn, R.C. (author), Gruppen, H. (author), Schuren, F.H.J. (author)Antibiotic treatments can lead to a disruption of the human microbiota. In this in-vitro study, the impact of antibiotics on adult intestinal microbiota was monitored in a new high-throughput approach: a fermentation screening-platform was coupled with a phylogenetic microarray analysis (Intestinal-chip). Fecal inoculum from healthy adults was...article 2013
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Özok, A.R. (author), Persoon, I.F. (author), Huse, S.M. (author), Keijser, B.J.F. (author), Wesselink, P.R. (author), Crielaard, W. (author), Zaura, E. (author)Aim To evaluate the microbial ecology of the coronal and apical segments of infected root canal systems using a complete sampling technique and next-generation sequencing. Methodology The roots of 23 extracted teeth with apical periodontitis were sectioned in half, horizontally, and cryo-pulverized. Bacterial communities were profiled usring...article 2012
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Kraneveld, E.A. (author), Buijs, M.J. (author), Bonder, M.J. (author), Visser, M. (author), Keijser, B.J.F. (author), Crielaard, W. (author), Zaura, E. (author)Currently there are no evidence-based ecological measures for prevention of overgrowth and subsequent infection by fungi in the oral cavity. The aim of this study was to increase our knowledge on fungal-bacterial ecological interactions. Salivary Candida abundance of 82 Dutch adults aged 58-80 years was established relative to the bacterial load...article 2012
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Biesbroek, G. (author), Sanders, E.A.M. (author), Roeselers, G. (author), Wang, X. (author), Caspers, M.P.M. (author), Trzciński, K. (author), Bogaert, D. (author), Keijser, B.J.F. (author)Introduction: Accurate analyses of microbiota composition of low-density communities (103-104 bacteria/sample) can be challenging. Background DNA from chemicals and consumables, extraction biases as well as differences in PCR efficiency can significantly interfere with microbiota assessment. This study was aiming to establish protocols for...article 2012
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Crielaard, W. (author), Zaura, E. (author), Schuller, A.A. (author), Huse, S.M. (author), Montijn, R.C. (author), Keijser, B.J.F. (author)BACKGROUND: An understanding of the relation of commensal microbiota to health is essential in preventing disease. Here we studied the oral microbial composition of children (N = 74, aged 3 - 18 years) in natural transition from their deciduous to a permanent dentition and related the microbial profiles to their oral health status. The microbial...article 2011