- document
-
Fluitman, K.S. (author), Wijdeveld, M. (author), Davids, M. (author), van Ruiten, C.C. (author), Reinders, I. (author), Wijnhoven, H.A.H. (author), Keijser, B.J.F. (author), Visser, M. (author), Nieuwdorp, M. (author), IJzerman, R.G. (author)Expert groups argue to raise the recommended daily allowance for protein in older adults from 0.8 to 1.2 g/kg/day to prevent undernutrition. However, protein is thought to increase satiety, possibly through effects on gut microbiota and central appetite regulation. If true, raising daily protein intake may work counterproductively. In a...article 2023
- document
-
Paauw, A. (author), Balvers, M. (author), de Koning, J.C. (author), Voskamp-Visser, A.I. (author), Keijser, B.J.F. (author), Kieboom, J. (author), Leeuwen, H.C. (author), Levin, E. (author)The COVID-19 pandemic, but also MERS, SARS-1, monkeypox and other emerging biological threats have shown the pitfalls of diagnostics targeting only one or, at best, a limited number of pathogens. To overcome this, a next generation diagnostic platform that is causative-agent agnostic is developed. Moreover, the method is able to identify 1895...conference paper 2022
- document
-
Fluitman, K.S. (author), Davids, M. (author), Olofsson, L.E. (author), Wijdeveld, M. (author), Tremaroli, V. (author), Keijser, B.J.F. (author), Visser, M. (author), Bäckhed, F. (author), Nieuwdorp, M. (author), IJzerman, R.G. (author)Background: Older adults are particularly prone to the development of poor appetite and undernutrition. Possibly, this is partly due to the aged gut microbiota. We aimed to evaluate the gut microbiota in relation to both poor appetite and undernutrition in community-dwelling older adults. Furthermore, we studied the causal effects of the...article 2022
- document
-
Kaan, A.M. (author), Brandt, B.W. (author), Buijs, M.J. (author), Crielaard, W. (author), Keijser, B.J.F. (author), Zaura, E. (author)In general, saliva is used for microbiota analysis in longitudinal studies, and several collection methods are being used. Using a robust sample collection procedure is important, as it may influence salivary composition. This study explored the comparability of the microbiota of swabbed and spit saliva. Twenty-two females participated in this...article 2022
- document
-
Rafiq, R. (author), Aleva, F.E. (author), Schrumpf, J.A. (author), Daniels, J.M. (author), Bet, P.M. (author), Boersma, W.G. (author), Bresser, P. (author), Spanbroek, M. (author), Lips, P. (author), van den Broek, T.J. (author), Keijser, B.J.F. (author), van der Ven, J.A.M. (author), Hiemstra, P.S. (author), den Heijer, M. (author), de Jongh, R.T. (author), PRECOVID-study group, (author)Background. Vitamin D deficiency is frequently found in patients with chronic obstructive pulmonary disease (COPD). Vitamin D has antimicrobial, anti-inflammatory, and immunomodulatory effects. Therefore, supplementation may prevent COPD exacerbations, particularly in deficient patients. Objectives. We aimed to assess the effect of vitamin D...article 2022
- document
-
Velders, A. (author), Ossendrijver, M. (author), Keijser, B.J.F. (author), Saggiomo, V. (author)Development of a novel LAMP device which is cheap, reusable, and can be produced in large amounts in a short period of time. The device was designed such not to require chemical exothermic reactions, have limited waste produced and with a minimum cost of the device as a whole. The device was tested for the detection of SARS-CoV2 RNA. Funding:...article 2021
- document
-
Fluitman, K.S. (author), Hesp, A.C. (author), Kaihatu, R.F. (author), Nieuwdorp, M. (author), Keijser, B.J.F. (author), IJzerman, R.G. (author), Visser, M. (author)BACKGROUND: Age-related declines in taste and smell function are widely assumed to contribute to the decrease in appetite and the development of undernutrition in older adults. OBJECTIVES: Here we aim to assess the associations of both taste and smell function with several nutrition-related outcomes in a single study, with poor appetite and...article 2021
- document
-
Steens, J.A. (author), Zhu, Y. (author), Taylor, D.W. (author), Bravo, J.P.K. (author), Prinsen, S.H.P. (author), Schoen, C.D. (author), Keijser, B.J.F. (author), Ossendrijver, M. (author), Hofstra, L.M. (author), Brouns, S.J.J. (author), Shinkai, A. (author), van der Oost, J. (author), Staals, R.H.J. (author)Characteristic properties of type III CRISPR-Cas systems include recognition of target RNA and the subsequent induction of a multifaceted immune response. This involves sequence-specific cleavage of the target RNA and production of cyclic oligoadenylate (cOA) molecules. Here we report that an exposed seed region at the 3′ end of the crRNA is...article 2021
- document
-
Fluitman, K.S. (author), van den Broek, T.J. (author), Nieuwdorp, M. (author), Visser, M. (author), IJzerman, R.G. (author), Keijser, B.J.F. (author)Poor taste and smell function are widely thought to contribute to the development of poor appetite and undernutrition in older adults. It has been hypothesized that the oral microbiota play a role as well, but evidence is scarce. In a cross-sectional cohort of 356 older adults, we performed taste and smell tests, collected anthropometric...article 2021
- document
-
Kiesswetter, E. (author), Keijser, B.J.F. (author), Volkert, D. (author), Visser, M. (author)Background: To prevent involuntary weight loss in older people, the knowledge about factors affecting body weight (BW) is essential. Therefore, we aimed to investigate the longitudinal associations of multiple oral health aspects with BW in community-dwelling older adults. Methods: This analysis is based on prospective data with a 10-year follow...article 2020
- document
-
Kaan, A.M. (author), Buijs, M.J. (author), Brandt, B.W. (author), Crielaard, W. (author), Keijser, B.J.F. (author), de Ruyter, J.C. (author), Zaura, E. (author)Objectives: Large longitudinal cohort studies in infants are needed to understand oral microbiome maturation in relation to general health. The logistics of such studies are complex and costs involved high. Methods like home sampling by caretakers might be a solution to these issues. This study aimed to evaluate feasibility of home sampling by...article 2020
- document
-
Rijkschroeff, P. (author), Schoenmaker, T. (author), Caspers, M. (author), Verschuren, L. (author), Keijser, B.J.F. (author), Nicu, E.A. (author), Loos, B.G. (author)Oral health and dentistry are essential components of systems medicine, which has received lesser attention in comparison to other medical fields, such as cancer biology. In this context, oral polymorphonuclear neutrophils (oPMNs) play an important role in the maintenance of oral health. To the best of our knowledge, this is the first study to...article 2020
- document
-
Fernandez-Gutierrez, M.M. (author), Imangaliyev, S. (author), Loos, B.G. (author), Keijser, B.J.F. (author), Kleerebezem, M. (author)Several proteins and peptides in saliva were shown to stimulate gingival wound repair, but the role of salivary metabolites in this process remains unexplored. In vitro gingival re-epithelialization kinetics were determined using unstimulated saliva samples from healthy individuals collected during an experimental gingivitis study. Elastic net...article 2020
- document
-
Man, W.H. (author), Clerc, M. (author), de Steenhuijsen Piters, W.A.A. (author), van Houten, M.A. (author), Chu, M.L.J.N. (author), Kool, J. (author), Keijser, B.J.F. (author), Sanders, E.A.M. (author), Bogaert, D. (author)Rationale: The respiratory microbiota is increasingly being appreciated as an important mediator in the susceptibility to childhood respiratory tract infections (RTIs). Pathogens are presumed to originate from the nasopharyngeal ecosystem. Objectives: To investigate the association between early life respiratory microbiota and development of...article 2019
- document
-
Keijser, B.J.F. (author), Agamennone, V. (author), van den Broek, T.J. (author), Caspers, M. (author), van de Braak, A. (author), Bomers, R. (author), Havekes, M. (author), Schoen, E. (author), van Baak, M. (author), Mioch, D. (author), Bomers, L. (author), Montijn, R.C. (author)Background: Antibiotic therapy is commonly used in animal agriculture. Antibiotics excreted by the animals can contaminate farming environments, resulting in long term exposure of animals to sub-inhibitory levels of antibiotics. Little is known on the effect of this exposure on antibiotic resistance. In this study, we aimed to investigate the...article 2019
- document
-
Fluitman, K.S. (author), Nadar, H.J. (author), Roos, D.S. (author), Berendse, H.W. (author), Keijser, B.J.F. (author), Nieuwdorp, M. (author), Ijzerman, R.G. (author), Visser, M. (author)Objectives: The olfactory decline that often accompanies aging is thought to contribute to undernutrition in older adults. It is believed to negatively affect eating pleasure, appetite, food intake and subsequently nutritional status. We have evaluated the associations of olfactory function with BMI, appetite and prospective weight change in a...article 2019
- document
-
van der Does, A.M. (author), Amatngalim, G.D. (author), Keijser, B.J.F. (author), Hiemstra, P.S. (author), Villenave, R. (author)The respiratory tract harbours a variety of microorganisms, collectively called the respiratory microbiota. Over the past few years, alterations in respiratory and gut microbiota composition have been associated with chronic inflammatory diseases of the lungs. How these changes influence disease development and progression is an active field of...article 2018
- document
-
Keijser, B.J.F. (author), van den Broek, T.J. (author), Slot, D.E. (author), van Twillert, L. (author), Kool, J. (author), Thabuis, C. (author), Ossendrijver, M. (author), van der Weijden, F.A. (author), Montijn, R.C. (author)Background: The oral cavity harbors a complex microbial ecosystem, intimately related to oral health and disease. The use of polyol-sweetened gum is believed to benefit oral health through stimulation of salivary flow and impacting oral pathogenic bacteria. Maltitol is often used as sweetener in food products. This study aimed to establish the...article 2018
- document
-
Arnoldussen, I.A.C. (author), Wiesmann, M. (author), Pelgrim, C.E. (author), Wielemaker, E.M. (author), van Duyvenvoorde, W. (author), Amaral-Santos, P.L. (author), Verschuren, L. (author), Keijser, B.J.F. (author), Heerschap, A. (author), Kleemann, R. (author), Wielinga, P.Y. (author), Kiliaan, A.J. (author)Objective: Midlife obesity affects cognition and increases risk of developing dementia. Recent data suggest that intake of the short chain fatty acid butyrate could improve memory function, and may protect against diet-induced obesity by reducing body weight and adiposity. Subjects: We examined the impact of a high-fat diet (HFD) followed by...article 2017
- document
-
Koopman, J.E. (author), Hoogenkamp, M.A. (author), Buijs, M.J. (author), Brandt, B.W. (author), Keijser, B.J.F. (author), Crielaard, W. (author), ten Cate, J.M. (author), Zaura, E. (author)Objective Bacterial metabolism of arginine in the oral cavity has a pH-raising and thus, potential anti-caries effect. However, the influence of arginine on the oral microbial ecosystem remains largely unresolved. Design In this pilot study, nine healthy individuals used toothpaste containing 8% arginine for eight weeks. Saliva was collected to...article 2017