Searched for: author%3A%22Jespersen%2C+S.%22
(1 - 20 of 20)
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Coulier, L. (author), Bas, R. (author), Jespersen, S. (author), Verheij, E. (author), van der Werf, M.J. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven TNO Voeding (author)
We have developed an analytical method, consisting of ion-pair liquid chromatography coupled to electrospray ionization mass spectrometry (IP-LC-ESI-MS), for the simultaneous quantitative analysis of several key classes of polar metabolites, like nucleotides, coenzyme A esters, sugar nucleotides, and sugar bisphosphates. The use of the ion-pair...
article 2006
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Verhoeckx, K.C.M. (author), Bijlsma, S. (author), Jespersen, S. (author), Ramaker, R. (author), Verheij, E.R. (author), Witkamp, R.F. (author), van der Greef, J. (author), Rodenburg, R.J.T. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
The discovery of new anti-inflammatory drugs is often based on an interaction with a specific target, although other pathways often play a primary or secondary role. Anti-inflammatory drugs can be categorized into classes, based on their mechanism of action. In this article we investigate the possibility to characterize novel anti-inflammatory...
article 2004
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Stierum, R. (author), Gaspari, M. (author), Dommels, Y. (author), Ouatas, T. (author), Pluk, H. (author), Jespersen, S. (author), Vogels, J. (author), Verhoeckx, K. (author), Groten, J. (author), van Ommen, B. (author)
Here, we describe a proteomics approach to study protein expression changes in differentiating Caco-2 cells. Caco-2 is a colorectal carcinoma cell line, which upon differentiation loses its tumorigenic phenotype and displays characteristics of mature enterocytes, including brush borders with microvilli. Cells were grown in culture flasks and...
article 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Loke, M.L. (author), Jespersen, S. (author), Vreeken, R. (author), Halling-Sørensen, B. (author), Tjørnelund, J. (author)
This paper describes the development of a HPLC-MS-MS (ESI) method with baseline separation of oxytetracycline, 4-epi-oxytetracycline, α-apo-oxytetracycline and β-apo-oxytetracycline using an XTerra column and an MeOH-MilliQ-water (containing 8 mM formic acid) mobile phase. Limits of quantification for aqueous standards were in the range of 0.004...
article 2003
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Chartogne, A. (author), Gaspari, M. (author), Jespersen, S. (author), Buscher, B. (author), Verheij, E. (author), van der Heijden, R. (author), Tjaden, U. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
In the present work, we describe a collection system for the off-line coupling of capillary isoelectric focusing (CIEF) with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. In this system, the capillary effluent is directly deposited in fractions onto the MALDI target via the use of a sheath liquid. The...
article 2002
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Jespersen, S. (author), Chaurand, P. (author), van Strien, F.J.C. (author), Spengler, B. (author), van der Greef, J. (author)
article 1999
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Vázquez-Martínez, R.M. (author), Malagón, M.M. (author), van Strien, F.J.C. (author), Jespersen, S. (author), van der Greef, J. (author), Roubos, E.W. (author), Gracia-Navarro, F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
We have previously shown that the melanotrope population of the pituitary intermediate lobe of Rana ridibunda is composed of two subpopulations, of low (LD) and high density (HD), that show distinct ultrastructural features and display different synthetic and secretory rates. To investigate whether LD and HD melanotrope cells also differ in...
article 1999
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Vázquez-Martínez, R.M. (author), Malagon, M.M. (author), van Strien, F.J.C. (author), Jespersen, S. (author), van der Greef, J. (author), Roubos, E.W. (author), Gracia-Navarro, F. (author)
article 1999
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Jespersen, S. (author), Chaurand, P. (author), van Strien, F.J.C. (author), Spengler, B. (author), van Greef, J.D. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Dissected tissue pieces of the pituitary pars intermedia from the amphibian Xenopus laevis was directly subjected to matrix-assisted laser desorption/ionization (MALDI) mass analysis. The obtained MALDI peptide profile revealed both previously known and unexpected processing products of the proopiomelanocortin gene. Mass spectrometric peptide...
article 1999
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Jespersen, S. (author)
doctoral thesis 1999
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Jespersen, S. (author), Niessen, W.M.A. (author), Tjaden, U.R. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A number of potential matrix candidates were investigated with regard to the importance of the pH in the matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) analysis of non-covalently bound protein complexes. The matrices examined were 2,5-dihydroxybenzoic acid (DHB), 4-hydroxy-α-cyanocinnamic acid (HCCA), 2-aminonicotinic...
article 1998
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Jespersen, S. (author), Ploemen, J.H.T.M. (author), Bladeren, P.J. (author), Niessen, W.M.A. (author), Tjaden, U.R. (author), van der Greef, J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
A mass spectrometric method providing qualitative site-specific information regarding covalent modification of proteins is described. The method involves comparison of unmodified and modified proteins by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) peptide mapping in combination with site-specific mutagenesis of...
article 1996
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Jespersen, S. (author), Niessen, W.M.A. (author), Tjaden, U.R. (author), van der Greef, J. (author), Litborn, E. (author), Lindberg, U. (author), Roeraade, J. (author), TNO Voeding (author)
article 1996
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Jespersen, S. (author), Koedam, J.A. (author), Hoogerbrugge, C.M. (author), Tjaden, U.R. (author), van der Greef, J. (author), van den Brande, J.L. (author), TNO Voeding (author)
High molecular weight precursors of insulin-like growth factor II (IGF-II) were isolated from Cohn fraction IV of human plasma by ultrafiltration, affinity chromatography and reversed-phase high-performance liquid chromatography. Molecular weight determination by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of two...
article 1996
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Rompelberg, C.J.M. (author), Ploemen, J.H.T.M. (author), Jespersen, S. (author), van der Greef, J. (author), Verhagen, H. (author), van Bladeren, P.J. (author), TNO Voeding (author)
The irreversible and reversible inhibition of glutathione S-transferases (GSTs) by eugenol was studied in rat, mouse and man. Using liver cytosol of human, rat and mouse, species differences were found in the rate of irreversible inhibition of GSTs by eugenol in the presence of the enzyme tyrosinase. Tyrosinase was used to oxidize eugenol. No...
article 1996
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), van Strien, F.J.C. (author), Jespersen, S. (author), van der Greef, J. (author), Jenks, B.G. (author), Roubos, E.W. (author)
The use of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in identifying proopiomelanocortin (POMC) processing products in melanotrope cells of the pituitary intermediate lobe of Xenopus laevis was explored. Mass spectra were obtained with such a high sensitivity of detection that the peptides could be identified in a...
article 1996
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Jespersen, S. (author), Niessen, W.M.A. (author), Tjaden, U.R. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
article 1995
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Centraal Instituut voor Voedingsonderzoek TNO (author), van Golen, F.A. (author), Wan Li, K. (author), Lange, R.P.J. (author), Jespersen, S. (author), Geraerts, W.P.M. (author)
article 1995
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Jespersen, S. (author), Niessen, W.M.A. (author), Tjaden, U.R. (author), van der Greef, J. (author), Litborn, E. (author), Lindberg, U. (author), Roeraade, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
article 1994
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Centraal Instituut voor Voedingsonderzoek TNO (author), Ploemen, J.H.T.M. (author), Johnson, W.W. (author), Jespersen, S. (author), Vanderwall, D. (author), van Ommen, B. (author), van der Greef, J. (author), van Bladeren, P.J. (author), Armstrong, R.N. (author)
article 1994
Searched for: author%3A%22Jespersen%2C+S.%22
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