Endotoxin-induced liver damage in rats is minimized by β2- adrenoceptor stimulation

Objective and Design: To investigate the effects of β2- adrenoceptor (β2-AR) stimulation on endotoxin-induced liver damage and systemic cytokine levels in rats. Subjects: Standard male Wistar rats. Treatment: A disease-model of lipolysaccharide (LPS)-induced acute systemic inflammation was used. The β2-selective AR agonist clenbuterol was...

The preparation, solubilisation and binding characteristics of a beta-2-adrenoceptor isolated from transfected Chinese hamster cells

Stereoselectivity at the B2-adrenoceptor on macrophages is a major determinant of the anti-inflammatory effects of B2-agonists

Previous research has shown that β-adrenoceptor (β-AR) agonists have potent anti-inflammatory capabilities, e.g. represented by suppression of release of the proinflammatory cytokines. Aim of this research was to determine whether the effects of β-agonists on LPS-induced TNFα and IL-10 release are influenced by their different stereochemistry....

Suppression of the acute inflammatory response of porcine alveolar- and liver macrophages

During infection and inflammation drug disposition and hepatic metabolism are markedly affected in mammals. Pro-inflammatory mediators play an important role in the suppression of (cytochrome-P450-mediated) drug metabolism. Inflammatory mediators like cytokines, nitric oxide (NO), reactive oxygen species (ROS) and eicosanoids are released by...

Development of a cell-based assay using flashplates for rapid detection of illegally used beta-agonists in bovine urine

Immunomodulatory properties of beta-adrenoceptor agonists: concepts for therapy of acute inflammatory disorders : Immuunmodulatie door beta-adrenoceptor agonisten : concepten voor de behandeling van acute ontstekingsreacties

The β-adrenoceptor agonist clenbuterol is a potent inhibitor of the LPS-induced production of TNF-α and IL-6 in vitro and in vivo

Objective and Design: To investigate the suppressive effects of the β-agonist clenbuterol on the release of TNF-α and IL-6 in a lipopolysaccharide (LPS)-model of inflammation, both in vitro and in vivo. Material and Subjects: Human U-937 cell line (monocyte-derived macrophages), and male Wistar rats (200-250 g). Treatment: U-937 macrophages were...

Participation of beta-adrenergic receptors on macrophages in modulation of lps-induces cytokine release

The beta-adrenoceptor agonist clenbuterol is a potent inhibitor of the LPS-inducedproduction of TNF-alpha and IL-6 in vitro and in vivo

Participation of β-adrenergic receptors on macrophages in modulation of LPS-induced cytokine release

For several years it is known that β-adrenergic receptor agonists have anti-inflammatory effects. However, little is known about the role of β- adrenergic receptors on macrophages in the modulation of cytokine production by β-agonists during inflammation. In this study, the presence of β- receptors on PMA-differentiated U937 human macrophages,...