document

Matrix degradation by chondrocytes cultured in alginate: IL-1β induces proteoglycan degradation and proMMP synthesis but does not result in collagen degradation

Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), TeKoppele, J.M. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)

Objective: To determine the role of interleukin-1β (IL-1β) in the degradation of proteoglycans and collagen by articular chondrocytes. Design: Chondrocytes were cultured in alginate beads for 2 weeks to produce extracellular matrix, followed by the addition of IL-1β for 1 or 2 days. Breakdown of extracellular matrix (with and without activation...

article 1998

document

Heterogeneous expression of cholesterol 7α-hydroxylase and sterol 27- hydroxylase genes in the rat liver lobulus

Twisk, J. (author), Hoekman, M.F.M. (author), Mager, W.H. (author), Moorman, A.F.M. (author), de Boer, P.A.J. (author), Scheja, L. (author), Princen, H.M.G. (author), Gebhardt, R. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)

We investigated the lobular localization and molecular level of expression of cholesterol 7α-hydroxylase and sterol 27-hydroxylase, two key enzymes in bile acid synthesis, in isolated periportal and pericentral hepatocytes and by in situ hybridization of rat liver. Enzyme activity, mRNA, and gene transcription of cholesterol 7α-hydroxylase were...

article 1995

document

Stimulation of tissue-type plasminogen activator gene expression by sodium butyrate and trichostatin A in human endothelial cells involves histone acetylation

Arts, J. (author), Lansink, T. (author), Grimbergen, J. (author), Toet, K.H. (author), Kooistra, T. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

We have previously shown that the pleiotropic agent sodium butyrate strongly stimulates tissue-type plasminogen activator (t-PA) expression in human umbilical vein endothelial cells (HUVEC). Here we provide the following evidence that the butyrate-induced t-PA expression in HUVEC involves histone H4 acetylation. (1) t-PA induction by butyrate...

article 1995

document

Clonal antibody dominance after influenza vaccination in IgA nephropathy patients and controls

Radl, J. (author), Hoogeveen, C.M. (author), van den Wall Bake, A.W.L. (author), Mestecky, J. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

Chemicals/CAS: hemagglutinin, 37333-12-3; sialidase, 9001-67-6; Antibodies, Monoclonal; Antibodies, Viral; Hemagglutinins, Viral; Immunoglobulin A; Immunoglobulin G; Influenza Vaccine; Neuraminidase, EC 3.2.1.18

article 1995

document

Comparison of the inhibition of urokinase-type plasminogen activator (u-PA) activity by monoclonal antibodies specific for u-PA as assessed by different assays

van Boheemen, P.A. (author), van den Hoogen, N.M. (author), Koolwijk, N. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)

Six murine monoclonal antibodies (MAbs) specific for urokinase-type plasminogen activator (u-PA) were tested for their ability to inhibit u-PA activity in three different assays with respect to amidolytic activity, plasminogen activation and fibrinolytic activity. Two of the MAbs were able to inhibit the amidolytic activity of u-PA. One MAb (LMW...

article 1995

document

Role of vitamin A in liver fibrosis

Knook, D.L. (author), Bosma, A. (author), Seifert, W.F. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)

The relationship between vitamin A and liver fibrosis was studied with a CCl4-induced fibrosis model in rats. Depending on the time of administration, vitamin A can potentiate or reduce fibrosis: when present during CCl4-treatment parenchymal cell damage and fibrosis were enhanced, whereas vitamin A post-treatment strongly reduced fibrosis....

article 1995

document

Prognostic relevance of plasminogen activators and their inhibitors in colorectal cancer

Ganesh, S. (author), Sier, C.F.M. (author), Griffioen, G. (author), Vloedgraven, H.J.M. (author), de Boer, A. (author), Welvaart, K. (author), van de Velde, C.J.H. (author), van Krieken, J.H.J.M. (author), Verheijen, J.H. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)

Human colorectal carcinogenesis has been shown previously to be associated with impressive changes in the tissue levels of plasminogen activators and their inhibitors, exemplified by an increase in the urokinase-type plasminogen activator (u-PA) and the inhibitors PAI-1 and PAI-2, and a decrease in tissue-type plasminogen activator (t-PA). In...

article 1994

document

Impaired fibrinolysis in the hemolytic-uremic syndrome of childhood

Menzel, D. (author), Levi, M. (author), Dooijewaard, G. (author), Peters, M. (author), ten Cate, J.W. (author), TNO Preventie en Gezondheid Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

article 1994

document

The fibrinolytic system in the hemolytic uremic syndrome: In vivo and in vitro studies

van de Kar, N.C.A.J. (author), van Hinsbergh, V.W.M. (author), Brommer, E.J.P. (author), Monnens, L.A.H. (author), Gaubius Laboratory TNO Preventie en Gezondheid Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

Fibrinolytic parameters and von Willebrand factor (vWF) antigen were measured in the plasma of 10 patients with hemolytic uremic syndrome (HUS). Samples were taken at presentation and again 2 wk later, before and after infusion of 1-desamino-8-arginine vasopressin. Compared with the plasma values of healthy control children, levels of tissue...

article 1994

document

Alcohol in combination with malnutrition causes increased liver fibrosis in rats

Bosma, A. (author), Seifert, W.F. (author), de Thiel van - Ruiter, G.C.F. (author), van Leeuwen, R.E.W. (author), Blauw, B. (author), Roholl, P. (author), Knook, D.L. (author), Brouwer, A. (author), Gaubius Laboratory TNO Preventie en Gezondheid Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

Rats were malnourished for 12 months with a highly inadequate fat-rich, calorie-sufficient but otherwise poly-deficient liquid diet composed of mashed potatoes with mayonnaise, comparable with the nutritional intake of many chronic alcoholics. When alcohol was incorporated into this diet, administered as whisky in drinking water available ad...

article 1994

document

Identification of two moieties of ß-endorphin with opposing effects on rat T-cell proliferation

van den Bergh, P. (author), Rozing, J. (author), Nagelkerken, L. (author), Gezondheidsorganisatie TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

In a previous study we demonstrated that beta-endorphin (beta-end) may stimulate rat T-cell proliferation via triggering of non-opioid receptors, whereas this stimulatory effect is abrogated by interaction of beta-end with opioid receptors. In the present study we provide evidence for this dualistic nature of beta-end by the identification of...

article 1993

document

Diverse effects of the Dutch guidelines for a healthy diet on serum lipids of male patients with type IIA and type IIB hyperlipoproteinemia

Wijsman, B.P. (author), Adrichem, C.M. (author), van 't Hooft, F.M. (author), Gevers Leuven, J.A. (author), van der Laarse, A. (author), Smelt, A.H.M. (author), Gaubius instituut Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

article 1993

document

Low-density lipoproteins are degraded in HepG2 cells with low efficiency

Lombardi, P. (author), Mulder, M. (author), de Wit, E. (author), van Berkel, T.J.C. (author), Frants, R.R. (author), Havekes, L.M. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

In previous studies we have shown that in HepG2 cells, as compared with fibroblasts, the low-density lipoprotein (LDL) receptor is only weakly down-regulated upon incubation of the cells with LDL. To explain this difference in down-regulation of the LDL-receptor activity, we studied simultaneously the intracellular processing of 125I-labelled...

article 1993

document

Lipoprotein (a): Relation to other risk factors and genetic heritability; results from a Dutch parent-twin study

Boomsma, D.I. (author), Kaptein, A. (author), Kempen, H.J. (author), Gevers Leuven, J.A. (author), Princen, H.M. (author), Gaubius instituut Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

We measured plasma levels of lipoprotein(a) (Lp(a)) in a sample of 152 Dutch adolescent mono- and dizygotic twin pairs and their parents. The distribution of Lp(a) levels was skewed, with the highest frequencies at low levels and was similar for adult men and women and their children. The relationship of Lp(a) concentrations with other...

article 1993

document

Experimental hypothyroidism increases plasminogen activator inhibitor activity in rat plasma

Padró, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

The aim of the present study was to investigate whether hypothyroidism would affect the components of the rat plasma fibrinolytic system. Hypothyroidism was induced by feeding rats a thiouracil-containing diet for 2 weeks. During this period, half of the animals received subcutaneous injections of L-thyroxine (20 μg/kg daily) to restore thyroid...

article 1993

document

Introduction. Minimally invasive therapy in five European countries: Diffusion, effectiveness and cost-effectiveness.

Banta, H.D. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

Minimally invasive therapy (MIT) is a field that has gained a great deal of attention since the mid-1980s. MIT is a new and rapidly growing area of medical treatment based on developments in endoscopy, vascular catheters, imaging devices, and related technologies. Although endoscopes have been in use for more than 100 years, it is relatively new...

article 1993

document

Molecular basis for fibrinogen Dusart (Aα 554 Arg → Cys) and its association with abnormal fibrin polymerization and thrombophilia

Koopman, J. (author), Haverkate, F. (author), Grimbergen, J. (author), Lord, S.T. (author), Mosesson, M.W. (author), DiOrio, J.P. (author), Siebenlist, K.S. (author), Legrand, C. (author), Soria, J. (author), Soria, C. (author), Caen, J.P. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

The molecular defect in the abnormal fibrinogen Dusart (Paris V) that is associated with thrombophilia was determined by sequence analysis of genomic DNA that had been amplified using the polymerase chain reaction. The propositus was heterozygous for a single base change (C → T) in the Aα- chain gene, resulting in the amino acid substitution Aα...

article 1993

document

Kwaliteitszorg radiodiagnostiek beheersbaar...?; een VZI studiedag

van Loon, A.J.P.M. (author), Centrum voor medische technologie TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

article 1993

document

The cost-effectiveness of 10 selected applications in minimally invasive therapy

Banta, H.D. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

In this article, evidence of effectiveness and cost-effectiveness of the following procedures is reviewed: (1) laser treatment of bladder tumors; (2) extracorporeal shock-wave lithotripsy and percutaneous nephrolithotomy; (3) laparoscopic treatment of endometriosis; (4) laparoscopic removal of ovarian cysts; (5) laparoscopic cholecystectomy; (6)...

article 1993

document

Evolutionary conservation of the mouse apolipoprotein e-c1-c2 gene cluster: Structure and genetic variability in inbred mice

Hoffer, M.J.V. (author), Hofker, M.H. (author), van Eck, M.M. (author), Havekes, L.M. (author), Frants, R.R. (author), Gaubius instituut Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

The human apolipoprotein E (APOE), APOC1, pseudo APOC1 (APOC1'), and APOC2 genes are clustered within 48 kb on the long arm of chromosome 19. A mouse Apoe cDNA probe was used to isolate overlapping cosmid clones from a cosmid library of the C57BL/Rij inbred mouse strain. These clones were investigated for the presence of the Apoc1 and Apoc2...

article 1993