Role of volatile fatty acids in development of the cecal microflora in broiler chickens during growth

It is known that volatile fatty acids can inhibit growth of species of the family Enterobacteriaceae in vitro. However, whether these volatile fatty acids affect bacterial populations in the ceca of chickens is unknown. Therefore, a study was conducted to investigate if changes in volatile fatty acids in ceca of broiler chickens during growth...

Development and application of in vitro intestinal tract model for safety evaluation of genetically modified foods

PCR-mediated methods for yeast typing and implementation in the food industry

DNA based typing, identification and detection systems for food spoilage microorganisms: Development and implementation

The rapid identification of spoilage microorganisms is of eminent importance to the food industry. It provides the food industry with the opportunity to reduce economical losses by designing adequate intervention measures. The use of identification systems based on biochemical and physiological characteristics resulted often in disappointing...

Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains

Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (GAC)5 and (GTG)5 enabled...

Identification of spoilage yeasts in a food-production chain by microsatellite polymerase chain reaction fingerprinting

A survey of yeast strains present in the production chain of mayonnaise and salad dressings was carried out over a period of 14 months. Attempts were made to identify the isolated yeasts with the API system, but identification of all species involved was not possible. In the investigation the performance of the microsatellite polymerase chain...

Investigation of an outbreak of Campylobacter upsaliensis in day care centers in Brussel: Analysis of relationships among isolates by phenotypic and genotypic typing methods

Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns

Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test

On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All...

Random amplified polymorphic DNA and restriction enzyme analysis of PCR amplified rDNA in taxonomy: Two identification techniques for food-borne yeasts

The random amplified polymorphic DNA (RAPD) assay and the restriction enzyme analysis of PCR amplified rDNA are compared for the identification of the common spoilage yeasts Zygosaccharomyces bailii, Z. rouxii, Saccharomyces cerevisiae, Candida valida and C. lipolytica. Both techniques proved to be adequate tools for yeast identification. Since...

Development of an ELISA and dot-blot assay to detect Mucor racemosus and related species of the order mucorales

RFLP analysis in chrysanthemum I: Probe and primer development

RAPD analysis: A rapid technique for differentation of spoilage yeasts

Techniques for the identification of the spoilage yeasts Saccharomyces cerevisiae and members of the Zygosaccharomyces genus from food and beverages sources were evaluated. The use of identification systems based on physiological characteristics resulted often in incomplete identification or misidentification. Also the cellular fatty acid...

Polymerase chain reaction for the specific detection of Escherichia coli/Shigella

The production and partial characterization of a monoclonal IgG antibody specific for moulds belonging to the order Mucorales

Ontwikkeling van DNA-probes voor de detectie van micro-organismen in de voedingsmiddelenmicrobiologie