Endotoxin-induced liver damage in rats is minimized by β2- adrenoceptor stimulation

Objective and Design: To investigate the effects of β2- adrenoceptor (β2-AR) stimulation on endotoxin-induced liver damage and systemic cytokine levels in rats. Subjects: Standard male Wistar rats. Treatment: A disease-model of lipolysaccharide (LPS)-induced acute systemic inflammation was used. The β2-selective AR agonist clenbuterol was...

Direct cell-to-cell contact between Kupffer cells and hepatocytes augments endotoxin-induced hepatic injury

This study focuses on the importance of direct contact between Kupffer cells (KCs) and hepatocytes (HCs) during the hepatic inflammatory response using an in vitro approach. The lipopolysaccharide (LPS)-induced inflammatory response in monocultures of porcine HCs and KCs were compared with cocultures prepared either with direct contact between...

Differential effects of pentoxifylline on the hepatic inflammatory response in porcine liver cell cultures increase in inducible nitric oxide synthase expression

Pentoxifylline (PTX) has been shown to exert hepatoprotective effects in various liver injury models. However, little information is available about the effect of PTX on the hepatic acute phase response. In the present study, the effect of PTX on a lipopolysaccharide (LPS)-induced acute phase response in primary porcine liver cell cultures was...

Cocultures of porcine hepatocytes and kupffer cells as an improved in vitro model for the study of hepatotoxic compounds