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Teunis, M.A.T. (author), Spiekstra, S.W. (author), Smits, M. (author), Adriaens, E. (author), Eltze, T. (author), Galbiati, V. (author), Krul, C.A.M. (author), Landsiedel, R. (author), Pieters, R. (author), Reinders, J. (author), Roggen, E. (author), Corsini, E. (author), Gibbs, S. (author)This study describes the international ring trial of the epidermal-equivalent (EE) sensitizer potency assay. This assay does not distinguish a sensitizer from a non-sensitizer, but may classify known skin sensitizers according to their potency. It assesses the chemical concentration resulting in 50% cytotoxicity (EE-EC50) or the 2-fold increase...article 2014
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Teunis, M. (author), Corsini, E. (author), Smits, M. (author), Madsen, C.B. (author), Eltze, T. (author), Ezendam, J. (author), Galbiati, V. (author), Gremmer, E. (author), Krul, C.A.M. (author), Landin, A. (author), Landsiedel, R. (author), Pieters, R. (author), Rasmussen, T.F. (author), Reinders, J. (author), Roggen, E. (author), Spiekstra, S. (author), Gibbs, S. (author)At present, the identification of potentially sensitizing chemicals is carried out using animal models. However, it is very important from ethical, safety and economic point of view to have biological markers to discriminate allergy and irritation events, and to be able to classify sensitizers according to their potency, without the use of...article 2013
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Gibbs, S. (author), van de Sandt, J.J.M. (author), Merk, H.F. (author), Lockley, D.J. (author), Pendlington, R.U. (author), Pease, C.K. (author), TNO Kwaliteit van Leven (author)In this review, we discuss and compare studies of xenobiotic metabolism in both human skin and 3D human skin reconstructs. In comparison to the liver, the skin is a less studied organ in terms of characterising metabolic capability. While the skin forms the major protective barrier to environmental chemical exposure, it is also a potential...article 2007
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Ponec, M. (author), Gibbs, S. (author), Pilgram, G. (author), Boelsma, E. (author), Koerten, H. (author), Bouwstra, J. (author), Mommaas, M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)One of the prerequisites for the use of human skin equivalents for scientific and screening purposes is that their barrier function is similar to that of native skin. Using human epidermis reconstructed on de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged...article 2001
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Ponec, M. (author), Gibbs, S. (author), GPilgram, G. (author), BoeIsma, E. (author), Koerten, H. (author), Bouwstra, J. (author), Mommaas, M. (author), TNO Voeding (author)One of the prerequisitesforthe use of human skin equivalents for scientific and screening purposes is that their barrierfunction is similar to that of native skin. Using human epidermis reconstructed en de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged...article 2001
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TNO Voeding (author), Ponec, M. (author), Gibbs, S. (author), Pilgram, G. (author), Boelsma, E. (author), Koerten, H. (author), Bouwstra, J. (author), Mommaas, M. (author)One of the prerequisites for the use of human skin equivalents for scientific and screening purposes is that their barrier function is similar to that of native skin. Using human epidermis reconstructed on de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged...article 2001