Searched for: author%3A%22Gaspari%2C+M.%22
(1 - 19 of 19)
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Blankestijn, M.A. (author), den Hartog Jager, C.F. (author), Blom, W.M. (author), Otten, H.G. (author), de Jong, G.A.H. (author), Gaspari, M. (author), Houben, G.F. (author), Knulst, A.C. (author), Verhoeckx, K.C.M. (author)
Background: The role of sensitization to commercially available allergens of English walnut (Juglans regia) Jug r 1, 2 and 3 in walnut allergy has been previously investigated in walnut allergic adults and was unable to explain all cases of walnut allergy. Objectives: Identify recognized walnut allergens, other than the ones previously...
article 2018
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Broekman, H.C.H.P. (author), Knulst, A.C. (author), de Jong, G. (author), Gaspari, M. (author), den Hartog Jager, C.F. (author), Houben, G.F. (author), Verhoeckx, K.C.M. (author)
Scope: The growing world population is a key driver for the exploration of sustainable protein sources to ensure food security. Mealworm and other insects are promising candidates. Previously we found that shrimp allergic patients are at risk for mealworm allergy, and that mealworm can induce a primary allergy. This study set out to investigate...
article 2017
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Broekman, H. (author), Knulst, A. (author), den Hartog Jager, S. (author), Monteleone, F. (author), Gaspari, M. (author), de Jong, G. (author), Houben, G. (author), Verhoeckx, K. (author)
Scope: The growing world population requires the exploration of new sustainable protein sources to ensure food security. Insects such as mealworm are promising candidates. For safety reasons, a risk assessment, including allergy risks, is needed. Since allergenicity can be influenced by thermal processing, it is highly important to take this...
article 2015
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Broekman, H. (author), Verhoeckx, K. (author), Gaspari, M. (author), den Hartog Jager, S. (author), Houben, G. (author), Knulst, A. (author)
Patients with a shrimp allergy, can also react to similar allergens from other crustaceans, shellfish or mollusks. Due to the quest for new protein sources, insects are being looked at to see if they could be a source for these proteins. In vitro studies show that the crustacean allergic patients are at risk to develop allergic symptoms when...
article 2014
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Verhoeckx, K.C.M. (author), van Broekhoven, S. (author), den Hartog-Jager, C.F. (author), Gaspari, M. (author), de Jong, G.A.H. (author), Wichers, H.J. (author), van Hoffen, E. (author), Houben, G.F. (author), Knulst, A.C. (author)
Scope: Due to the imminent growth of the world population, shortage of protein sources for human consumption will arise in the near future. Alternative and sustainable protein sources (e.g. insects) are being explored for the production of food and feed. In this project, the safety of Yellow mealworms (Tenebrio molitor L.) for human consumption...
article 2014
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Janda, E. (author), Palmieri, C. (author), Pisano, A. (author), Pontoriero, M. (author), Iaccino, E. (author), Falcone, C. (author), Fiume, G. (author), Gaspari, M. (author), Nevolo, M. (author), Di Salle, E. (author), Rossi, A. (author), de Laurentiis, A. (author), Greco, A. (author), Di Napoli, D. (author), Verheij, E. (author), Britti, D. (author), Lavecchia, L. (author), Quinto, I. (author), Scala, G. (author)
The inhibitor of Bruton tyrosine kinase γ (IBtkγ) is a negative regulator of the Bruton tyrosine kinase (Btk), which plays a major role in B-cell differentiation; however, the mechanisms of IBtkγ-mediated regulation of Btk are unknown. Here we report that B-cell receptor (BCR) triggering caused serine-phosphorylation of IBtkγ at protein kinase C...
article 2011
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Bansal, S. (author), Gaspari, M. (author), Raj, H.G. (author), Kumar, A. (author), Cuda, G. (author), Verheij, E. (author), Tyagi, Y.K. (author), Ponnan, P. (author), Rastogi, R.C. (author), Parmar, V.S. (author), TNO Kwaliteit van Leven (author)
Our earlier investigations identified acetoxy drug: protein transacetylase (TAase), a unique enzyme in the endoplasmic reticulum (ER) catalyzing the transfer of acetyl groups from polyphenolic acetates (PA) to certain functional proteins. Recently we have established the identity of TAase with ER protein calreticulin (CR) and subsequently...
article 2008
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Gaspari, M. (author), Verhoeckx, K.C.M. (author), Verheij, E.R. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)
LC-MS-based proteomics requires methods with high peak capacity and a high degree of automation, integrated with data-handling tools able to cope with the massive data produced and able to quantitatively compare them. This paper describes an off-line two-dimensional (2D) LC-MS method and its integration with software tools for data preprocessing...
article 2006
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Verhoeckx, K.C.M. (author), Gaspari, M. (author), Bijlsma, S. (author), van der Greef, J. (author), Witkamp, R.F. (author), Doornbos, R.P. (author), Rodenburg, R.J.T. (author), TNO Kwaliteit van Leven (author)
Two-dimensional difference gel electrophoresis (DIGE) in combination with univariate (Student's t-test) and multivariate data analysis, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to study the anti-inflammatory effects of the β2-adrenergic receptor (β2-AR) agonist zilpaterol. U937...
article 2005
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Koppelman, S.J. (author), Nieuwenhuizen, W.F. (author), Gaspari, M. (author), Knippels, L.M.J. (author), Penninks, A.H. (author), Knol, E.F. (author), Hefle, S.L. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
The high resistance of Brazil nut 2S albumin, previously identified as an allergen, against proteolysis by pepsin was examined in this work. Although the denaturation temperature of this protein exceeds the 110 °C at neutral pH, at low pH a fully reversible thermal denaturation was observed at ∼82 °C. The poor digestibility of the protein by...
article 2005
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Kohli, E. (author), Gaspari, M. (author), Raj, H.G. (author), Parmar, V.S. (author), Sharma, S.K. (author), van der Greef, J. (author), Kumari, R. (author), Gupta, G. (author), Khurana, P. (author), Tyagi, Y.K. (author), Watterson, A.C. (author), Olsen, C.E. (author), TNO Voeding (author)
The purification and characterization of the buffalo liver microsomal transacetylase (TAase) catalyzing the transfer of acetyl groups from a model acetoxy drug: 7,8-diacetoxy-4-methylcoumarin (DAMC) to GST3-3 has been described here. The enzyme was routinely assayed using DAMC and cytosolic GST as the substrates and was partially purified from...
article 2004
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Stierum, R. (author), Gaspari, M. (author), Dommels, Y. (author), Ouatas, T. (author), Pluk, H. (author), Jespersen, S. (author), Vogels, J. (author), Verhoeckx, K. (author), Groten, J. (author), van Ommen, B. (author)
Here, we describe a proteomics approach to study protein expression changes in differentiating Caco-2 cells. Caco-2 is a colorectal carcinoma cell line, which upon differentiation loses its tumorigenic phenotype and displays characteristics of mature enterocytes, including brush borders with microvilli. Cells were grown in culture flasks and...
article 2003
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Chartogne, A. (author), Gaspari, M. (author), Jespersen, S. (author), Buscher, B. (author), Verheij, E. (author), van der Heijden, R. (author), Tjaden, U. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
In the present work, we describe a collection system for the off-line coupling of capillary isoelectric focusing (CIEF) with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. In this system, the capillary effluent is directly deposited in fractions onto the MALDI target via the use of a sheath liquid. The...
article 2002
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Kohli, E. (author), Gaspari, M. (author), Raj, H.G. (author), Parmar, V.S. (author), van der Greef, J. (author), Gupta, G. (author), Kumari, R. (author), Prasad, A.K. (author), Goel, S. (author), Pal, G. (author), Tyagi, K. (author), Jain, S.C. (author), Ahmad, N. (author), Watterson, A.C. (author), Olsen, C.E. (author)
The current knowledge on biological protein acetylation is confined to acetyl CoA-dependent acetylation of protein catalyzed by specific acetyl transferases and the non-enzymatic acetylation of protein by acetylated xenobiotics such as aspirin. We have discovered a membrane-bound enzyme catalyzing the transfer of acetyl groups from the acetyl...
article 2002
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Centraal Instituut voor Voedingsonderzoek TNO (author), Kohli, E. (author), Gaspari, M. (author), Raj, H.G. (author), Parmar, V.S. (author), van der Greef, J. (author), Gupta, G. (author), Kumari, R. (author), Prasad, A.K. (author), Goel, S. (author), Pal, G. (author), Tyagi, Y.K. (author), Jain, S.C. (author), Ahmad, N. (author), Watterson, A.C. (author), Olsen, C.E. (author)
article 2002
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Gaspari, M. (author), Gucek, M. (author), Walhagen, K. (author), Vreeken, R.J. (author), Verheij, E.R. (author), Tjaden, U.R. (author), van der Greef, J. (author), TNO Voeding (author)
article 2001
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Walhagen, K. (author), Gaspari, M. (author), Tjaden, U.R. (author), Rozing, G.P. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)
A new in-house designed and constructed injection valve for capillary electrochromatography (CEC) based on a rotating injection part with compartments for the eluent as well as for the sample has been coupled to a mass spectrometer via a sheath flow electrospray ionisation (ESI) interface, using short capillary columns of 15 cm length. The CEC...
article 2001
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Tüdös, A.J. (author), Stigter, E.C.A. (author), van Hövel tot Westerflier, S.W.F.M. (author), van der Gaag, A. (author), Gaspari, M. (author)
article 2000
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Gucek, M. (author), Gaspari, M. (author), Walhagen, K. (author), Vreeken, R.J. (author), Verheij, E.R. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with...
article 2000
Searched for: author%3A%22Gaspari%2C+M.%22
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