Cyclopiazonic acid inhibits mutagenic action of aflatoxin B1

Cyclopiazonic acid and aflatoxin B1 are mycotoxins which can both be produced by the same moulds. Men can be exposed to these mycotoxins directly via ingestion of plant-derived food, as well as, indirectly via consumption of animal products. Although it is well known that aflatoxin B1 is mutagenic, contradictory results exist on the mutagenicity...

Characterization of biotransformation enzyme activities in primary rat proximal tubular cells

Direct cell-to-cell contact between Kupffer cells and hepatocytes augments endotoxin-induced hepatic injury

This study focuses on the importance of direct contact between Kupffer cells (KCs) and hepatocytes (HCs) during the hepatic inflammatory response using an in vitro approach. The lipopolysaccharide (LPS)-induced inflammatory response in monocultures of porcine HCs and KCs were compared with cocultures prepared either with direct contact between...

Mutagenicity of mercury chloride and mechanisms of cellular defence: The role of metal-binding proteins

Effect of fumonisin B1 on rat hepatic P450 system

The effects of the mycotoxin fumonisin B1 (FB1) on the hepatic cytochrome P450 system were investigated in male rats dosed daily by oral gavage with 3 mg FB1 per kg body weight for 9 consecutive days. FB1 treatment resulted in a reduced weight gain. At the same time, CYP2E activity was increased, which is considered to mark the metabolic changes...

Cocultures of porcine hepatocytes and kupffer cells as an improved in vitro model for the study of hepatotoxic compounds