Intestinal explant barrier chip: long-term intestinal absorption screening in a novel microphysiological system using tissue explants

The majority of intestinal in vitro screening models use cell lines that do not reflect the complexity of the human intestinal tract and hence often fail to accurately predict intestinal drug absorption. Tissue explants have intact intestinal architecture and cell type diversity, but show short viability in static conditions. Here, we present a...

Intestine-on-a-chip Next level in vitro research model of the human intestine

Over the past decade, microfluidic intestine-on-a-chip models have been emerging as a novel platform to study intestinal function in health and disease. These microphysiological systems surpass conventional in vitro intestinal model systems, as they add microenvironmental context in the form of mechanical cues or by the incorporation of multiple...

Microphysiological Systems to Recapitulate the Gut–Kidney Axis

Chronic kidney disease (CKD) typically appears alongside other comorbidities, highlighting an underlying complex pathophysiology that is thought to be vastly modulated by the bidirectional gut–kidney crosstalk. By combining advances in tissue engineering, biofabrication, microfluidics, and biosensors, microphysiological systems (MPSs) have...

A Theoretical and Experimental Study to Optimize Cell Differentiation in a Novel Intestinal Chip

Microphysiological systems have potential as test systems in studying the intestinal barrier, in which shear stress is critical for the differentiation of Caco-2 cells into enterocytes. The most commonly used in vitro gut model for intestinal barrier studies is based on trans-well cultures. Albeit useful, these culture systems lack physiological...

Monitoring the bio nanomechanical properties of cell structures

Organ-on-chip technology is based on tissues or (stem)cell derived organ mimicking structures and in microfluidic systems. This technology is used to monitor functional response to stimuli, aiming to enable personalized medicine and to conduct pre-clinical drug development studies. In general, cells in stress exhibit a change in nanomechanical...

Characterizing the invasion of different breast cancer cell lines with distinct E-cadherin status in 3D using a microfluidic system

E-cadherin is a cell-cell adhesion protein that plays a prominent role in cancer invasion. Inactivation of E-cadherin in breast cancer can arise from gene promoter hypermethylation or genetic mutation. Depending on their E-cadherin status, breast cancer cells adopt different morphologies with distinct invasion modes. The tumor microenvironment ...

Application of fresh human intestinal tissue in the inTESTine Chip: poster

The majority of screening and predictive models do not reflect the physiology of the human intestinal tract since they show major limitations to include the processes that determine the oral bioavailability1,2. A major drawback of current intestinal models is the use of single cell lines and the static environment, which is in contrast with the...