Gut-on-a-chip: accurate prediction of gut interventions: modelling the complex human gut

The Organ-on-a-Chip Early Research Program enables TNO to offer state-of-the-art technologies that help the pharmaceutical industry advance drug development. An accurate, viable alternative to animal testing, on-a-chip technologies model human tissue functioning and accelerate compound development and testing. The Intestinal Epithelial Barrier...

Pharmacokinetic application of normothermic perfused ex vivo porcine livers: poster

Current models to predict biliary excretion often fail due to species differences (rodent/dog) or due to differences in transporter expression in in vitro assays (e.g. sandwich cultured hepatocytes). Especially when drugs are subjective to enterohepatic circulation (EHC), this results difficulties to predict plasma profiles after oral and iv...

I-Screen: an ex vivo human microbiome platform to study microbiome induced reverse metabolism of metabolites back to parent: poster

Gut microbiota plays a major role in metabolizing xenobiotics into active, inactive or toxic metabolites, thereby influencing pharmacokinetics, efficacy and toxicity profiles of prescribed drugs, which is currently being underexplored.

Drug transporters: kinetics and metabolism

Transporter proteins are embedded in plasma membranes and actively transport their substrates (drugs, food components or endogenous compounds) into or out of the cell. Transporters play a significant role in the absorption, distribution and excretion of drugs. Drug-transporter interactions can cause unwanted drug-drug or drug-food interactions...

Quantitative protein abundance measurements: absolute quantification of plasma membrane measurements

Membrane embedded transporter proteins play an important role in the absorption, distribution and excretion of many drugs. A reliable prediction of human pharmacokinetics of (new) drugs based on PBPK modeling is highly dependent on accurate determination of the absolute protein expression levels of these transmembrane transporter proteins.