Mannosylated self-peptide inhibits the development of experimental autoimmune encephalomyelitis via expansion of nonencephalitogenic T cells

Tolerance to experimental autoimmune encephalomyelitis (EAE) in SJL mice can be induced by immunization with a mannosylated form of the proteolipid protein (M-PLP139-151), despite the presence of CFA. The state of tolerance is characterized by poor delayed-type hypersensitivity responses and the absence of clinical EAE symptoms. In vivo...

Immunization with mannosylated peptide induces poor T cell effector functions despite enhanced antigen presentation

In this study, we investigated the development of T cell responses in mice after administration of a mannosylated ovalbumin peptide (M-OVA323-339). Immunization with M-OVA323-339 in complete adjuvant resulted in enhanced antigen presentation in draining lymph nodes. Monitoring the fate of CFSE-labeled ovalbumin peptide-specific TCR transgenic...

Soluble mannosylated myelin peptide inhibits the encephalitogenicity of autoreactive T cells during experimental autoimmune encephalomyelitis

We have previously shown that immunization with a mannosylated myelin peptide in complete adjuvant induces tolerance instead of disease in experimental autoimmune encephalomyelitis (EAE), a rodent model for multiple sclerosis. In this report we demonstrate that treatment with a soluble mannosylated epitope of proteolipid protein (M-PLP139-151)...

Mannosylated PLP139-151 induces peptide-specific tolerance to experimental autoimmune encephalomyelitis

SJL mice immunized with mannosylated (M-) PLP139-151 in complete adjuvant do not develop EAE and little CNS mononuclear cell infiltration; other mannosylated peptides were ineffective in this experimental setting. Despite apparently normal T cell responses, M-PLP139-151-immunized mice show impaired delayed-type-sensitivity to PLP139-151 but a...

A novel and sensitive method for the detection of T cell stimulatory epitopes of α/β- and γ-gliadin

Background: It is now generally accepted that coeliac disease (CD) is caused by inflammatory T cell responses to gluten peptides bound to HLA-DQ2 or -DQ8 molecules. There is overwhelming evidence that CD patients can mount T cell responses to peptides found in both α-gliadin and γ-gliadin molecules. Assays that would detect the presence or...

Fluorogenic MMP activity assay for plasma including MMPs complexed to α2-macroglobulin

Elevated MMP activities are implicated in tissue degradation in, e.g., arthritis and cancer. The present study was designed to measure MMP enzyme activity in plasma. Free active MMP is unlikely to be present in plasma: upon entering the circulation, active MMP is expected to be captured by the proteinase inhibitor α2-macroglobulin (α2M)....

Fluorogenic activity assays for MMP-3 and MMP-I in synovial fluid.

Highly increased levels of active stromelysin in rheumatoid synovial fluid determined by a selective fluorogenic assay

Stromelysin-1 (MMP-3) is an important member of the matrix metalloproteinase family. In joint-degrading diseases like arthritis, elevated levels of MMP-3 protein are detected in synovial fluid using immunological methods. However, these methods do not discriminate between active and inactive enzyme. In the present study, a specific stromelysin...

Cleavage of collagen type I and II telopeptides by stromelysin is limited to the N-terminal telopeptide of collagen type II

Convenient fluorometric assay for matrix metalloproteinase activity and its application in biological media

Development of a convenient and sensitive assay for matrix metalloproteinase enzyme activity in synovial fluid samples using fluorigenic peptides