document

Molecular characterization of two lipoxygenases from barley

van Mechelen, J.R. (author), Schuurink, R.C. (author), Smits, M. (author), Graner, A. (author), Douma, A.C. (author), Sedee, N.J.A. (author), Schmitt, N.F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and...

article 1999

document

A convenient test for the determination of barley batch germination characteristics: Case studies in the malthouse

van Laarhoven, H.P.M. (author), van Iersel, M. (author), Dekkers, F. (author), Douma, A.C. (author)

conference paper 1999

document

Beer foam trouble shooting

Douma, A.C. (author), Bos, M.A. (author), Mocking-Bode, H.C.M. (author), Angelino, S.A.G.F. (author)

conference paper 1999

document

A novel optical method to determine oxygen in beer bottles

Draaijer, A. (author), König, J.W. (author), de Gans, O. (author), Jetten, J. (author), Douma, A.C. (author)

conference paper 1999

document

Flavour stability of pilsener beer

Angelino, S.A.G.F. (author), Kolkman, J.R. (author), van Gemert, L.J. (author), Vogels, J.T.W.E. (author), van Lonkhuijsen, H.J. (author), Douma, A.C. (author)

conference paper 1999

document

Evaluation of a malting barley quality assessment system

van Lonkhuijsen, H.J. (author), Douma, A.C. (author), Angelino, S.A.G.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

New malting barley varieties are annually tested for their malting and brewing potential according to a field trial set-up combined with quality evaluation on pilot scale. To assess the effects of trial year and location on quality evaluation data, a data base consisting of quality data from Dutch malting and brewing trials of new malting barley...

article 1998

document

Evaluation of a malting barley quality assessment system

van Lonkhuijsen, H.J. (author), Douma, A.C. (author), Angelino, S.A.G.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

article 1998

document

Structure-function relation of barley proteins in beer manufacturing

Angelino, S.A.G.F. (author), Douma, A.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

article 1998

document

Image analysis as practical tool in barley-malt-beer chains

van Laarhoven, H.P.M. (author), Douma, A.C. (author), Angelino, S.A.G.F. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)

conference paper 1997

document

Identification of foam-stabilizing proteins under conditons of normal beer dispense and their biochemical and physico-chemical properties

Douma, A.C. (author), Mocking-Bode, H.C.M. (author), Kooijman, M. (author), Stolzenbach, E. (author), Orsel, R. (author), Bekkers, A.C.A.P.A. (author), Angelino, S.A.G.F. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)

conference paper 1997

document

Expression and substrate specificity of lipoxygenase isoenzymes embryos of germinating barley

Holtman, W.L. (author), van Duijn, G. (author), van Mechelen, J.R. (author), Sedee, N.J.A. (author), Douma, A.C. (author), Schmitt, N. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)

bookPart 1997

document

Statistisch onderzoek naar de verbetering van de opzet van de gerstrassenbeproevingsprocedure m.b.t. de mout- en brouwkwaliteit

Centraal Instituut voor Voedingsonderzoek TNO (author), Douma, A.C. (author), Jansen, H. (author), Schoen, E.D. (author), Bonthuis, H. (author), van Lonkhuijsen, H.J. (author)

report 1997

document

Differential expression of lipoxygenase isoenzymes in embryos of germinating barley

Holtman, W.L. (author), van Duijn, G. (author), Sedee, N.J. (author), Sedee, N.J.A. (author), Douma, A.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

Expression of lipoxygenase was studied in barley (Hordeum distichum L.) embryos during germination. Total lipoxygenase activity was high in quiescent grains, dropped during the 1st d of germination, and subsequently increased to a level similar to that in quiescent grains. The contribution of two isoenzymes, lipoxygenases 1 (LOX-1) and 2 (LOX-2)...

article 1996

document

Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence

van Mechelen, J.R. (author), Smits, M. (author), Douma, A.C. (author), Rouster, J. (author), Cameron-Mills, V. (author), Heidekamp, F. (author), Valk, B.E. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A full length cDNA sequence for a barley grain lipoxygenase was obtained. It includes a 5' untranslated region of 69 nucleotides, an open reading frame of 2586 nucleotides encoding a protein of 862 amino acid residues and a 3' untranslated region of 142 nucleotides. The molecular mass of the encoded polypeptide was calculated to be 96.392. Its...

article 1995

document

Induction of lipase activity in germinating barley

Heistek, J.C. (author), Douma, A.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

conference paper 1995

document

Hydroperoxide dehydrase in barley grains

Schmitt, N. (author), Douma, A.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

conference paper 1995

document

Beta-oxidation of fatty acids is linked to the glyoxylate cycle in the aleurone but not in the embryo of germinating barley

Holtman, W.L. (author), Heistek, J.C. (author), Mattern, K.A. (author), Bakhuizen, R. (author), Douma, A.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

article 1994

document

The effect of external pH on the gibberellic acid response of barley aleurone

Sinjorgo, K.M.C. (author), de Vries, M.A. (author), Heistek, J.C. (author), van Zeijl, M.J. (author), van der Veen, S.W. (author), Douma, A.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

article 1993

document

Lipid degradation via beta-oxidation in germinating barley

Holtman, W.L. (author), Heistek, J.C. (author), Kokkelink, I. (author), Douma, A.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

conference paper 1993

document

Purification and characterization of two lipoxygenase isoenzymes from germinating barley

Center for Phytotechnology, RUL-TNO (author), Doderer, A. (author), Kokkelink, I. (author), van der Veen, S. (author), Valk, B.E. (author), Schram, A.W. (author), Douma, A.C. (author)

Two lipoxygenase isoenzymes (linoleate:oxygen oxidoreductase, EC 1.13.11.12) present in the embryo of germinating barley seed have been purified to homogeneity and characterized. Both isoenzymes are monomeric proteins with a molecular mass of approx. 90 kDa and crossreact on Western blots with antibodies raised against pea lipoxygenase. They...

article 1992