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Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A

Sarian, F.D. (author), van der Kaaij, R.M. (author), Kralj, S. (author), Wijbenga, D.-J. (author), Binnema, D.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)

Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little...

article 2012

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Structural characterization of bioengineered α-D-glucans produced by mutant glucansucrase GTF180 enzymes of lactobacillus reuteri strain 180

van Leeuwen, S.S. (author), Kralj, S. (author), Eeuwema, W. (author), Gerwig, G.J. (author), Dijkhuizen, L. (author), Kamerling, J.P. (author)

Mutagenesis of specific amino acid residues of the glucansucrase (GTF180) enzyme from Lactobacillus reuteri strain 180 yielded 12 mutant enzymes that produced modified exopolysaccharides (mEPSs) from sucrose. Ethanol-precipitated and purified mEPSs were subjected to linkage analysis, Smith degradation analysis, and 1D/2D ¹H NMR...

article 2009

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The unique branching patterns of DeinococcusGlycogen branching enzymes are determined by their N-Terminal domains

TNO Kwaliteit van Leven (author), Palomo, M. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)

article 2009

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Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles

Yuan, X.-L. (author), van der Kaaij, R.M. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven (author)

The filamentous ascomycete Aspergillus niger is well known for its ability to produce a large variety of enzymes for the degradation of plant polysaccharide material. A major carbon and energy source for this soil fungus is starch, which can be degraded by the concerted action of α-amylase, glucoamylase and α-glucosidase enzymes, members of the...

article 2008

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Exo-inulinase of Aspergillus niger N402: A hydrolytic enzyme with significant transfructosylating activity

Goosen, C. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)

The purified exo-inulinase enzyme of Aspergillus niger N402 (AngInuE; heterologously expressed in Escherichia coli) displayed a sucrose:inulin (S/I) hydrolysis ratio of 2.3, characteristic for a typical exo-inulinase. The enzyme also had significant transfructosylating activity with increasing sucrose concentrations, producing various...

article 2008

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Synthesis of branched polyglucans by the tandem action of potato phosphorylase and deinococcus geothermalis glycogen branching enzymes

van der Vlist, J. (author), Palomo Reixach, M. (author), van der Maarel, M. (author), Dijkhuizen, L. (author), Schouten, A.J. (author), Loos, K. (author), TNO Kwaliteit van Leven (author)

article 2008

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The probiotic Lactobacillus johnsonii NCC 533 produces high-molecular-mass inulin from sucrose by using an inulosucrase enzyme

TNO Kwaliteit van Leven (author), Anwar, M.A. (author), Kralj, S. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)

article 2008

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Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation/hydrolysis ratio

TNO Kwaliteit van Leven (author), Kralj, S. (author), van Leeuwen, S.S. (author), Valk, V. (author), Eeuwema, W. (author), Kamerling, J.P. (author), Dijkhuizen, L. (author)

The reuteransucrase enzymes of Lactobacillus reuteri strain 121 (GTFA) and L. reuteri strain ATCC 55730 (GTFO) convert sucrose into alpha-d-glucans (labelled reuterans) with mainly alpha-(1→4) glucosidic linkages (50% and 70%, respectively), plus alpha-(1→6) linkages. In the present study, we report a detailed analysis of various hybrid GTFA/O...

article 2008

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Molecular and biochemical characterization of a novel intracellular invertase from Aspergillus niger with transfructosylating activity

Goosen, C. (author), Yuan, X.L. (author), van Munster, J.M. (author), Ram, A.F.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)

A novel subfamily of putative intracellular invertase enzymes (glycoside hydrolase family 32) has previously been identified in fungal genomes. Here, we report phylogenetic, molecular, and biochemical characteristics of SucB, one of two novel intracellular invertases identified in Aspergillus niger. The sucB gene was expressed in Escherichia...

article 2007

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Identification of acceptor substrate binding subsites +2 +3 in the amylomaltase from Thermus thermophilus HB8

Kaper, T. (author), Leemhuis, H. (author), Uitdehaag, J.C.M. (author), van der Veen, B.A. (author), Dijkstra, B.W. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)

Glycoside hydrolase family 77 (GH77) belongs to the α-amylase superfamily (Clan H) together with GH13 and GH70. GH77 enzymes are amylomaltases or 4-α-glucanotransferases, involved in maltose metabolism in microorganisms and in starch biosynthesis in plants. Here we characterized the amylomaltase from the hyperthermophilic bacterium Thermus...

article 2007

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Two novel, putatively cell wall-associated and glycosylphosphatidylinositol-anchored α-glucanotransferase enzymes of Aspergillus niger

van der Kaaij, R.M. (author), Yuan, X.L. (author), Franken, A. (author), Ram, A.F.J. (author), Punt, P.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)

In the genome sequence of Aspergillus niger CBS 513.88, three genes were identified with high similarity to fungal α-amylases. The protein sequences derived from these genes were different in two ways from all described fungal α-amylases: they were predicted to be glycosylphosphatidylinositol anchored, and some highly conserved amino acids of...

article 2007

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Phylogenetic and biochemical characterization of a novel cluster of intracellular fungal α-amylase enzymes

van der Kaaij, R.M. (author), Janeček, Š. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)

Currently known fungal α-amylases are well-characterized extracellular enzymes that are classified into glycoside hydrolase subfamily GH13_1. This study describes the identification, and phylogenetic and biochemical analysis of novel intracellular fungal α-amylases. The phylogenetic analysis shows that they cluster in the recently identified...

article 2007

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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Pel, H.J. (author), de Winde, J.H. (author), Archer, D.B. (author), Dijkhuizen, L. (author), van den Hondel, C.A.M.J.J. (author), van der Kaaij, R.M. (author), van Kuyk, P.A. (author), van der Maarel, M.J.E. (author), TNO Kwaliteit van Leven (author)

article 2007

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Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-like transglycosylase

TNO Kwaliteit van Leven (author), Barends, T.R.M. (author), Bultema, J.B. (author), Kaper, T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), Dijkstra, B.W. (author)

Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book movie of the amylomaltase catalytic cycle in atomic detail. The structures include a covalent...

article 2007

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Database mining and transcriptional analysis of genes encoding inulin-modifying enzymes of Aspergillus niger

Yuan, X.L. (author), Goosen, C. (author), Kools, H. (author), van der Maarel, M.J.E.C. (author), van den Hondel, C.A.M.J.J. (author), Dijkhuizen, L. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven (author)

As a soil fungus, Aspergillus niger can metabolize a wide variety of carbon sources, employing sets of enzymes able to degrade plant-derived polysaccharides. In this study the genome sequence of A. niger strain CBS 513.88 was surveyed, to analyse the gene/enzyme network involved in utilization of the plant storage polymer inulin, and of sucrose,...

article 2006

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Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria

van Hijum, S.A.F.T. (author), Kralj, S. (author), Ozimek, L.K. (author), Dijkhuizen, L. (author), van Geel-Schutten, G.H. (author), TNO Kwaliteit van Leven (author)

Lactic acid bacteria (LAB) employ sucrase-type enzymes to convert sucrose into homopolysaccharides consisting of either glucosyl units (glucans) or fructosyl units (fructans). The enzymes involved are labeled glucansucrases (GS) and fructansucrases (FS), respectively. The available molecular, biochemical, and structural information on sucrase...

article 2006

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Single amino acid residue changes in subsite - 1 of inulosucrase from Lactobacillus reuteri 121 strongly influence the size of products synthesized

TNO Kwaliteit van Leven TNO Voeding (author), Ozimek, L.K. (author), Kralj, S. (author), Kaper, T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)

Bacterial fructansucrase enzymes belong to glycoside hydrolase family 68 and catalyze transglycosylation reactions with sucrose, resulting in the synthesis of fructooligosaccharides and/or a fructan polymer. Significant differences in fructansucrase enzyme product specificities can be observed, i.e. in the type of polymer (levan or inulin)...

article 2006

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Rational transformation of Lactobacillus reuteri 121 reuteransucrase into a dextransucrase

Kralj, S. (author), van Geel-Schutten, G.H. (author), Faber, E.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)

Glucansucrase or glucosyltransferase (GTF) enzymes of lactic acid bacteria display high sequence similarity but catalyze synthesis of different α-glucans (e.g., dextran, mutan, alternan, and reuteran) from sucrose. The variations in glucosidic linkage specificity observed in products of different glucansucrase enzymes appear to be based on...

article 2005

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Mutational analysis of the role of calcium ions in the Lactobacillus reuteri strain 121 fructosyltransferase (levansucrase and inulosucrase) enzymes

TNO Kwaliteit van Leven TNO Voeding (author), Ozimek, L.K. (author), Euverink, G.J.W. (author), Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)

Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D structure [Meng, G. and...

article 2005

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Amylomaltase of Pyrobaculum aerophilum IM2 produces thermoreversible starch gels

TNO Kwaliteit van Leven TNO Voeding (author), Kaper, T. (author), Talik, B. (author), Ettema, T.J. (author), Bos, H. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)

Amylomaltases are 4-alpha-glucanotransferases (EC 2.4.1.25) of glycoside hydrolase family 77 that transfer alpha-1,4-linked glucans to another acceptor, which can be the 4-OH group of an alpha-1,4-linked glucan or glucose. The amylomaltase-encoding gene (PAE1209) from the hyperthermophilic archaeon Pyrobaculum aerophilum IM2 was cloned and...

article 2005