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Wolken, W.A.M. (author), Tramper, J. (author), van der Werf, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)
Many organisms have the ability to form spores, a remarkable phase in their life cycles. Compared with vegetative cells, spores have several advantages (e.g. resistance to toxic compounds, temperature, desiccation and radiation) making them well suited to various applications. The applications of spores that first spring to mind are bio-warfare...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO (author), de Vocht, F. (author), van Wendel-de Joode, B. (author), Engels, H. (author), Kromhout, H. (author)
The interactive use of magnetic resonance imaging (MRI) techniques is increasing in operating theaters. A study was performed on 17 male company volunteers to assess the neurobehavioral effects of exposure to magnetic fields from a 1.5 Tesla MRI system. The subjects' neurobehavioral performances on a neurobehavioral test battery were compared in...
article 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Loke, M.L. (author), Jespersen, S. (author), Vreeken, R. (author), Halling-Sørensen, B. (author), Tjørnelund, J. (author)
This paper describes the development of a HPLC-MS-MS (ESI) method with baseline separation of oxytetracycline, 4-epi-oxytetracycline, α-apo-oxytetracycline and β-apo-oxytetracycline using an XTerra column and an MeOH-MilliQ-water (containing 8 mM formic acid) mobile phase. Limits of quantification for aqueous standards were in the range of 0.004...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO (author), Auffarth, J. (author), van Hemmen, J.J. (author), Hebisch, R. (author), Lechtenberg-Auffarth, E. (author), Marquart, J. (author), Oppl, R. (author), Rajan, B. (author), Wriedt, H. (author)
Reducing dermal exposure to chemicals is a challenging task for occupational safety and health. In the European RISKOFDERM project 15 institutes in ten member states gather dermal exposure information and measurement data in relation to various tasks. First results are available. Based on this information i) a predictive model for estimating...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO (author), Wijnands, M.V.W. (author)
doctoral thesis 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Korstanje, R. (author)
article 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), van Hijum, S.A.F.T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)
Inulosucrases catalyze transfer of a fructose moiety from sucrose to a water molecule (hydrolysis) or to an acceptor molecule (transferase), yielding inulin. Bacterial inulin production is rare and a biochemical analysis of inulosucrase enzymes has not been reported. Here we report biochemical characteristics of a purified recombinant...
article 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Yi, X. (author), Conesa, A. (author), Punt, P.J. (author), Hager, L.P. (author)
Site-directed mutagenesis has been used to investigate the role of glutamic acid 183 in chloroperoxidase catalysis. Based on the x-ray crystallographic structure of chloroperoxidase, Glu-183 is postulated to function on distal side of the heme prosthetic group as an acid-base catalyst in facilitating the reaction between the peroxidase and...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO (author), Sierksma, A. (author)
doctoral thesis 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), van Hemmen, J.J. (author)
article 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Kiely, M. (author), Faughnan, M. (author), Wähälä, K. (author), Brants, H. (author), Mulligan, A. (author)
The objective of the Vegetal Estrogens in Nutrition and the Skeleton (VENUS) project was to evaluate existing data on dietary exposure to compounds with oestrogenic and anti-oestrogenic effects present in plant foods as constituents or contaminants, and to identify and disseminate in vitro and in vivo methodologies to analyse the effects of such...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Blanquet, S. (author), Meunier, J.P. (author), Minekus, M. (author), Marol-Bonnin, S. (author), Alric, M. (author)
The use of genetically engineered microorganisms such as bacteria or yeasts as live vehicles to carry out bioconversion directly in the digestive environment is an important challenge for the development of innovative biodrugs. A system that mimics the human gastrointestinal tract was combined with a computer simulation to evaluate the survival...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Valtueña, S. (author), Cashman, K. (author), Robins, S.P. (author), Cassidy, A. (author), Kardinaal, A. (author), Branca, F. (author)
Research on the bone effects of natural phyto-oestrogens after menopause is at a relatively early stage. Published studies are few, difficult to compare and often inconclusive, due in part to design weaknesses. Currently, many questions remain to be answered including to what extent a safe daily intake may prevent postmenopausal bone loss. These...
article 2003
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Centraal Instituut voor Voedingsonderzoek TNO (author), van Erp-Baart, M.A.J. (author), Brants, H.A.M. (author), Kiely, M. (author), Mulligan, A. (author), Turrini, A. (author), Sermoneta, C. (author), Kilkkinen, A. (author), Valsta, L.M. (author)
The aim of this study was to identify the level of isoflavone intake (total isoflavones, daidzein and genistein) in four European countries: Ireland, Italy, The Netherlands and the UK. For this purpose national food composition databases of isoflavone content were created in a comparable way, using the Vegetal Estrogens in Nutrition and the...
article 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Rubingh, C.M. (author), Kruizinga, A.G. (author), Hulshof, K.F.A.M. (author), Brussaard, J.H. (author)
Probabilistic modelling can be used to get an insight into the variability and uncertainty of the nutrient intake in a population. When a probabilistic model is used, it is important that it is validated. Furthermore, a sensitivity analysis of the model output can give an insight into the most important input variables of the model and can be...
article 2003
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Chartogne, A. (author), Gaspari, M. (author), Jespersen, S. (author), Buscher, B. (author), Verheij, E. (author), van der Heijden, R. (author), Tjaden, U. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
In the present work, we describe a collection system for the off-line coupling of capillary isoelectric focusing (CIEF) with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. In this system, the capillary effluent is directly deposited in fractions onto the MALDI target via the use of a sheath liquid. The...
article 2002
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van Vliet, T. (author), Martin, A.H. (author), Bos, M.A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Recent studies on gelation and interfacial properties of vegetable protiens are reviewed. Attention is focused on legume proteins, mainly soy proteins, and on wheat proteins. The rheological properteis of vegetable protein gels as a function of heating time or temperature is discussed as well as the interfacial gelation upon adsorption of soy...
article 2002
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Mcewan, J.A. (author), Hunter, E.A. (author), van Gemert, L.J. (author), Lea, P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Proficiency testing in sensory analysis is an important step towards demonstrating that results from one sensory panel are consistent with the results of other sensory panels. The uniqueness of sensory analysis poses some specific problems for measuring the proficiency of the human instrument (panel). Individuals within a panel and people from...
article 2002
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Dunnewind, B. (author), van Vliet, T. (author), Orsel, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The use of enzymes such as peroxidases or glucose oxidase instead of chemical oxidants is a very interesting option for improving breadmaking performance of doughs. In this study the effect of such enzymes on bulk rheological properties of dough was quantified and their influence on the polymer network in dough deduced. Small deformation...
article 2002
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Hambly, R.J. (author), Saunders, M. (author), Rijken, P.J. (author), Rowland, I.R. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)
Although there is much epidemiological evidence for an interaction between diet and colorectal cancer risk, the mechanisms by which diet might protect against colorectal cancer are still unclear. Here we report the significant up-regulation of carcinogen-induced apoptosis in the colon of rats fed a diet containing low-risk factors for colon...
article 2002
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