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Verdoes, J.C. (author), Punt, P.J. (author), Burlingame, R. (author), Bartels, J. (author), van Dijk, R. (author), Slump, E. (author), Meens, M. (author), Joosten, R. (author), Emalfarb, M. (author), TNO Kwaliteit van Leven (author)
A self-replicating vector was designed that enables the construction of complex libraries in the fungus Chiysosporium lucknowense. The circular vector is linearized in vivo and results in a transformation frequency up to 13,000 transformants/ug ofplasmid DNA. Upon prolonged cultivation of the transformants in liquid medium, the linearized vector...
article 2007
Gusakov, A.V. (author), Sinitsyn, A.P. (author), Salanovich, T.N. (author), Bukhtojarov, F.E. (author), Markov, A.V. (author), Ustinov, B.B. (author), Zeijl, C.V. (author), Punt, P. (author), Burlingame, R. (author), TNO Kwaliteit van Leven (author)
Two forms of cellobiohydrolase I (CBH I, Cel7A) were purified from the culture ultrafiltrate of a mutant strain of the fungus Chrysosporium lucknowense, an industrial producer of cellulases and hemicellulases. The enzymes had different molecular masses (52 and 65 kDa, SDS-PAGE data) but the same pI (4.5). Peptide sequencing showed that a single...
article 2005