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Molhoek, E.M. (author), den Hertog, A.L. (author), de Vries, A.M.B.C. (author), Nazmi, K. (author), Veerman, E.C.I. (author), Hartgers, F.C. (author), Yazdanbakhsh, M. (author), Bikker, F.J. (author), van der Kleij, D. (author), TNO Defensie en Veiligheid (author)
Cathelicidins are effector molecules of the innate host defense system that establish an antimicrobial barrier at epithelial interfaces. The human cathelicidin LL-37, in addition to its antimicrobial activity, also exhibits immunomodulatory effects, such as inhibition of pro-inflammatory responses to bacterial LPS in human monocytic cells. In...
article 2009
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Lee, J. (author), Tomchick, D.R. (author), Brautigam, C.A. (author), Machius, M. (author), Kort, R. (author), Hellingwerf, K.J. (author), Gardner, K.H. (author), TNO Kwaliteit van Leven (author), KvL (author)
The Bacillus subtilis KinA protein is a histidine protein kinase that controls the commitment of this organism to sporulate in response to nutrient deprivation and several other conditions. Prior studies indicated that the N-terminal Per-ARNT-Sim domain (PAS-A) plays a critical role in the catalytic activity of this enzyme, as demonstrated by...
article 2008
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Weelink, S.A.B. (author), Tan, N.C.G. (author), ten Broeke, H. (author), van den Kieboom, C. (author), van Doesburg, W. (author), Langenhoff, A.A.M. (author), Gerritse, J. (author), Junca, H. (author), Stams, A.J.M. (author), TNO Bouw en Ondergrond (author)
A bacterium, strain BC, was isolated from a benzene-degrading chlorate-reducing enrichment culture. Strain BC degrades benzene in conjunction with chlorate reduction. Cells of strain BC are short rods that are 0.6 μm wide and 1 to 2 μm long, are motile, and stain gram negative. Strain BC grows on benzene and some other aromatic compounds with...
article 2008
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Kaper, T. (author), Leemhuis, H. (author), Uitdehaag, J.C.M. (author), van der Veen, B.A. (author), Dijkstra, B.W. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)
Glycoside hydrolase family 77 (GH77) belongs to the α-amylase superfamily (Clan H) together with GH13 and GH70. GH77 enzymes are amylomaltases or 4-α-glucanotransferases, involved in maltose metabolism in microorganisms and in starch biosynthesis in plants. Here we characterized the amylomaltase from the hyperthermophilic bacterium Thermus...
article 2007
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Weelink, S.A.B. (author), Tan, N.C.G. (author), ten Broeke, H. (author), van Doesburg, W. (author), Langenhoff, A.A.M. (author), Gerritse, J. (author), Stams, A.J.M. (author), TNO Bouw en Ondergrond (author)
A stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. The benzene degradation rate was 1.65 mM benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. Denaturing gradient gel electrophoresis of...
article 2007
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Egert, M. (author), de Graaf, A.A. (author), Maathuis, A. (author), de Waard, P. (author), Plugge, C.M. (author), Smidt, H. (author), Deutz, N.E.P. (author), Dijkema, C. (author), de Vos, W.M. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)
16S rRNA-based stable isotope probing (SIP) and nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling were used to identify bacteria fermenting glucose under conditions simulating the human intestine. The TIM-2 in vitro model of the human intestine was inoculated with a GI tract microbiota resembling that of the small intestine...
article 2007
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van Hijum, S.A.F.T. (author), Kralj, S. (author), Ozimek, L.K. (author), Dijkhuizen, L. (author), van Geel-Schutten, G.H. (author), TNO Kwaliteit van Leven (author)
Lactic acid bacteria (LAB) employ sucrase-type enzymes to convert sucrose into homopolysaccharides consisting of either glucosyl units (glucans) or fructosyl units (fructans). The enzymes involved are labeled glucansucrases (GS) and fructansucrases (FS), respectively. The available molecular, biochemical, and structural information on sucrase...
article 2006
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Levantesi, C. (author), Rossetti, S. (author), Thelen, K. (author), Kragelund, C. (author), Krooneman, J. (author), Eikelboom, D. (author), Nielsen, P.H. (author), Tandoi, V. (author)
Twelve strains of filamentous bacteria morphologically identified as 'Microthrix parvicella' were isolated from industrial activated sludge wastewater treatment plants. 16S rRNA gene sequences analysis showed that these strains were all closely related to 'Candidatus Microthrix parvicella'. Six of them, however, had a 16S rRNA gene similarity of...
article 2006
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van Hijum, S.A.F.T. (author), Szalowska, E. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Lactobacillus reuteri strain 121 employs a fructosyltransferase (FTF) to synthesize a fructose polymer [a fructan of the levan type, with β(2→6) linkages] from sucrose or raffinose. Purification of this FTF (a levansucrase), and identification of peptide amino acid sequences, allowed isolation of the first Lactobacillus levansucrase gene (lev),...
article 2004
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Majhenič, A.Č. (author), Venema, K. (author), Allison, G.E. (author), Matijašić, B.B. (author), Rogelj, I. (author), Klaenhammer, T.R. (author), TNO Voeding (author)
Lactobacillus gasseri LF221, an isolate from the feces of a child, produces two bacteriocins. Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA carrying the acidocin LF221 A and B structural genes, respectively. Sequencing analysis revealed the gene of acidocin LF221 A to...
article 2004
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van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)
In a follow-up of the earlier characterisation of botulinum toxins type A and B (BTxA and BTxB) by mass spectrometry (MS), types C, D, E, and F (BTxC, BTxD, BTxE, BTxF) were now investigated. Botulinum toxins are extremely neurotoxic bacterial toxins, likely to be used as biological warfare agent. Biologically active BTxC, BTxD, BTxE, and BTxF...
article 2004
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Ozimek, L.K. (author), van Hijum, S.A.F.T. (author), van Koningsveld, G.A. (author), van der Maarel, M.J.E.C. (author), van Geel-Schutten, G.H. (author), Dijkhuizen, L. (author)
Bacterial fructosyltransferases (FTFs) are retaining-type glycosidases that belong to family 68 of glycoside hydrolases. Recently, the high-resolution 3D structure of the Bacillus subtilis levansucrase has been solved [Meng, G. and Futterer, K., Nat. Struct. Biol. 10 (2003) 935-941]. Based on this structure, the catalytic nucleophile, general...
article 2004
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Arand, M. (author), Hallberg, B.M. (author), Zou, J. (author), Bergfors, T. (author), Oesch, F. (author), van der Werf, M.J. (author), de Bont, J.A.M. (author), Jones, T.A. (author), Mowbray, S.L. (author)
Epoxide hydrolases are essential for the processing of epoxide-containing compounds in detoxification or metabolism. The classic epoxide hydrolases have an α/β hydrolase fold and act via a two-step reaction mechanism including an enzyme-substrate intermediate. We report here the structure of the limonene-1,2-epoxide hydrolase from Rhodococcus...
article 2003
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Berry, A. (author), Janssens, D. (author), Hümbelin, M. (author), Jore, J.P.M. (author), Hoste, B. (author), Cleenwerck, I. (author), Vancanneyt, M. (author), Bretzel, W. (author), Mayer, A.F. (author), Lopez-Ulibarri, R. (author), Shanmugam, B. (author), Swings, J. (author), Pasamontes, L. (author)
A comprehensive taxonomic re-evaluation was performed on the marine, zeaxanthin-producing bacterium formerly classified as [Flavobacterium] sp. strain R-1512 (ATCC 21588). This strain, together with two other previously described marine isolates, [Flavobacterium] strain R-1506 and Paracoccus sp. strain MBIC 3966, were found to comprise a new...
article 2003
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Prins Maurits Laboratorium TNO (author), Broekhuijsen, M.P. (author), Larsson, P. (author), Johansson, A. (author), Byström, M. (author), Eriksson, U. (author), Larsson, E. (author), Prior, R.G. (author), Sjöstedt, A. (author), Titball, R.W. (author), Forsman, M. (author)
Francisella tularensis is a potent pathogen and a possible bioterrorism agent. Little is known, however, to explain the molecular basis for its virulence and the distinct differences in virulence found between the four recognized subspecies, F. tularensis subsp. tularensis, F. tularensis subsp. mediasiatica, F. tularensis subsp. holarctica, and...
article 2003
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van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)
A method earlier developed for the mass spectrometric (MS) identification of tetanus toxin (TTx) was applied to botulinum toxins type A and B (BTxA and BTxB). Botulinum toxins are extremely neurotoxic bacterial toxins, likely to be used as biological warfare agent. Biologically active BTxA and BTxB are comprised of a protein complex of the...
article 2002
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Smit, E. (author), Jager, D. (author), Martinez, B. (author), Tielen, F.J. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The structure of the crystallisation domain, SAN, of the S A-protein of Lactobacillus acidophilus ATCC 4356 was analysed by insertion and deletion mutagenesis, and by proteolytic treatment. Mutant S A-protein synthesised in Escherichia coli with 7-13 amino acid insertions near the N terminus or within regions of sequence variation in SAN (amino...
article 2002
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Posthuma, C.C. (author), Bader, R. (author), Engelmann, R. (author), Postma, P.W. (author), Hengstenberg, W. (author), Pouwels, P.H. (author)
Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described, xpkA encodes a 788-amino-acid protein with a calculated mass of 88,705 Da. Expression of xpkA...
article 2002
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Vriesema, A.J.M. (author), Dankert, J. (author), Zaat, S.A.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
We aimed to identify transcription signal sequences from Streptococcus gordonii strain CH1 by random chromosomal cloning. Five genomic fragments from a Sau3A digest, which constitutively activated transcription of a promoterless spectinomycin resistance gene in this strain, were isolated and characterized. Additionally, one promoter fragment was...
article 1999
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van Baar, B.L.M. (author), Hulst, A.G. (author), Wils, E.R.J. (author), TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA, Rijswijk, Netherlands (author)
Cholera toxin, one of the toxins that may be generated by various strains of the bacterium Vibrio cholerae, can be considered as a substance possibly used in biological warfare. The possibilities of characterising the toxin by liquid chromatography electrospray mass spectrometry (LC-ES-MS) were investigated. The toxin can be detected by flow...
article 1999
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