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Bosma, P.J. (author), van den Berg, E.A. (author), Kooistra, T. (author), Siemieniak, D.R. (author), Slightom, J.L. (author), Gaubius Institute TNO, 2313 AD Leiden, Netherlands (author)
We have determined the nucleotide sequence of the human plasminogen activator inhibitor-1 (PAI-1) gene and significant stretches of DNA which extend into its 5'- and 3'-flanking DNA regions; a total sequence of 15,867 base pairs (bp) is presented. The sequenced 5'-flanking DNA (1,520 bp) contains the essential eukaryotic cis-type proximal...
article 1988
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Gaubius Instituut TNO (author), Rijken, D.C. (author), Groeneveld, E. (author), Kluft, C. (author), Nieuwenhuis, H.K. (author)
α2-Antiplasmin Enschede is a variant of α2-antiplasmin which has lost its ability to inhibit plasmin irreversibly and which is associated with haemorrhagic disorder [Kluft et al. (1987) J. Clin. Invest. 80, 1391-1400]. The abnormal protein was purified from the plasma of a homozygous patient and subjected to one-dimensional peptide mapping using...
article 1988
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van Noort, J.M. (author), van der Drift, A.C.M. (author), Medisch Biologisch Laboratorium TNO (author)
The selectivity of cathepsin D, a mammalian intracellular aspartyl proteinase involved in the degradation of endocytosed proteins, was studied. For this purpose, several proteins of known primary structure were subjected to mild proteolysis by the enzyme, and the preferentially cleaved peptide bonds were identified. Comparison of the primary...
article 1989
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Weening-Verhoeff, E.J.D. (author), Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Rehberg, E.F. (author), Marotti, K.R. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
article 1990
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Schielen, W.J. (author), Voskuilen, M. (author), Adams, P.J. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)
Fibrin, not fibrinogen, enhances the rate of tPA catalysed plasminogen activation. In earlier studies we have shown that a site involved in this rate enhancement is located in a tridecapeptide, i.e. fibrinogen A alpha-(148-160). This sequence comprises a special charge distribution in which a stretch with alternating neutral and acidic amino...
article 1990
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Burger, H. (author), van Leen, R.W. (author), Dorssers, L.C.J. (author), Persoon, N.L.M. (author), Lemson, P.J. (author), Wagemaker, G. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)
To enable preclinical studies on homologous interleukin-3 (IL-3) in primate species, we isolated the gene encoding Rhesus monkey IL-3 (RhIL-3). The nucleotide sequence of the RhIL-3 gene displayed 92.9% homology with that of the human IL-3 (hIL-3) gene. The isolated RhIL-3 gene encodes a 143-amino acid (aa) precursor polypeptide, nine C-terminal...
article 1990
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Lokman, B.C. (author), van Santen, P. (author), Verdoes, J.C. (author), Kruse, J. (author), Leer, R.J. (author), Posno, M. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)
A cluster of three genes involved in D-xylose catabolism (viz. xylose genes) in Lactobacillus pentosus has been cloned in Escherichia coli and characterized by nucleotide sequence analysis. The deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in Bacillus...
article 1991
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Koopman, J. (author), Haverkate, F. (author), Briet, E. (author), Lord, S.T. (author), Gaubius Institute TNO (author)
A congenitally abnormal fibrinogen (Vlissingen) was isolated from the blood of a young woman suffering from massive pulmonary embolism. Fibrinogen Vlissingen showed an abnormal clotting time with both thrombin and Reptilase(TM). The release of the fibrino-peptides A and B by thrombin was normal, but fibrin polymerization was impaired both in the...
article 1991
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Schielen, W.J. (author), Adams, H.P. (author), Voskuilen, M. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
The rate of activation of plasminogen by tissue-type plasminogen activator (t-PA) is greatly increased by fibrin, but much less by fibrinogen. Fibrin(ogen) fragments such as the fibrin(ogen) cyanogen bromide fragment FCB-2 and FCB-5, and a synthetic peptide with the sequence of fibrinogen A alpha-(148-160), a constituent of FCB-2, also have rate...
article 1991
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Schielen, W.J.G. (author), Adams, H.P.H.M. (author), van Leuven, K. (author), Voskuilen, M. (author), Tesser, G.I. (author), Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)
Fibrin accelerates the activation of plasminogen catalyzed by tissue-type plasminogen activator much stronger than fibrinogen. Detailed studies showed that (part of) this rate-enhancing effect of fibrin is brought about by two sites in the fibrin molecule: one in Aα-(148-160) and one in the γ-chain stretch 311-379 (also known as FCB-5). During...
article 1991
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Rijken, D.C. (author), Groeneveld, E. (author), Gaubius Instituut TNO (author)
Recent studies suggest that plasminogen activators not only hydrolyse a specific arginine-valine bond in plasminogen, but may also cleave other proteins such as fibronectin. We studied the substrate specificity, particularly the preference for arginyl over lysyl peptide bonds, of tissue-type plasminogen activator (t-PA) as well as of two-chain...
article 1991
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Punt, P.J. (author), Zegers, N.D. (author), Busscher, M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)
The expression in Aspergillus is described of genes, coding for intracellular and extracellular proteins controlled by the promoter region of the constitutively and efficiently expressed glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) of Aspergillus nidulans. Both the homologous gpdA and the heterologous Escherichia coli β-galactosidase ...
article 1991
document
Gaubius instituut TNO (author), Schielen, W.J.G. (author), Adams, H.P.H.M. (author), Voskuilen, M. (author), Tesser, G.J. (author), Nieuwenhuizen, W. (author)
The sequence fibrinogen-Aα-(148-160) can mimic part of the fibrin-induced rate enhancement of the activation of plasminogen by tissue-type plasminogen activator. Previously we have reported that the lysine residue at position Aα-157 is crucial. During our further investigations on Aα-157 we found that lysine at position Aα-157 may be replaced by...
article 1991
document
Laman, J.D. (author), Schellekens, M.M. (author), Abacioglu, Y.H. (author), Lewis, G.K. (author), Tersmette, M. (author), Fouchier, R.A.M. (author), Langedijk, J.P.M. (author), Claassen, E. (author), Boersma, W.J.A. (author), Medisch Biologisch Laboratorium TNO (author)
The third variable (V3) domain of the human immunodeficiency virus type 1 (HIV-1) external membrane glycoprotein gp120 is of crucial importance in eliciting neutralizing antibodies in infected persons. Polyclonal (PAb) and monoclonal (MAb) antibodies directed against selected epitopes in the V3 domain are valuable tools for analysis of the...
article 1992
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van Oers, M.M. (author), Malarme, D. (author), Jore, J.P.M. (author), Vlak, J.M. (author), Medisch Biologisch Laboratorium TNO (author)
Two major late proteins, polyhedrin and p10, are synthesized in large quantities in baculovirus infected insect cells. This and the fact that both proteins are dispensable for virus replication, form the basis for the use of these viruses as vector for foreign gene expression. To address the question whether the Autographa californica nuclear...
article 1992
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Punt, P.J. (author), Kramer, C. (author), Kuyvenhoven, A. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author), Medisch Biologisch Laboratorium TNO (author)
Introduction of a previously identified promoter element of the Aspergillus nidulans gpdA gene (encoding glyceraldehyde-3-phosphate dehydrogenase), the so-called gpd box, into the upstream region of the highly regulated A. nidulans amdS gene (encoding acetamidase), significantly increased (up to 30-fold) the expression of the lacZ reporter gene...
article 1992
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Leer, R.J. (author), van Luijk, N. (author), Posno, M. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)
The DNA sequences of a 2.4 kb plasmid (p353-2) from Lactobacillus pentosus MD353 and a 1.9 kb plasmid (p8014-2) from Lactobacillus plantarum ATCC 8014 show 81.5% overall similarity. Both plasmids carry elements (replication protein gene, plus-origin and minus-origin of replication), which are typical of plasmids that replicate via a rolling...
article 1992
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Gerritse, K. (author), de Knijff, P. (author), Ierssel, G.V. (author), Havekes, L.M. (author), Frants, R.R. (author), Schellekens, M.M. (author), Zegers, N.D. (author), Claassen, E. (author), Boersma, W.J.A. (author), Med. Biological Laboratory-TNO, Immunol./Med. Microbiol. Dept. Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
A specific anti-apoE2(Arg158→Cys) monoclonal antibody was raised by means of immunization of mice with a variant specific synthetic peptide. The peptide sequences used were homologous to apolipoprotein E of human and mouse. Consequently, the mouse immune system was tolerant to most of the selected sequences. Immunization with only one of...
article 1992
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Hensgens, L.A.M. (author), Fornerod, M.W.J. (author), Rueb, S. (author), Winkler, A.A. (author), van der Veen, S. (author), Schilperoort, R.A. (author), Centrum voor Plantenfysiologisch Onderzoek (author)
Transcriptional and translational fusions between the reading frame of the β-D-glucuronidase gene (gusA) and the 2′ as well as the 1′ promoter of mannopine synthase (mas), a TR locus of Agrobacterium tumefaciens, were made. The expression of these constructs was studied in the transgenic F1 offspring of independent tobacco transformants at the...
article 1992
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de Pater, Sylvia B. (author), van der Mark, F. (author), Rueb, S. (author), Katagiri, F. (author), Chua, N.H. (author), Schilperoort, R.A. (author), Hensgens, L.A.M. (author), Centrum voor Plantenfysiologisch Onderzoek (author)
A single copy gene has been isolated, termed GOS2, from rice. Sequence comparison revealed highly similar genes in mammals and yeast, indicating that GOS2 encodes an evolutionary conserved protein. GOS2 mRNA was detected in all tissues examined. When the upstream region was translationally fused to the reporter gene gusA it was found to drive...
article 1992
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