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document
Gaubius instituut TNO (author), van Ruijven-Vermeer, I.A.M. (author), Nieuwenhuizen, W. (author), Nooijen, W.J. (author)
The authors studied the binding of Ca to rat fibrinogen and plasmic fibrin(ogen) degradation products by means of equilibrium dialysis with special reference to the protective effect of Ca2+ in the plasmic degradation of fibrinogen. Direct binding studies demonstrate that rat fibrinogen and the plasmic degradation products D(cate) and D-dimer...
article 1978
document
Nieuwenhuizen, W. (author), Vermond, A. (author), Haverkate, F. (author), Gaubius instituut TNO (author)
Experiments have been carried out with fibrinogen and with purified degradation products of fibrinogen and fibrin which demonstrate that the structure of D fragments obtained after prolonged plasmin digestion is influenced by several factors in the media. The previously described protective effect of calcium ions on the ??-chain carboxy...
article 1981
document
Nieuwenhuizen, W. (author), Gravesen, M. (author), Gaubius instituut TNO (author)
Early plasmin degradation products (X fragments) of human fibrinogen were prepared in the presence of calcium-ions or EGTA, and purified on Sepharose 6B-CL. X fragments were characterized with respect to amino-terminal amino acids, polypeptide-chain composition, anticlotting properties and calcium-binding. Amino-terminal amino acids were alanine...
article 1981
document
Nieuwenhuizen, W. (author), Voskuilen, M. (author), Hermans, J. (author), Gaubius instituut TNO (author)
The present study was undertaken as a step to delineate further the localization of the calcium-binding sites in fibrinogen and to assess the anticlotting properties of fibrinogen degradation products. To this purpose, fragments Y were prepared by plasmin digestion of human fibrinogen in the presence of added Ca2+, and purified. We found that,...
article 1982
document
Verheijen, J.H. (author), Nieuwenhuizen, W. (author), Wijngaards, G. (author), Gaubius instituut TNO (author)
Tissue activator-mediated plasminogen activation is potentiated both by fibrin and by some soluble fibrin(ogen) fragments. The potentiating effect of the different fragments decreases in the order fibrin monomer > D-dimer > Y > D EGTA > Dcate > X. Fibrinogen and the fragments Ecate, E EGTA and E fibrin have almost no effect. The existence of a...
article 1982
document
Koppert, P.W. (author), Huijsmans, C.M.G. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)
Spleen cells of BALB/c mice, immunized with fragments Y of normal human fibrinogen, were fused with P3 x 63 Ag 8653 myeloma cells. A clone was found which produces monoclonal antibodies (Mab-Y18) of the IgM?? type. Mab-Y18 is immunoreactive with normal human fibrinogen, and its fragments X, Y, N-terminal disulphide knot, A??-chain, and A??...
article 1985
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Koppert, P.W. (author), Koopman, J. (author), Haverkate, F. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)
Balb/c mice were immunized with a mixture of fibrin degradation products (XDPs) prepared by complete lysis of a human bloot clot by tissue-type plasminogen activator and purified by immunoaffinity chromatography. Spleen cells of the mice were fused with P3 x 63 Ag 8653 myeloma cells. A clone (FDP 14) was selected that produces monoclonal...
article 1986
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van Hinsbergh, V.W.M. (author), Bauer, K.A. (author), Kooistra, T. (author), Kluft, C. (author), Dooijewaard, G. (author), Sherman, M.L. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)
Several investigators have reported that tumor necrosis factor (TNF) can alter the production of plasminogen activator type-1 (PAI-1) and plasminogen activators (PAs) by endothelial cells in vitro. We have examined the in vivo effects of recombinant human TNF administration on fibrinolysis as assessed by parameters in plasma during a 24-hour...
article 1990
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Nieuwenhuizen, W. (author), Gaubius Laboratory Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Increased plasma levels of soluble fibrin are considered as molecular markers of an impending thrombotic event. Several methods to assess soluble fibrin have been in existence for many years. Most of those methods are nonspecific, semiquantitative or too laborious to be used in clinical practice. More recent methods are quantitative and...
article 1993
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van Poppel, G. (author), Hospers, J. (author), Nieuwenhuizen, W. (author), Laterveer, R. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A high intake of β-carotene has been associated with a decreased risk for cardiovascular disease. To evaluate whether β-carotene may exert a protective effect through an impact on haemostasis a randomized, placebo-controlled trial was conducted in male smokers (n = 149) using 20 mg/day β-carotene for 14 weeks. For comparisons, haemostatic...
article 1995
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Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)
The term 'D-dimer assay' suggests, that these assays report the concentrations of the end-stage degradation product of crosslinked fibrin. This hardly occurs in patients. Degradation products of crosslinked fibrin rather occur with a wide range of molecular weights, and comprise various numbers of the D-dimer motif. Moreover, the numerical...
article 1997
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Bos, R. (author), van Leuven, C.J.M. (author), Stolk, J. (author), Hiemstra, P.S. (author), Ronday, H.K. (author), Nieuwenhuizen, W. (author), TNO Preventie en Gezondheid (author)
Upon stimulation, polymorphonuclear leucocytes (PMNs) release potent serine proteases, i.e. elastase, cathepsin C and proteinase 3, which contribute to the degradation of tissue and plasma components. Here, we describe the development of a plasma test to assess PMN-mediated fibrinogenolysis as a biochemical marker for actual PMN-derived...
article 1997
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El-Sayed, M.S. (author), Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)
The present study examined the influence of ingesting a moderate dose of alcohol on plasminogen activator activity (t-PA), plasma fibrinogen (Fb), total degradation products (TDP) and the degradation products of fibrin (FbDP) and fibrinogen (FgDP) at rest and in response to exercise. Eleven male subjects performed two separate experimental...
article 2000
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Lugovskoy, E.V. (author), Kolesnikova, I.N. (author), Gritsenko, P.G. (author), Zolotareva, E.N. (author), Gaffney, P. (author), Nieuwenhuizen, W. (author), Komisarenko, S.V. (author), TNO Preventie en Gezondheid (author)
Monoclonal antibody (mAb) III-3b binds D-dimer with Kd = 1.4 × 10-10 M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bβ134-190, presumably in Bβ155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III...
article 2002
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Rijken, D.C. (author), Hoegee-de Nobel, E. (author), Jie, A.F.H. (author), Atsma, D.E. (author), Schalij, M.J. (author), Nieuwenhuizen, W. (author), TNO Kwaliteit van Leven (author)
Background: The development of global tests for the fibrinolytic capacity in blood is hampered by the low base-line fibrinolytic activity in blood, by the involvement of both plasmatic components and blood cells in the fibrinolytic system and by the loss of fibrinolytic activity as a result of the action of plasminogen activator inhibitor-1 (PAI...
article 2008
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