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Gaubius Instituut TNO (author), Emeis, J.J. (author), Eijnden van den - Schrauwen, Y. (author), van den Hoogen, C.M. (author), de Priester, W. (author), Westmuckett, A. (author), Lupu, F. (author)
In previous studies we have shown that, after stimulation by a receptor ligand such as thrombin, tissue-type plasminogen activator (tPA) and von Willebrand factor (vW(f)) will be acutely released from human umbilical vein endothelial cells (HUVEC). However, the mechanisms involved in the secretion of these two proteins differ in some respects,...
article 1997
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Gruys, E. (author), Tooten, P.C.J. (author), Kuijpers, M.H.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Amyloid deposits represent frequent histological findings in SPF strains of mice mainly used for toxicological studies. Usually, these are deposits of reactive amyloid (AA-amyloid) derived from the acute phase protein serum amyloid A (SAA). The SAA is an apoprotein of high density lipoprotein (apoSAA). Senescence-accelerated amyloid (ASsam)...
article 1996
document
Visser, C.J.T. (author), Rijksen, G. (author), Woutersen, R.A. (author), de Weger, R.A. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
This study investigates the possibility that the c-Src protein tyrosine kinase is involved in experimental exocrine pancreatic carcinogenesis. Expression and activity of the protooncogene pp60c-src (c-Src) are investigated in acinar pancreatic (pre-) neoplastic lesions induced in rats by azaserine and compared with normal rat pancreas. Low or...
article 1996
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Li, K.W. (author), van Minnen, J. (author), van Veelen, P.A. (author), van der Greef, J. (author), Geraerts, W.P.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A neuropeptide that strongly inhibits the spontaneous contractions of the oesophagus in Lymnaea has been characterized as GAPRFVamide. Direct mass spectrometry of nervous tissues and immunocytochemical studies show that the peptide is synthesized by neurones in the buccal ganglia and transported to the oesophagus via the dorso-buccal nerve. In...
article 1996
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Ronday, H.K. (author), Smits, H.H. (author), van Muijen, G.N.P. (author), Pruszczynski, M.S.M. (author), Dolhain, R.J.E.M. (author), van Langelaan, E.J. (author), Breedveld, F.C. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
Proteolytic joint destruction in inflammatory and non-inflammatory arthropathy is believed to be mediated, at least in part, by the plasminogen activation (PA) system. To further investigate possible involvement of the PA system, we quantified immunoreactive urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (t-PA),...
article 1996
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Visser, C.J.T. (author), Bruggink, A.H. (author), Korc, M. (author), Kobrin, M.S. (author), de Weger, R.A. (author), Seifert-Bock, I. (author), van Blokland, W.T.M. (author), van Garderen Hoetmer, A. (author), Woutersen, R.A. (author), TNO Voeding (author)
Using immunohistochemistry, Northern blotting and a semi-quantitative PCR technique, epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and epidermal growth factor receptor (EGFR) expression were studied in the pancreas of N-nitrosobis(2-oxopropyl)-amine (BOP)-treated hamsters. After initiation pancreatic carcinogenesis was...
article 1996
document
TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Visser, C.J.T. (author), de Weger, R.A. (author), van Blokland, W.T.M. (author), Seifert-Bock, I. (author), Kobrin, M.S. (author), Korc, M. (author), Woutersen, R.A. (author)
In the present study, the expression of the epidermal growth factor receptor (EGFR) was investigated in putative preneoplastic and neoplastic acinar cell lesions induced in the rat pancreas by azaserine, using Northern blotting, in situ hybridisation (ISH) and immunohistochemistry. EGFR protein levels were decreased in putative preneoplastic...
article 1996
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Gaubius Instituut TNO (author), Gerritse, K. (author), Laman, J.D. (author), Noelle, R.J. (author), Aruffo, A. (author), Ledbetter, J.A. (author), Boersma, W.J.A. (author), Claassen, E. (author)
We investigated the role of CD40-CD40 ligand (CD40L) interactions in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE). Activated helper T cells expressing CD40L (gp39) surface protein were found in MS patient brain sections, but not in brain tissue sections of normal controls or patients with other neurological diseases....
article 1996
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Wolterbeek, A.P.M. (author), Roggeband, R. (author), Baan, R.A. (author), Feron, V.J. (author), Rutten, A.A.J.J.L. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
Vitamin A and β-carotene protect against respiratory tract cancer by inhibiting the formation of DNA damage and controlling cellular proliferation and differentiation, Recently, it has been shown that the p53 tumor-suppressor gene plays a crucial role in the etiology of respiratory tract cancer. In the present study, we investigated the...
article 1995
document
Hoefakker, S. (author), Balk, H.P. (author), Boersma, W.J.A. (author), van Joost, T. (author), Notten, W.R.F. (author), Claassen, E. (author), TNO Preventie en Gezondheid (author)
Fluorescent contact chemical allergens provoke sensitization after application on both syngeneic and allogeneic skin grafts in mice. We attempted to determine whether the functional activity in a contact sensitization response of human skin graft was affected at the level of antigen uptake and migration. After xenogeneic skin transplantation, we...
article 1995
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Draijer, R. (author), Vaandrager, A.B. (author), Nolte, C. (author), de Jonge, H.R. (author), Walter, U. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Previous studies demonstrated that the thrombin-induced permeability of endothelial cell monolayers is reduced by the elevation of cGMP. In the present study, the presence of cGMP-dependent protein kinase (cGMP-PK) immunoreactivity and activity in various types of human endothelial cells (ECs) and the role of cGMP-PK in the reduction of thrombin...
article 1995
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Hoefakker, S. (author), Boersma, W.J.A. (author), Claassen, E. (author)
article 1995
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Padro, T. (author), Quax, P.H.A. (author), van den Hoogen, C.M. (author), Roholl, P. (author), Verheijen, J.H. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
Plasminogen activator (PA) and PA inhibitor (PAI) antigen, activity, and mRNA were analyzed in the three layers of rat aorta, and the effect of endotoxin on PA and PAI was studied. All PA activity in aorta was identified as tissue-type PA (TPA) activity; no urokinase-type PA was detected. In the tunica adventitia TPA activity, TPA antigen, and...
article 1994
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Lang, M.S. (author), Oostendorp, R.A.J. (author), Simons, P.J. (author), Boersma, W. (author), Knegt, P. (author), van Ewijk, W. (author)
Both retroviral infections as well as human tumors may cause immunosuppression. One of the factors involved in immunosuppression in patients with squamous cell carcinoma of the head and neck (SCC-HN) is a protein related in the retroviral protein p15E. A conserved, 17-amino acid sequence represents the immunosuppressive epitope of retroviral...
article 1994
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Foy, T.M. (author), Laman, J.D. (author), Ledbetter, J.A. (author), Aruffo, A. (author), Claassen, E. (author), Noelle, R.J. (author), Medisch Biologisch Laboratorium TNO (author)
gp39, the ligand for CD40 expressed on activated CD4+ T helper cells, is required for the generation of antibody responses to T-dependent (TD) antigens. Treatment of mice with anti-gp39 in vivo inhibits both primary and secondary antibody formation to TD, but not T-independent antigens. However, the role of this receptor-ligand pair in the...
article 1994
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Vink, A.A. (author), Berg, R.J.W. (author), de Gruijl, F.R. (author), Lohman, P.H.M. (author), Roza, L. (author), Baan, R.A. (author), Centraal Instituut voor Voedingsonderzoek TNO Medisch Biologisch Laboratorium TNO (author)
An immunocytochemical method was developed to study induction and removal of DNA damage in specific cell populations in the epidermis of hairless mice during chronic ultraviolet (UV) exposure. Identification of mouse suprabasal cells was performed with an immunoperoxidase stain. This stain was shown not to affect the fluorescent nuclear stains,...
article 1993
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Instituut voor verouderings- en vaatziekten onderzoek TNO (author), HogenEsch, H. (author), Gijbels, M.J.J. (author), Offerman, E. (author), van Hooft, J. (author), van Bekkum, D.W. (author), Zurcher, C. (author)
Chronic proliferative dermatitis is a new spontaneous mutation in C57BL/Ka mice. Breeding results suggest an autosomal recessive mode of inheritance. Mutant mice develop skin lesions at the age of 5 to 6 weeks. The lesions occur in the ventral and dorsal skin of the body, whereas ears, footpads, and tail are not involved. The lesions are...
article 1993
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Laman, J.D. (author), ter Hart, H. (author), Boorsma, D.M. (author), Claassen, E. (author), van Rooijen, N. (author), Medisch Biologisch Laboratorium TNO (author)
Studies concerning the localization of immune complexes in lymphoid follicles and the involvement of these trapped immune complexes in the regulation of the immune response have thus far been performed with poorly defined complexes in terms of size and composition. For that reason, the minimum requirements for trapping in terms of number of...
article 1992
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Claassen, E. (author), Gerritse, K. (author), Laman, J.D. (author), Boersma, W.J.A. (author), Medisch Biologisch Laboratorium TNO (author)
article 1992
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Laman, J.D. (author), van den Eertwegh, A.J.M. (author), Deen, C. (author), Vermeulen, N. (author), Boersma, W.J.A. (author), Claassen, E. (author), Medisch Biologisch Laboratorium TNO (author)
Synthetic peptide-alkaline phosphatase conjugates can be used to detect the epitope specificity of (i) antibody-forming cells in vivo by immunocytochemistry; (ii) of antibody secreting cells in vitro by spot-ELISA; and (iii) antibodies in solution by capture ELISA. The availability of synthetic peptide-enzyme conjugates using detector enzymes...
article 1991
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