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Maassen, C.B.M. (author), van Holten-Neelen, C. (author), Balk, F. (author), den Bak-Glashouwer, M.-J.H. (author), Leer, R.J. (author), Laman, J.D. (author), Boersma, W.J.A. (author), Claassen, E. (author)
Different Lactobacillus strains are frequently used in consumer food products. In addition, recombinant lactobacilli which contain novel expression vectors can now be used in immunotherapeutic applications such as oral vaccination strategies and in T cell tolerance induction approaches for autoimmune disease. Both for food and clinical...
article 2000
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Graaf, I.A.M. (author), Tajima, O. (author), Groten, J.P. (author), Wolterbeek, A.P.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Gap junctional intercellular communication (GJIC) and cell proliferation were studied in control and 1,1'-bis(p-chlorophenyl)-2,2,2,-trichloroethane (DDT) treated precision-cut liver slices of rat by evaluating connexin 32 (Cx32) expression and 5-bromo-2'-deoxyuridine (BrdU) incorporation. In addition, the effect of different culture media (RPMI...
article 2000
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Department of Pharmacology (author), Langeveld-Wildschut, E.G. (author), Riedl, H. (author), Thepen, T. (author), Bihari, I.C. (author), Bruijnzeel, P.L.B. (author), Bruijnzeel-Koomen, C.A.F.M. (author)
Background: Pharmacologic studies in atopic eczema (AE) are difficult to standardize. Patients with AE differ in the stage of their skin disease (acute, subacute, chronic). Objective: This study was designed to assess macroscopic and microscopic effects of pretreatment with topical glucocortico-steroids (GCSs) and tar on the atopy patch test ...
article 2000
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Kuper, C.F. (author), Schuurman, H.J. (author), Bos-Kuijpers, M. (author), Bloksma, N. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The term autoimmunity refers to physiologically normal immune processes against self-antigens. In rare cases, the regulatory mechanisms become deflective and the uncontrolled production of autoantibodies or activation of autoreactive T-cells can subsequently cause disease. Substances may be capable of evoking autoimmune disease, and it is a...
article 2000
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de Smet, B.J.G.L. (author), de Kleijn, D. (author), Hanemaaijer, R. (author), Verheijen, J.H. (author), Robertus, L. (author), van der Helm, Y.J.M. (author), Borst, C. (author), Post, M.J. (author), Gaubius Instituut TNO (author)
Background - Arterial remodeling after balloon angioplasty has been recognized as a major determinant of restenosis. Perturbation of collagen metabolism might be important. After balloon injury, matrix metalloproteinase (MMP) expression is upregulated. We investigated the effect of Batimastat, a nonspecific MMP inhibitor, on late lumen loss,...
article 2000
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Chen, C.-T. (author), Burton-Wurster, N. (author), Lust, G. (author), Bank, R.A. (author), Tekoppele, J.M. (author)
The first objective of this study was to determine if the cumulative effects of impact or smoothly arising compression would damage the matrix of articular cartilage. Canine cartilage explants were subjected to repeated impacts or to smoothly arising compressions of as much as 20 MPa at 0.3 Hz for as long as 120 minutes. An increase in the water...
article 1999
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Gijbels, M.J.J. (author), van der Cammen, M. (author), van der Laan, L.J.W. (author), Emeis, J.J. (author), Havekes, L.M. (author), Hofker, M.H. (author), Kraal, G. (author), Gaubius instituut TNO (author)
Apolipoprotein E3-Leiden (APOE3-Leiden) transgenic mice develop hyperlipidemia and are highly susceptible to diet-induced atherosclerosis. We have studied the progression and regression of atherosclerosis using immunohistochemistry. Female transgenic mice were fed a moderate fat diet to study atherosclerosis over a longer time period. Fatty...
article 1999
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Voskuil, D.W. (author), Kampman, E. (author), van Kraats, A.A. (author), Balder, H.F. (author), van Muijen, G.N.P. (author), Goldbohm, R.A. (author), van 't Veer, P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Epidemiological studies have suggested that dietary factors may differently affect p53-dependent and p53-independent pathways to colon cancer. Results of such studies may depend on the method used to assess p53 status. This case-control study of 185 colon-cancer cases and 259 controls examines this relation, using both immunohistochemistry and...
article 1999
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Bruder, M.C. (author), Spanhaak, S. (author), Bruijntjes, J.P. (author), Michielsen, C.P.P.C. (author), Vos, J.G. (author), Kuper, C.F. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
In order to study the intestinal mucosal immune cells, with emphasis on single T lymphocytcs, an inventory was made of single and organized lymphocytes in the epithelium and lamina propria of the small intestines of untreated Wistar, Fischer 344, and Lewis rats. The single and organized lymphocytes were examined microscopically. In addition, the...
article 1999
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Maassen, C.B.M. (author), Laman, J.D. (author), Heijne den Bak-Glashouwer, M.J. (author), Tielen, F.J. (author), van Holten-Neelen, J.C.P.A. (author), Hoogteijling, L. (author), Antonissen, C. (author), Leer, R.J. (author), Pouwels, P.H. (author), Boersma, W.J.A. (author), Shaw, D.M. (author)
Lactobacillus strains possess properties that make them attractive candidates as vehicles for oral administration of therapeutics. In this report we describe the construction and analysis of recombinant Lactobacillus casei applicable in oral vaccination against an infectious disease (tetanus) and in oral tolerance induction for intervention in...
article 1999
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TNO Preventie en Gezondheid (author), van Dijk, K.W. (author), van Vlijmen, B.J.M. (author), van 't Hof, H.B. (author), van der Zee, A. (author), Santamarina-Fojo, S. (author), van Berkel, T.J.C. (author), Havekes, L.M. (author), Hofker, M.H. (author)
To investigate the quantitative requirement for apolipoprotein (apo) E in the clearance of lipoproteins via the non-low density lipoprotein (LDL) receptor mediated pathway, human APOE was overexpressed at various levels in the livers of mice deficient for both the endogenous Apoe and Ldlr genes (Apoe-/- · Ldlr-/-) using adenovirus-mediated gene...
article 1999
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Laman, J.D. (author), Maassen, C.B.M. (author), Schellekens, M.M. (author), Visser, L. (author), Kap, M. (author), de Jong, E. (author), van Puijenbroek, M. (author), van Stipdonk, M.J.B. (author), van Meurs, M. (author), Schwärzler, C. (author), Günthert, U. (author), TNO Preventie en Gezondheid (author)
Interactions between mononuclear cells are required for the formation of inflammatory infiltrates in the CNS and the activation of cellular effector functions provoking demyelination in MS. Membrane-expressed costimulatory molecules are crucial to such interactions. We therefore investigated whether two costimulatory molecules, CD40L (CD154,...
article 1998
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Duizer, E. (author), van der Wulp, C. (author), Versantvoort, C.H.M. (author), Groten, J.P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Palmitoyl carnitine chloride (PCC) has been shown to be an effective enhancer of intestinal transport of hydrophilic molecules. The exact mechanism by which the epithelial barrier function is decreased is not clear. In an attempt to elucidate the mechanism of action of PCC, we studied the relationship among absorption enhancement, cell viability...
article 1998
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Konttinen, Y.T. (author), Halinen, S. (author), Hanemaaijer, R. (author), Sorsa, T. (author), Hietanen, J. (author), Ceponis, A. (author), Xu, J.W. (author), Manthorpe, R. (author), Whittington, J. (author), Larsson, Å. (author), Salo, T. (author), Kjeldsen, L. (author), Stenman, U.-H. (author), Eisen, A.Z. (author), Gaubius Instituut TNO (author)
Type IV collagenases/gelatinases (matrix metalloproteinases MMP-2 and MMP-9) in labial salivary glands (LSG) and saliva in Sjogren's syndrome (SS) and healthy controls were studied. Zymograms and Western blots disclosed that SS saliva contained 92/82 kD MMP-9/type IV collagenase duplex. Specific activity measurement disclosed 53.1 ± 9.8 U/mg...
article 1998
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Vyth-Dreese, F.A. (author), Boot, H. (author), Dellemijn, T.A.M. (author), Majoor, D.M. (author), Oomen, L.C.J.M. (author), Laman, J.D. (author), van Meurs, M. (author), de Weger, R.A. (author), de Jong, D. (author), TNO Preventie en Gezondheid (author)
Costimulatory molecules are essential in cognate interactions between T and B lymphocytes. To study the prerequisites of functional interactions between malignant B cells and intermingled T cells in B-cell non-Hodgkin's lymphomas (B-NHL), we examined the expression of CD40, CD80 and CD86 and their ligands CD40 ligand (CD40L, CD154), CD28 and...
article 1998
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Blommaert, F.A. (author), Floot, B.G.J. (author), van Dijk-Knijnenburg, H.C.M. (author), Berends, F. (author), Baan, R.A. (author), Schornagel, J.H. (author), den Engelse, L. (author), Fichtinger-Schepman, A.M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
We have studied the formation and repair of cisplatin-DNA adducts in wild-type mouse leukemia L1210/0 cells and in the sublines L1210/2 and L1210/5, which differ in cisplatin sensitivity. In a colony-formation assay these sublines were 9- and 22-fold more resistant compared to L1210/0, respectively. Cisplatin-induced DNA modification was studied...
article 1998
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Noorman, F. (author), Braat, E.A.M. (author), Barrett-Bergshoeff, M.M. (author), Barbé, E. (author), van Leeuwen, A. (author), Lindeman, J. (author), Rijken, D.C. (author), TNO Preventie en Gezondheid (author)
Recently we developed mouse monocloual antibodies (mAb) against the isolated human 175-kDa mannose receptor. In the present study we tested whether these mAb are suitable for the detection of the mannose receptor on cultured macrophages using flow cytometry and on cells in human tissues using immunohistochemistry. Human monocytes did not react...
article 1997
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Lupu, C. (author), Goodwin, C.A. (author), Westmuckett, A.D. (author), Emeis, J.J. (author), Scully, M.F. (author), Kakkar, V.V. (author), Lupu, F. (author), Gaubius Instituut TNO (author)
Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor·factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC...
article 1997
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Bajramović, J.J. (author), Lassmann, H. (author), van Noort, J.M. (author), TNO Preventie en Gezondheid (author)
The small heat shock protein αB-crystallin was recently identified as a dominant human T-cell antigen in myelin derived from multiple sclerosis (MS) patients. Using immunohistochemical techniques, oligodendrocytes as well as astrocytes in MS lesions were shown to express α B-crystallin. In the present study we examined the expression of α B...
article 1997
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Barrett-Bergshoeff, M.M. (author), Moorman, F. (author), Bos, R. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
To study the role of the mannose receptor in cellular uptake and degradation of tissue-type plasminogen activator (t-PA), a set of five monoclonal antibodies (Moab) was generated against the mannose receptor isolated from human placental tissue. All Moab specifically recognised the 175 kDa mannose receptor in a crude placenta extract, as shown...
article 1997
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