- document
-
Hau, M.-F. (author), Smelt, A.H.M. (author), Bindels, A.J.G.H. (author), Sijbrands, E.J.G. (author), van der Laarse, A. (author), Onkenhout, W. (author), van Duyvenvoorde, W. (author), Princen, H.M.G. (author), Gaubius Instituut TNO (author)In hypertriglyceridemic (HTG) patients the addition of fish oil to the diet causes a marked reduction in the concentration of triglyceride-rich lipoproteins in the serum. To investigate the effects of fish oil on the oxidation resistance of VLDL and LDL in HTG patients, nine male patients received 1 g/d fish oil (containing 55.7% n-3...article 1996
- document
-
Groen, E. (author), de Graaf, B. (author), Bles, W. (author), Bos, J.E. (author), TNO Technische Menskunde (author)To assess a possible otolith contribution to effects observed following prolonged expo-sure to hyper gravity, we used video-oculography to measure ocular torsion during static and dynamic conditions of lateral body tilt (roll) before and after one hour of centrifugation with a Gx-load of 3G. Static tilt (from 0 to 57 deg. to either side) showed...article 1996
- document
-
Hartmann, D. (author), Güzelhan, C. (author), Zuiderwijk, P.B.M. (author), Odink, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Objectives: Orlistat, a potent and selective inhibitor of gastrointestinal lipases, is designed for the treatment of obesity. A double-blind, randomised, placebo-controlled, 2-way crossover study investigated the possible influence of orlistat on the ovulation-suppressing action of combination oral contraceptives (OC). Methods: After an 8-day...article 1996
- document
-
van Iersel, M.L.P.S. (author), Ploemen, J.P.H.T.M. (author), Struik, I. (author), van Amersfoort, C. (author), Keyzer, A.E. (author), Schefferlie, J.G. (author), van Bladeren, P.J. (author), TNO Voeding (author)The glutathione S-transferase (GST) activity towards 1-chloro-2,4-dinitrobenzene in intact human IGR-39 melanoma cells was determined by the quantification by HPLC-analysis of the excreted glutathione (GSH) conjugate (S-(2,4-dinitrophenyl)glutathione; DNPSG). The major GST subunit expressed in these melanoma cells is the π-class GST subunit P1....article 1996
- document
-
Rompelberg, C.J.M. (author), Ploemen, J.H.T.M. (author), Jespersen, S. (author), van der Greef, J. (author), Verhagen, H. (author), van Bladeren, P.J. (author), TNO Voeding (author)The irreversible and reversible inhibition of glutathione S-transferases (GSTs) by eugenol was studied in rat, mouse and man. Using liver cytosol of human, rat and mouse, species differences were found in the rate of irreversible inhibition of GSTs by eugenol in the presence of the enzyme tyrosinase. Tyrosinase was used to oxidize eugenol. No...article 1996
- document
-
Remarque, E.J. (author), Nijhuis, E.W.P. (author), Hinloopen, B. (author), Nagelkerken, L. (author), van der Velde, E.A. (author), Ligthart, G.J. (author), Instituut voor Experimentele Gerontologie TNO (author)To investigate the effects of the altered composition of the helper T cell compartment in ageing on the humoral response to influenza vaccine, we investigated correlations between helper T cell subsets and anti-influenza antibody responses in 23 JUNIEUR healthy young and 41 SENIEUR healthy elderly subjects. Naive helper T cell numbers (CD4...article 1996
- document
-
Noort, D. (author), Verheij, E.R. (author), Hulst, A.G. (author), de Jong, L.P.A. (author), Benschop, H.P. (author), Prins Maurits Laboratorium TNO TNO Voeding (author)In this paper we describe the use of tandem mass spectrometry to identify modified sites in human hemoglobin after in vitro exposure to bis(2- chloroethyl) sulfide (sulfur mustard). Globin isolated from human whole blood which had been exposed to sulfur mustard was degraded with trypsin, and the digests were analyzed by micro LC/MS. Alkylated...article 1996
- document
-
Offermans, M.T.C. (author), Struyk, L. (author), de Geus, B. (author), Breedveld, F.C. (author), van den Elsen, P.J. (author), Rozing, J. (author), TNO Preventie en Gezondheid (author)In order to define the extent of T cell heterogeneity and clonality, unique DNA sequences in the junctional region in rearranged T cell receptor (TcR) genes can be studied. For this purpose we have adapted a non-denaturing nucleic acid gel electrophoresis procedure to detect TcR junctional diversity. Detection of junctional diversity is based...article 1996
- document
-
Instituut CIVO-Toxicologie en Voeding TNO (author), Zaman, G.J.R. (author), Cnubben, N.H.P. (author), van Bladeren, P.J. (author), Evers, R. (author), Borst, P. (author)The multidrug resistance protein MRP has been shown to mediate the transport of glutathione S-conjugates across membranes. In this study we demonstrate that the glutathione S-conjugate of the diuretic drug ethacrynic acid, which is an efficient inhibitor of glutathione S-transferases, is a high-affinity substrate and inhibitor of the glutathione...article 1996
- document
-
TNO Preventie en Gezondheid Gaubius Instituut TNO (author), Biessen, E.A.L. (author), Noorman, F. (author), van Teijlingen, M.E. (author), Kuiper, J. (author), Barrett-Bergshoeff, M.M. (author), Bijsterbosch, M.K. (author), Rijken, D.G. (author), van Berkel, T.J.G. (author)In search of synthetic high affinity ligands for the mannose receptor, we synthesized a series of lysine-based oligomannosides containing two (M2L) to six (M6L5) terminal alpha-D-mannose groups that are connected with the backbone by flexible elongated spacers (16 Å). The synthesized cluster mannosides were all able to displace binding of...article 1996
- document
-
Gaubius Instituut TNO (author), Sakharov, D.V. (author), Lijnen, H.R. (author), Rijken, D.C. (author)Staphylokinase (STA), a protein of bacterial origin, induces highly fibrin-specific thrombolysis both in human plasma in vitro and in pilot clinical trials. Using fluorescence microscopy, we investigated the spatial distribution of fluorescein isothiocyanate (FITC)-labeled STA during lysis of a plasma clot and its binding to purified fibrin...article 1996
- document
-
TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), van Dokkum, W. (author), dela Guéronnière, V. (author), Schaafsma, G. (author), Bouley, C. (author), Luten, J. (author), Latgé, C. (author)True fractional Ca absorption from six foods was measured in twelve normal healthy women, aged 20-29 years. The tested foods were commercially available fresh cheese, fresh cheese prepared by new technology and rich in Ca, similar cheese with added Fe, enteral food, mineral water alone and combined with a spaghetti meal. The aim of the study was...article 1996
- document
-
TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Dulfer, W.J. (author), Groten, J.P. (author), Govers, H.A.J. (author)Polychlorinated biphenyls (PCBs) dissolved in dietary fat are absorbed in the gastrointestinal tract by the enterocytes in combination with the fatty acid proceeding from the lipid hydrolysis in the gut lumen. The effect of fatty acid absorption on the uptake and transport of 14 PCBs in enterocytes was studied using monolayers of the human...article 1996
- document
-
TNO Preventie en Gezondheid Gaubius Instituut TNO (author), Sier, C.F.M. (author), Kubben, F.J.G.M. (author), Ganesh, S. (author), Heerding, M.M. (author), Griffioen, G. (author), Hanemaaijer, R. (author), van Krieken, J.H.J.M. (author), Lamers, C.B.H.W. (author), Verspaget, H.W. (author)Proteinases are involved in tumour invasion and metastasis. Several matrix metalloproteinases (MMPs) have been shown to be increased in various human carcinomas. We assessed the levels of MMP-2 (gelatinase A) and MMP-9 (gelatinase B) in 50 gastric carcinomas and corresponding mucosa using quantitative gelatin zymography. Both MMP levels were...article 1996
- document
-
TNO Preventie en Gezondheid (author), Biasucci, L.M. (author), Liuzzo, G. (author), Caligiuri, G. (author), Quaranta, G. (author), Andreotti, F. (author), Sperti, G. (author), van de Greef, W. (author), Rebuzzi, A.G. (author), Kluft, C. (author), Maseri, A. (author)Background: Although a major role of coronary thrombosis in the pathogenesis of unstable angina has been demonstrated, the results of a series of studies have suggested that activation of the hemostatic system may not be confined to ischemic episodes. The purpose of this study was to investigate the temporal relation between ischemic episodes...article 1996
- document
-
TNO Preventie en Gezondheid Gaubius Instituut TNO (author), Sakharov, D.V. (author), Nagelkerkel, J.F. (author), Rijken, D.C. (author)Binding of components of the fibrinolytic system to fibrin is important for the regulation of fibrinolysis. In this study, decomposition of the fibrin network and binding of plasminogen and plasminogen activators (PAs) to fibrin during lysis of a plasma clot were investigated with confocal microscopy using fluorescein-labeled preparations of...article 1996
- document
-
Gaubius Instituut TNO (author), Gerritse, K. (author), Laman, J.D. (author), Noelle, R.J. (author), Aruffo, A. (author), Ledbetter, J.A. (author), Boersma, W.J.A. (author), Claassen, E. (author)We investigated the role of CD40-CD40 ligand (CD40L) interactions in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE). Activated helper T cells expressing CD40L (gp39) surface protein were found in MS patient brain sections, but not in brain tissue sections of normal controls or patients with other neurological diseases....article 1996
- document
-
Jansen, J.T.M. (author), Geleijns, J. (author), Zweers, D. (author), Schultz, F.W. (author), Zoetelief, J. (author)The variation in computed tomography dose index (CTDI) to effective dose conversion factors between different types of CT scanner is large (i.e. a factor of about 2 due to differences in beam shaping filters). Consequently, scanner specific conversion factors have to be applied. For some types of scanner, however, detailed information on the...article 1996
- document
-
Centrum voor Stralingsbescherming en Dosimetrie TNO (author), Raaijmakers, C.P.J. (author), Watkins, P.R.D. (author), Nottelman, E.L. (author), Verhagen, H.W. (author), Jansen, J.T.M. (author), Zoetelief, J. (author), Mijnheer, B.J. (author)To obtain a high accuracy in the dosimetry of an epithermal neutron beam used for boron neutron capture therapy (BNCT), the neutron sensitivity of dosimeters applied to determine the various dose components in-phantom has been investigated. The thermal neutron sensitivity of Mg(Ar) ionization chambers, TE(TE) ionization chambers, and...article 1996
- document
-
TNO Preventie en Gezondheid (author), Hirasing, R.A. (author), Bolk-Bennink, L. (author), Reus, H. (author)Purpose: We assessed results of intensive dry bed training for groups of parents outside the hospital setting. Materials and Methods: During 5 consecutive meetings 2 appointed coaches were responsible for providing 5 to 10 parents with detailed instructions outlining the dry bed training program for their child. The 91 participating children ...article 1996