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Jong, M.C. (author), Dahlmans, V.E.H. (author), van Gorp, P.J.J. (author), Breuer, M.L. (author), Mol, M.J.T.M. (author), van der Zee, A. (author), Frants, R.R. (author), Hofker, M.H. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)Transgenic mice overexpressing human APOE*3Leiden are highly susceptible to diet-induced hyperlipoproteinemia and atherosclerosis due to a defect in hepatic uptake of remnant lipoproteins. In addition to the human APOE*3Leiden gene, these mice carry the human APOC1 gene (APOE*3Leiden- C1). To investigate the possible effect of simultaneous...article 1996
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Arts, J. (author), Grimbergen, J. (author), Bosma, P.J. (author), Rahmsdorf, H.J. (author), Kooistra, T. (author), Gaubius Laboratory, TNO-PG, Leiden, Netherlands Institut für Genetik, Forschungszentrum Karlsruhe, Germany TNO Preventie en Gezondheid (author)Experiments were designed to clarify the role of c-Jun/c-Fos and of putative phorbol 12-myristate-13-acetate-(PMA)-responsive elements (TREs) in the induction of plasminogen-activator inhibitor 1 (PAI-1) gene transcription in the human hepatoma cell line HepG2 by activators of protein kinase C (PKC). Treatment of HepG2 cells with the phorbol...article 1996
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Gizdulich, P. (author), Imholz, B.P.M. (author), van den Meiracker, A.H. (author), Parati, G. (author), Wesseling, K.H. (author), TNO Biomedical Instrumentation (author)Objective. Arterial pressure waveforms distort between brachial and finger arteries, causing differences mainly in systolic pressure. Distortion, reportedly, can be removed by applying a waveform filter to the finger pressure. Design. We analysed the data from two studies that detected discrepancies in systolic tracking between Finapres and...article 1996
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van Poppel, G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Objective and conclusions: This article gives an overview of observational and experimental epidemiological studies relating β-carotene to risk of cancer and cardiovascular disease. Observational epidemiological studies have consistently shown that a diet rich in β-carotenerich fruits and vegetables or high blood levels of β-carotene are...article 1996
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Verhagen, H. (author), Rauma, A.L. (author), Törrönen, R. (author), de Vogel, N. (author), Bruijntjes-Rozier, G.C.D.M. (author), Dreve, M.A. (author), Bogaards, J.J.P. (author), Mykkänen, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)1. In order to study the potential beneficial effects of a vegan diet, a cross-sectional study was performed and several biomarkers of chemoprevention were measured in a population of female 'living food' eaters ('vegans'; n = 20) vs matched omnivorous controls (n = 20). 2. White blood cells obtained from fresh blood samples were subjected to...article 1996
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van de Vijver, L.P.L. (author), van Poppel, G. (author), van Houwelingen, A. (author), Kruyssen, D.A.C.M. (author), Hornstra, G. (author)A high intake of trans fatty acids (TFAs) has been shown to have an undesirable effect on serum lipid profiles and lipoprotein(a) (Lp(a)) levels and may thereby increase the risk for coronary heart disease (CHD). We performed a study in CHD patients, and measured the TFA concentration of the plasma phospholipid fraction. Comparison was made...article 1996
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Wormhoudt, L.W. (author), Ploemen, J.H.T.M. (author), de Waziers, I. (author), Commandeur, J.N.M. (author), Beaune, P.H. (author), van Bladeren, P.J. (author), Vermeulen, N.P.E. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)1,2-Dibromoethane (1,2-DBE) is mainly used as an additive in leaded gasoline and as a soil fumigant and it is a suspected carcinogen in humans. In this study, the oxidative bioactivation of 1,2-DBE to 2-bromoacetaldehyde (2-BA) was studied using heterologously expressed human cytochrome P450 (P450) isoenzymes and human liver microsomes. Out of...article 1996
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Schweitz, R. (author), Mopurgo, R. (author), Sietaram, M.A. (author), Hirasing, R.A. (author), TNO Preventie en Gezondheid (author)Objective: To determine the extent and severity of malnutrition in children in the last 10 years in 's Lands Hospitaal. Setting: Paramaribo, Surinam. Design: Retrospective descriptive study. Method: Data were collected from the medical files of children admitted because of malnutrition, by completing a standard questionnaire. Results: The number...article 1996
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van Vliet, A.K. (author), Nègre-Arrariou, P. (author), van Thiel, G.C.F. (author), Bolhuis, P.A. (author), Cohen, L.H. (author), TNO Preventie en Gezondheid (author)Lovastatin, simvastatin, and pravastatin are fairly strong inhibitors of sterol synthesis in human myoblasts in culture. Lovastatin and simvastatin have IC50 values of 19 ± 6 nM and 4.0 ± 2.3 nM, respectively. Pravastatin is a weaker inhibitor of sterol synthesis (IC50 value of 110 ± 38 nM). Through inhibition of mevalonate production, these...article 1996
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de Graaf, B. (author), Bos, J.E. (author), Groen, E.L. (author), TNO Technische Menskunde (author)When someone is tilted laterally the sheer force on the maculae of the utriculus and the sacculus is described by the sine and the cosine of the angle of tilt, respectively. So both the sacculus and the utriculus are stimulated, but in the litera-ture ocular torsion is normally attributed to utricular function alone (and thus seen as a response...article 1996
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Laman, J.D. (author), Claassen, E. (author), Noelle, R.J. (author), TNO Preventie en Gezondheid (author)Initially, a role for the interaction between CD40, expressed on B cells, and gp39 (CD40L), expressed on activated T cells, has been defined in humoral immunity. CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching. CD40-CD40L interaction is...article 1996
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Rijken, D.C. (author), Jie, A.F.H. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)Reteplase is a protein consisting of the kringle-2 and protease domains of tissue-type plasminogen activator (t-PA). Because intravenous heparin will be used as an adjunct to thrombolytic therapy with reteplase, we investigated the interactions in vitro between heparin and reteplase as well as between heparin and recombinant t-PA (alteplase) as...article 1996
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van den Akker, Th.W. (author), Radl, J. (author), Franken-Postma, E. (author), Hagemeijer, A. (author), Gaubius Instituut TNO (author)Multiple myeloma (MM) and Waldenström's macroglobulinemia-like lymphoma (MW) appear spontaneously in C57BL/KaLwRij mice at a frequency of 0.5% and 0.2%, respectively. They can readily be propagated by intravenous transfer of mainly bone marrow or spleen cells into syngeneic recipients. Previous studies demonstrated that these mouse malignant...article 1996
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Kulig, B.M. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)Significant progress has been made in recent years in terms of both the conceptualization of neurotoxicity assessment strategies as well as in the development of behavioral techniques for evaluating neurotoxic exposures. A tiered approach, for example, has been advocated as an assessment strategy in which testing would proceed in a stepwise...article 1996
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Groen, E.L. (author), Bos, J.E. (author), Nacken, P.F.M. (author), de Graaf, B. (author), TNO Technische Menskunde (author)A new, automatic method for determination of human ocular torsion (OT) was devel-oped based on the tracking of iris patterns in digitized video images. Instead of quanti-fying OT by means of cross-correlation of circular iris samples, a procedure commonly applied, this new method automatically selects and recovers a set of 36 significant...article 1996
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Haddeland, U. (author), Sletten, K. (author), Bennick, A. (author), Nieuwenhuizen, W. (author), Brosstad, F. (author), Gaubius Instituut TNO (author)The present paper shows that conformationally changed fibrinogen can expose the sites Aα-(148-160) and γ-(312-324) involved in stimulation of the tissue-type plasminogen activator (t-PA)-catalysed plasminogen activation. The exposure of the stimulating sites was determined by ELISA using mABs directed to these sites, and was shown to coincide...article 1996
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Mutafova, M. (author), van de Water, H.P.A. (author), Perenboom, R.J.M. (author), Boshuizen, H.C. (author), Maleshkov, C. (author), TNO Preventie en Gezondheid (author)This article presents health expectancy calculations from Bulgaria for 1976-1992. The calculations are based on mortality statistics and data from a national information system from the Expert Medical Commissions on Working Capacity about loss of working capacity. Following internationally accepted terminology, the most appropriate term for the...article 1996
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Philip, P.A. (author), Souliotis, V.L. (author), Harris, A.L. (author), Salisbury, A. (author), Tates, A.D. (author), Mitchell, K. (author), van Delft, J.H.M. (author), Ganesan, T.S. (author), Kyrtopoulos, S.A. (author), TNO Voeding (author)Dacarbazine (DTIC) is a DNA-methylating drug used in the treatment of malignant melanoma. Among the DNA dducts induced by DTIC are N7-methylguanine (N7-meG) and O6-methylguamne (O6-meG). The latter adduct, in particular, may be important in the mutagenic as well as the cytotoxic activity of DTIC. Repair of O6-meG is carried out by the enzyme O6...article 1996
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Gouka, R.J. (author), Punt, P.J. (author), Hessing, J.G.M. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)A study was carried out to obtain more insight into the parameters that determine the secretion of heterologous proteins from filamentous fungi. A strategy was chosen in which the mRNA levels and protein levels of a number of heterologous genes of different origins were compared. All genes were under control of the Aspergillus awamori 1,4-β...article 1996
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Massé, P.G. (author), van den Berg, H. (author), Duguay, C. (author), Beaulieu, G. (author), Simard, J.M. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)The objectives of the study were to follow-up six artificial menstrual cycles induced by Triphasil® in order to determine: 1) the time of apparition of B6 metabolic side-effects, in the eventuality they occur, and 2) the existence or non-existence of a normalization process and if so, when it is initiated. The choice of this triphasic OC...article 1996