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van Helden, H.P.M. (author), van der Wiel, H.J. (author), Zijlstra, J.J. (author), Melchers, B.P.C. (author), Busker, R.W. (author)
The oximes HI-6, HLo-7, HGG-12, HGG-42 and obidoxime were used in a previously developed rat model to evaluate the therapeutic effects of oximes other than acetylcholinesterase (AChE) reactivation (so-called 'nonreactivating effects'). To test this anaesthetized, atropinized and artificially ventilated rats (n = 8 or 16) were poisoned with a...
article 1994
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Twisk, J. (author), Hoekman, M.F.M. (author), Lehmann, E.M. (author), Meijer, P. (author), Mager, W.H. (author), Princen, H.M.G. (author), TNO Preventie en Gezondheid (author)
Evidence from in vivo studies indicates that the bile acid pool and bile acid excretion are increased in humans with diabetes mellitus and in experimental diabetic animals, and that both parameters return to normal levels after administration of insulin. To investigate the biochemical background of these changes, the effects of insulin on bile...
article 1995
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Dobber, R. (author), Tielemans, M. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)
In the present study we investigated whether age-related changes in the composition and functional properties of murine CD4+ T cells are reflected in vivo by a changed humoral response to influenza vaccine in aged mice. After the primary immunization, the titers of influenza-specific IgM, IgG1, IgG2a, and IgG2b, but not of IgG3 and IgE, were...
article 1995
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Coenen, T.M.M. (author), Schoenmakers, A.C.M. (author), Verhagen, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Barlican, a β-glucanase enzyme obtained from Trichoderma reesei, was produced by a fermentation process and subjected to a series of toxicological tests to document its safety for use as a feed additive. The enzyme product was examined for general oral toxicity, inhalation toxicity, irritation to eye and skin, skin sensitization and mutagenic...
article 1995
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Ploemen, J.-P.H.T.M. (author), van Iersel, M.L.P.S. (author), Wormhoudt, L.W. (author), Commandeur, J.N.M. (author), Vermeulen, N.P.E. (author), van Bladeren, P.J. (author)
The drug disulfiram (DSF, Antabuse®) has been used in the therapy of alcohol abuse. It is a potent inhibitor of aldehyde dehydrogenase. Its reduced form, diethyldithiocarbamate (DDTC), and further metabolites show similar activities. DSF and DDTC have also been widely used to inhibit mixed-function oxidases. In this study, the reversible...
article 1996
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Clary, J.J. (author), Feron, V.J. (author), van Velthuijsen, J.A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Lactate eaters have an oral LD50 greater than 2000 mg/kg and the inhalation LC50 is generally above 5000 mg/m3 and they may be potential eye and skin irritants, but not skin sensitizers. No evidence of teratogenicity or maternal toxicity was observed in an inhalation (2-ethylhexyl-L-lactate) or dermal study (ethyl-L-lactate). Subacute inhalation...
article 1998
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Chaillou, S. (author), Bor, Y.-C. (author), Batt, C.A. (author), Postma, P.W. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H...
article 1998
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Peschel, A. (author), Jack, R.W. (author), Otto, M. (author), Collins, L.V. (author), Staubitz, P. (author), Nicholson, G. (author), Kalbacher, H. (author), Nieuwenhuizen, W.F. (author), Jung, G. (author), Tarkowski, A. (author), van Kessel, K.P.M. (author), van Strijp, J.A.G. (author)
Defensins, antimicrobial peptides of the innate immune system, protect human mucosal epithelia and skin against microbial infections and are produced in large amounts by neutrophils. The bacterial pathogen Staphylococcus aureus is insensitive to defensins by virtue of an unknown resistance mechanism. We describe a novel staphylococcal gene, mprF...
article 2001
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Versantvoort, C.H.M. (author), Ondrewater, R.C.A. (author), Duizer, E. (author), van de Sandt, J.J.M. (author), Gilde, A.J. (author), Groten, J.P. (author), TNO Voeding (author)
Purpose: previous studies have shown that the rat small intestinal cell line IEC-18 provides a size-selective barrier for paracellularly transported hydrophilic macromolecules. In order to determine the utility of IEC-18 cells as an in vitro model to screen the passive paracellular and transcellular components of the intestinal transport of...
article 2002
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Artola-Garicano, E. (author), Sinnige, T.L. (author), van Holsteijn, I. (author), Vaes, W.H.J. (author), Hermens, J.L.M. (author)
In the current study, the bioconcentration behavior and acute toxicity of two polycyclic musks, Tonalide® 7-acetyl-1,1,3,4,4,6,-hexamethyl-1,2,3,4,-tetrahydronaphthalene (AHTN) and Galaxolide® 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexa-methyl-cyclopenta[γ]-2- benzopyran (HHCB), were studied in two benthic organisms. Polycyclic musks are frequently...
article 2003
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Nieuwenhuizen, W.F. (author), Dekker, H.L. (author), de Koning, L.J. (author), Gröneveld, T. (author), de Koster, C.G. (author), de Jong, G.A.H. (author)
The molecular structures determine the physical properties of milk proteins and are important for the texture of many dairy-based foods. Bovine α-lactalbumin (α-LA) is a globular 123 amino acid Ca2+ binding milk protein. Modification with microbial Ca2+ independent transglutaminase (MTGase) was used to modify lysines and glutamines in holo and...
article 2003
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Joosten, V. (author), Lokman, C. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Voeding (author)
In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of...
article 2003
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Koppelman, S.J. (author), Knol, E.F. (author), Vlooswijk, R.A.A. (author), Wensing, M. (author), Knulst, A.C. (author), Hefle, S.L. (author), Gruppen, H. (author), Piersma, S. (author)
Background: Peanut allergen Ara h 3 has been the subject of investigation for the last few years. The reported data strongly depend on recombinant Ara h 3, since a purification protocol for Ara h 3 from peanuts was not available. Methods: Peanut allergen Ara h 3 (glycinin), was purified and its posttranslational processing was investigated. Its...
article 2003
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Arand, M. (author), Hallberg, B.M. (author), Zou, J. (author), Bergfors, T. (author), Oesch, F. (author), van der Werf, M.J. (author), de Bont, J.A.M. (author), Jones, T.A. (author), Mowbray, S.L. (author)
Epoxide hydrolases are essential for the processing of epoxide-containing compounds in detoxification or metabolism. The classic epoxide hydrolases have an α/β hydrolase fold and act via a two-step reaction mechanism including an enzyme-substrate intermediate. We report here the structure of the limonene-1,2-epoxide hydrolase from Rhodococcus...
article 2003
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van der Mei, H.C. (author), van de Belt-Gritter, B. (author), Pouwels, P.H. (author), Martinez, B. (author), Busscher, H.J. (author), TNO Voeding (author)
Cell surface hydrophobicity is one of the most important factors controlling adhesion of microorganisms to surfaces. In this paper, cell surface properties of lactobacilli and recombinant lactobacilli with and without a surface layer protein (SLP) associated with cell surface hydrophobicity were determined, including water contact angles, zeta...
article 2003
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Heijne, W.H.M. (author), Stierum, R.H. (author), Slijper, M. (author), van Bladeren, P.J. (author), van Ommen, B. (author)
Toxicogenomics is a novel approach integrating the expression analysis of thousands of genes (transcriptomics) or proteins (proteomics) with classical methods in toxicology. Effects at the molecular level are related to pathophysiological changes of the organisms, enabling detailed comparison of mechanisms and early detection and prediction of...
article 2003
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Punt, P.J. (author), Drint-Kuijvenhoven, A. (author), Lokman, B.C. (author), Spencer, J.A. (author), Jeenes, D. (author), Archer, D.A. (author), van den Hondel, C.A.M.J.J. (author)
We have characterized growth and protein processing characteristics of Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains...
article 2003
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Nieuwenhuizen, W.F. (author), Pieters, R.H.H. (author), Knippels, L.M.J. (author), Jansen, M.C.J.F. (author), Koppelman, S.J. (author)
Coeliac disease is a T-cell-mediated autoimmune disease of the small intestine that is induced by ingestion of gluten proteins from wheat, barley, or rye. We postulate that Candida albicans is a trigger in the onset of coeliac disease. The virulence factor of C albicans - hyphal wall protein 1 (HWP1) - contains aminoacid sequences that are...
article 2003
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van Beek, E.R. (author), Cohen, L.H. (author), Leroy, I.M. (author), Ebetino, F.H. (author), Löwik, C.W.G.M. (author), Papapoulos, S.E. (author), Gaubius Instituut TNO (author)
Bisphosphonates (BPS) inhibit bone resorption and are divided into two classes according to their chemical structure and mechanism of action: nonnitrogen containing BPS such as etidronate and clodronate that are of low potency and inhibit osteoclast function via metabolism into toxic ATP-metabolites and nitrogen-containing BPS (NBPS), such as...
article 2003
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Wortelboer, H.M. (author), Usta, M. (author), van der Velde, A.E. (author), Boersma, M.G. (author), Spenkelink, B. (author), van Zanden, J.J. (author), Rietjens, I.M.C.M. (author), van Bladeren, P.J. (author), Cnubben, N.H.P. (author)
The multidrug resistance proteins MRP1 and MRP2 are efflux transporters with broad substrate specificity, including glutathione, glucuronide, and sulfate conjugates. In the present study, the interaction of the dietary polyphenol curcumin with MRP1 and MRP2 and the interplay between curcumin-dependent MRP inhibition and its glutathione-dependent...
article 2003
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