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Hermens, A.F. (author), Bentvelzen, P.A.J. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)
Rat R2k rhabdomyosarcoma cells were transfected with the human H-ras oncogene, which resulted in increased resistance to cell kill in vitro by a single dose of 137Cs γ-rays. A subline carrying one oncogene showed an increase in the quasi-threshold dose (D(q)) from 0.88 to 1.48 Gy. Another subline containing six oncogenes not only had an...
article 1992
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Instituut voor verouderings- en vaatziekten onderzoek TNO (author), van de Kar, N.C.A.J. (author), Monnens, L.A.H. (author), Karmali, M.A. (author), van Hinsbergh, V.W.M. (author)
The epidemic form of the hemolytic uremic syndrome (HUS), beginning with an acute gastroenteritis, has been associated with a verocytotoxin-producing Escherichia coli infection. The endothelial cell is believed to play an important role in the pathogenesis of HUS. Endothelial cell damage by verocytotoxin-1 (VT-1) in vitro is potentiated by the...
article 1992
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van Vliet, A.K. (author), van Thiel, G.C.F. (author), Huisman, R.H. (author), Moshage, H. (author), Yap, S.H. (author), Cohen, L.H. (author), TNO Preventie en Gezondheid (author)
The three vastatins examined, lovastatin, simvastatin and pravastatin, are equally strong inhibitors of the sterol synthesis in human hepatocytes in culture with IC50-values of 4.1, 8.0 and 2.0 nM, respectively. However, in the human extrahepatic cells: umbilical vascular endothelial cells, retinal pigment epithelial cells, cornea fibroblasts...
article 1995
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van de Kar, N.C.A.J. (author), Kooistra, T. (author), Vermeer, M. (author), Lesslauer, W. (author), Monnens, L.A.H. (author), van Hinsbergh, V.W.M. (author), TNO Preventie en Gezondheid (author)
Infections with verocytotoxin (VT) producing Escherichia coil have been strongly implicated in the epidemic form of hemolytic uremic syndrome (HUS). Endothelial damage plays a central role in the pathogenesis of HUS. In vitro studies have shown that VT can damage endothelial cells after interaction with its cellular receptor...
article 1995
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Draijer, R. (author), Atsma, D.E. (author), van der Laarse, A. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Previous studies have demonstrated that cGMP and cAMP reduce the endothelial permeability for fluids and macromolecules when the endothelial permeability is increased by thrombin. In this study, we have investigated the mechanism by which cGMP improves the endothelial barrier function and examined whether nitric oxide (NO) can serve as an...
article 1995
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Arts, J. (author), Lansink, T. (author), Grimbergen, J. (author), Toet, K.H. (author), Kooistra, T. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
We have previously shown that the pleiotropic agent sodium butyrate strongly stimulates tissue-type plasminogen activator (t-PA) expression in human umbilical vein endothelial cells (HUVEC). Here we provide the following evidence that the butyrate-induced t-PA expression in HUVEC involves histone H4 acetylation. (1) t-PA induction by butyrate...
article 1995
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de Bart, A.C.W. (author), Quax, P.H.A. (author), Löwik, C.W.G.M. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)
Plasmin-mediated extracellular proteolysis has been implicated in the degradation of bone in normal and pathological conditions. Normal and malignant osteoblasts can produce both tissue-type plasminogen activator (t- PA) and urokinase-type plasminogen activator (u-PA). We have used the osteosarcoma cell line MG63 to address the question of...
article 1995
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Noorman, F. (author), Braat, E.A.M. (author), Rijken, D.C. (author), TNO Preventie en Gezondheid (author)
The balance of tissue-type plasminogen activator (t-PA) production and degradation determines its concentration in blood and tissues. Disturbance of this balance may result in either increased or decreased proteolysis. In the present study, we identified the receptor systems involved in the degradation of t-PA by human monocytes/macrophages in...
article 1995
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Rompelberg, C.J.M. (author), Evertz, S.J.C.J. (author), Bruijntjes-Rozier, G.C.D.M. (author), van den Heuvel, P.D. (author), Verhagen, H. (author), TNO Voeding (author)
The influence of in vivo treatment with eugenol on established mutagens was studied to determine whether eugenol has antigenotoxic potential. The effects of eugenol in rats was investigated in the unscheduled DNA synthesis (UDS) assay with established mutagens and the Salmonella typhimurium mutagenicity assay. In addition, the effect of in vitro...
article 1996
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Hissink, A.M. (author), Oudshoorn, M.J. (author), van Ommen, B. (author), Haenen, G.R.M.M. (author), van Bladeren, P.J. (author)
The oxidative biotransformation of 1, 2-dichlorobenzene (1, 2-DCB) was investigated using hepatic microsomes from male Wistar, Fischer-344 and Sprague-Dawley (SD) rats, phenobarbital (PB)- and isoniazid (ISO) pretreated male Wistar rats and from man. In addition, microsomes from cell lines selectively expressing one cytochrome P450 (P4502E1, 1A1...
article 1996
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Quax, P.H.A. (author), Lamfers, M.L.M. (author), Grimbergen, J.M. (author), Teeling, J. (author), Hoeben, R.C. (author), van Nieuw Amerongen, G.P. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
The plasminogen activation (PA) system is involved in vascular remodelling. Modulating its activity in vascular cells might be a way to interfere in processes such as angiogenesis and restenosis. Adenoviral vectors have become a favourable tool for direct gene transfer into vascular cells. In the interest of using adenoviral vectors to modulate...
article 1996
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Arts, J. (author), Grimbergen, J. (author), Bosma, P.J. (author), Rahmsdorf, H.J. (author), Kooistra, T. (author), Gaubius Laboratory, TNO-PG, Leiden, Netherlands Institut für Genetik, Forschungszentrum Karlsruhe, Germany TNO Preventie en Gezondheid (author)
Experiments were designed to clarify the role of c-Jun/c-Fos and of putative phorbol 12-myristate-13-acetate-(PMA)-responsive elements (TREs) in the induction of plasminogen-activator inhibitor 1 (PAI-1) gene transcription in the human hepatoma cell line HepG2 by activators of protein kinase C (PKC). Treatment of HepG2 cells with the phorbol...
article 1996
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Anderson, D. (author), Yardley-Jones, A. (author), Hambly, R.J. (author), Vives-Bauza, C. (author), Smykatz-Kloss, V. (author), Chua-anusorn, W. (author), Webb, J. (author), TNO BIBRA International (author)
Thalassaemia is a group of genetic diseases where haemoglobin synthesis is impaired. This chronic anaemia leads to increased dietary iron absorption, which develops into iron overload pathology. Treatment through regular transfusions increases oxygen capacity but also provides iron through the red cells' haemoglobin. An essential treatment, in...
article 2000
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Versantvoort, C.H.M. (author), Ondrewater, R.C.A. (author), Duizer, E. (author), van de Sandt, J.J.M. (author), Gilde, A.J. (author), Groten, J.P. (author), TNO Voeding (author)
Purpose: previous studies have shown that the rat small intestinal cell line IEC-18 provides a size-selective barrier for paracellularly transported hydrophilic macromolecules. In order to determine the utility of IEC-18 cells as an in vitro model to screen the passive paracellular and transcellular components of the intestinal transport of...
article 2002
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Dommels, Y.E.M. (author), Haring, M.M.G. (author), Keestra, N.G.M. (author), Alink, G.M. (author), van Bladeren, P.J. (author), van Ommen, B. (author)
This study was conducted to investigate the role of the enzyme cyclooxygenase (COX) and its prostaglandin product PGE2 in n-6 and n-3 polyunsaturated fatty acid (PUFA)-mediated effects on cellular proliferation of two human colorectal carcinoma cell lines. The long chain PUFAs eicosapentaenoic acid (EPA; 20:5n-3) and arachidonic acid (AA; 20:4n...
article 2003
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Brink, M. (author), de Goeij, A.F.P.M. (author), Weijenberg, M.P. (author), Roemen, G.M.J.M. (author), Lentjes, M.H.F.M. (author), Pachen, M.M.M. (author), Smits, K.M. (author), de Bruïne, A.P. (author), Goldbohm, R.A. (author), van den Brandt, P.A. (author)
Activation of K-ras oncogene has been implicated in colorectal carcinogenesis, being mutated in 30-60% of the adenocarcinomas. In this study, 737 incident colorectal cancer (CRC) patients, originating from 120 852 men and women (55-69 years at baseline) participating in the Netherlands Cohort Study (NLCS), were studied in order to evaluate...
article 2003
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Faassen, F. (author), Kelder, J. (author), Lenders, J. (author), Onderwater, R. (author), Vromans, H. (author)
Purpose. The purpose of this work was to study the relevant physicochemical properties for the absorption of steroids. Methods. Various physicochemical properties of steroids were calculated (molecular weight, ClogP, static polar surface area [PSA], etc.). Within this series of steroids, different pharmacological groups were defined. Based on...
article 2003
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Stierum, R. (author), Gaspari, M. (author), Dommels, Y. (author), Ouatas, T. (author), Pluk, H. (author), Jespersen, S. (author), Vogels, J. (author), Verhoeckx, K. (author), Groten, J. (author), van Ommen, B. (author)
Here, we describe a proteomics approach to study protein expression changes in differentiating Caco-2 cells. Caco-2 is a colorectal carcinoma cell line, which upon differentiation loses its tumorigenic phenotype and displays characteristics of mature enterocytes, including brush borders with microvilli. Cells were grown in culture flasks and...
article 2003
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Oomen, A.G. (author), Tolls, J. (author), Sips, A.J.A.M. (author), Groten, J.P. (author)
Children might be exposed substantially to contaminants such as lead via soil ingestion. In risk assessment of soil contaminants there is a need for information on oral bioavailability of soilborne lead. Oral bioavailability can be seen as the result of four steps: (1) soil ingestion; (2) mobilization from soil during digestion, i.e.,...
article 2003
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Roza, L. (author), de Vogel, N. (author), van Delft, J.H.M. (author)
Hydroquinone (HQ) occurs in the environment as a result of manmade processes as well as in natural products from plants and animals. The compound has been reported to produce chromosomal effects in some in vivo and in vitro animal models. However, its potential to produce similar effects in human lymphocytes is less clear. To obtain more...
article 2003
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