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Nieuwenhuizen, W. (author), Voskuilen, M. (author), Hermans, J. (author), Gaubius instituut TNO (author)
The present study was undertaken as a step to delineate further the localization of the calcium-binding sites in fibrinogen and to assess the anticlotting properties of fibrinogen degradation products. To this purpose, fragments Y were prepared by plasmin digestion of human fibrinogen in the presence of added Ca2+, and purified. We found that,...
article 1982
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Verheijen, J.H. (author), Nieuwenhuizen, W. (author), Wijngaards, G. (author), Gaubius instituut TNO (author)
Tissue activator-mediated plasminogen activation is potentiated both by fibrin and by some soluble fibrin(ogen) fragments. The potentiating effect of the different fragments decreases in the order fibrin monomer > D-dimer > Y > D EGTA > Dcate > X. Fibrinogen and the fragments Ecate, E EGTA and E fibrin have almost no effect. The existence of a...
article 1982
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Kaptein, A. (author), Roodenburg, L. (author), Princen, H.M.G. (author), Gaubius Institute TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Addition of sodium butyrate to the culture medium of the human hepatoma cell line Hep G2 resulted in a time- and dose-dependent increase in the secretion of apolipoprotein A-I (apo A-I) and apolipoprotein B100 (apo B100). After a 24 h preincubation period, a 2.4- and 2.2-fold increase in the secretion of apo A-I and apo B100 respectively was...
article 1991
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Huisman, L.G.M. (author), Meijer, P. (author), van Griensven, J. (author), Kluft, C. (author), Gaubius Laboratory Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
t-PA depleted citrated plasma was used to prepare standards of different molecular forms of plasminogen activator inhibitor 1 (PAI-1). These standards were used to evaluate the specificity of two new PAI-1 antigen assays: the TintElize PAI-1 antigen assay (cat. no. 210221) and the Innotest PAI-1. Differences in specificity were apparent: the...
article 1992
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Meijer, P. (author), Westerveld, W. (author), Huisman, L.G.M. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)
Comparison of the results for α2-antiplasmin activity of plasma samples (n = 61, range 0-125%) obtained by the 'immediate plasmin inhibition test' (IPIT) and by the automated method for the MLA Electra 1000C of Dade® shows a good correlation (R = 0.935) but a systematic disagreement especially relevant in the range below 40%. An improved method...
article 1994
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Meijer, P. (author), Kret, R. (author), Bloetjes, P.T.M. (author), Rosen, S. (author), Kluft, C. (author), TNO Preventie en Gezondheid (author)
Blood collection and handling procedures for t-PA activity have thusfar been rather strict and have restrained the use in clinical practice. We evaluated simplifications in the procedures for specifically the use of Stabilyte® vacutainers with respect to time before centrifugation of blood, storage of plasma, times of inversion of the collection...
article 1994
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Kluft, C. (author), TNO Preventie en Gezondheid (author)
In the population at large and in individual normal volunteers, baseline molar concentratios of t-PA and PAI-1 in plasma show a strong correlation. Evidence is summarized that insulin resistance is a prominent cause of interindividual variation, next to possible contributions of vascular inflammation and steroid hormones. In healthy volunteers,...
article 1994
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Hennis, B.C. (author), Boomsma, D.I. (author), van Boheemen, P.A. (author), Engesser, L. (author), Kievit, P. (author), Dooijewaard, G. (author), Kluft, C. (author), TNO Preventie en Gezondheid (author)
A pedigree-based maximum likelihood method developed by Lange et al. (12) was used to study the contribution of a newly defined di-allelic polymorphism in histidine-rich glycoprotein (HRG) to the plasma levels of HRG. In four families (n = 99) and 20 volunteers we found a heritability of 70%, an age effect of 3% and an effect of individual...
article 1995
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van Vliet, A.K. (author), van Thiel, G.C.F. (author), Huisman, R.H. (author), Moshage, H. (author), Yap, S.H. (author), Cohen, L.H. (author), TNO Preventie en Gezondheid (author)
The three vastatins examined, lovastatin, simvastatin and pravastatin, are equally strong inhibitors of the sterol synthesis in human hepatocytes in culture with IC50-values of 4.1, 8.0 and 2.0 nM, respectively. However, in the human extrahepatic cells: umbilical vascular endothelial cells, retinal pigment epithelial cells, cornea fibroblasts...
article 1995
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Hennis, B.C. (author), van Boheemen, P.A. (author), Wakabayashi, S. (author), Koide, T. (author), Hoffmann, J.J.M.L. (author), Kievit, P. (author), Dooijewaard, G. (author), Jansen, J.G. (author), Klutf, C. (author), TNO Preventie en Gezondheid (author)
Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immune-affinity chromatography followed by chromatography with heparin-Sepharose. Both forms were single-chain molecules and the apparent molecular weights of form 1 and form 2...
article 1995
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de Maat, M.P.M. (author), de Knijff, P. (author), Green, F.R. (author), Thomas, A.E. (author), Jespersen, J. (author), Kluft, C. (author), TNO Preventie en Gezondheid (author)
Elevated plasma fibrinogen levels represent an increased risk for cardiovascular diseased, but the mechanism explaining this association is still not clear. Genetic differences may play a role, because it has been shown that individuals who carry the rare alleles of polymorphisms in the genes for the Bβ-chain (Bcl I and G/A 455) and the Aα-chain...
article 1995
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de Rijke, Y.B. (author), Verwey, H.F. (author), Vogelezang, C.J.M. (author), van der Velde, E.A. (author), Princen, H.M.G. (author), van der Laarse, A. (author), Bruschke, A.V.G. (author), van Berkel, T.J.C. (author), Gaubius Instituut TNO (author)
Oxidation of low-density lipoprotein (LDL) may play a causal role in atherosclerosis. In this study we analyzed whether the severity of progression of coronary atherosclerosis is related to the susceptibility of LDL to oxidative modification. On the basis of repeated coronary angiography, 28 coronary bypass patients were divided into two groups:...
article 1995
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Draijer, R. (author), Atsma, D.E. (author), van der Laarse, A. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Previous studies have demonstrated that cGMP and cAMP reduce the endothelial permeability for fluids and macromolecules when the endothelial permeability is increased by thrombin. In this study, we have investigated the mechanism by which cGMP improves the endothelial barrier function and examined whether nitric oxide (NO) can serve as an...
article 1995
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Haider, A.W. (author), Andreotti, F. (author), Hackett, D.R. (author), Tousoulis, D. (author), Kluft, C. (author), Maseri, A. (author), Davies, G.J. (author), Gaubius Instituut TNO (author)
Objectives. This study investigated the influence of early spontaneous intermittent reperfusion on the extent of myocardial damage and its relation to endogenous hemostatic activity, Background. In the early phase of acute myocardial infarction coronary occlusion is often intermittent, even before thrombolytic therapy is administered. The...
article 1995
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Arts, J. (author), Lansink, T. (author), Grimbergen, J. (author), Toet, K.H. (author), Kooistra, T. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
We have previously shown that the pleiotropic agent sodium butyrate strongly stimulates tissue-type plasminogen activator (t-PA) expression in human umbilical vein endothelial cells (HUVEC). Here we provide the following evidence that the butyrate-induced t-PA expression in HUVEC involves histone H4 acetylation. (1) t-PA induction by butyrate...
article 1995
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Draijer, R. (author), Vaandrager, A.B. (author), Nolte, C. (author), de Jonge, H.R. (author), Walter, U. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Previous studies demonstrated that the thrombin-induced permeability of endothelial cell monolayers is reduced by the elevation of cGMP. In the present study, the presence of cGMP-dependent protein kinase (cGMP-PK) immunoreactivity and activity in various types of human endothelial cells (ECs) and the role of cGMP-PK in the reduction of thrombin...
article 1995
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Myrup, B. (author), Hansen, P.M. (author), Jensen, T. (author), Kofoed-Enevoldsen, A. (author), Feldt-Rasmussen, B. (author), Gram, J. (author), Kluft, C. (author), Jespersen, J. (author), Deckert, T. (author), Gaubius Instituut TNO (author)
We investigated the effect of heparin on urinary albumin excretion in patients with insulin-dependent diabetes mellitus. 39 patients with persistent urinary albumin excretion of 30-300 mg/24 h were randomly treated for 3 months with subcutaneous injections twice daily of isotonic saline, 5000 IU unfractionated heparin, or 2000 anti-Xa IU low...
article 1995
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Theuns, H.M. (author), TNO Preventie en Gezondheid (author)
In this article the importance of mineral suppletion for teeth and jawbone is explained in a step-wise manner. The steps discussed are the pre-eruptive phase of teeth and the growth, adult and ageing phase of the skeleton. Mineral suppletion should be seen as a measure to prevent dental caries (fluoride) and to ensure a maximal resistance...
article 1995
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Noorman, F. (author), Braat, E.A.M. (author), Rijken, D.C. (author), TNO Preventie en Gezondheid (author)
The balance of tissue-type plasminogen activator (t-PA) production and degradation determines its concentration in blood and tissues. Disturbance of this balance may result in either increased or decreased proteolysis. In the present study, we identified the receptor systems involved in the degradation of t-PA by human monocytes/macrophages in...
article 1995
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Mestecky, J. (author), Hamilton, R.G. (author), Magnusson, C.G.M. (author), Jefferis, R. (author), Vaerman, J.P. (author), Goodall, M. (author), de Lange, G.G. (author), Moro, I. (author), Aucouturier, P. (author), Radl, J. (author), Cambiaso, C. (author), Silvain, C. (author), Preud'homme, J.L. (author), Kusama, K. (author), Carlone, G.M. (author), Biewenga, J. (author), Kobayashi, K. (author), Skvaril, F. (author), Reimer, C.B. (author), Gaubius Instituut TNO (author)
51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme...
article 1996
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