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Padró, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
The aim of the present study was to investigate whether hypothyroidism would affect the components of the rat plasma fibrinolytic system. Hypothyroidism was induced by feeding rats a thiouracil-containing diet for 2 weeks. During this period, half of the animals received subcutaneous injections of L-thyroxine (20 μg/kg daily) to restore thyroid...
article 1993
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van Hinsbergh, V.W.M. (author), van den Berg, E.A. (author), Fiers, W. (author), Dooijewaard, G. (author), Gaubius Instituut TNO (author)
Endothelial cells play an important role in the regulation of fibrinolysis by the production of several key regulatory proteins. The cytokines tumor necrosis factor (TNF), lymphotoxin, and interleukin-1 (IL-1), but not interleukin-6, increase the production of plasminogen activator inhibitor-1 (PAI-1) by endothelial cells, whereas they have no...
article 1990
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van Hinsbergh, V.W.M. (author), Bauer, K.A. (author), Kooistra, T. (author), Kluft, C. (author), Dooijewaard, G. (author), Sherman, M.L. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)
Several investigators have reported that tumor necrosis factor (TNF) can alter the production of plasminogen activator type-1 (PAI-1) and plasminogen activators (PAs) by endothelial cells in vitro. We have examined the in vivo effects of recombinant human TNF administration on fibrinolysis as assessed by parameters in plasma during a 24-hour...
article 1990
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de Maat, M.P.M. (author), de Bart, A.C.W. (author), Hennis, B.C. (author), Meijer, P. (author), Havelaar, A.C. (author), Mulder, P.G.H. (author), Kluft, C. (author), Gaubius Instituut TNO (author)
We compared intraindividual and interindividual variability in the plasma levels of fibrinogen, tissue-type plasminogen activator (TPA) antigen, plasminogen activator inhibitor (PAI) activity, and C-reactive protein (CRP) in 20 healthy, young individuals and 26 patients with stable angina pectoris (AP) who were at higher risk for cardiovascular...
article 1996
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Velthuis-te Wierik, E.J.M. (author), Meijer, P. (author), Kluft, C. (author), van den Berg, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Impaired fibrinolytic activity has been reported in the elderly and is thought to play a role in the etiology of cardiovascular disease, one of the leading causes of death in most Western countries. Since restriction of energy intake has been demonstrated to act beneficially on the aging process in a variety of species, we studied the effect of...
article 1995
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van Hinsbergh, V.W.M. (author), Sprengers, E.D. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)
Human foreskin microvascular endothelial cells synthesize and release tissue-type plasminogen activator (t-PA) in similar amounts as do endothelial cells from umbilical cord artery and vein. Human thrombin increases the production of t-PA by these cells, which could be visualized from 8 h after addition of 0.1-5 units/ml thrombin by fibrin...
article 1987
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D'Angelo, A. (author), Kluft, C. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
In nine patients with non-malignant diseases undergoing major upper abdominal surgery, the mechanism of the postoperative fibrinolytic shut-down was investigated because of its potential significance for postoperative deep vein thrombosis by employing new and specific methods for assessing and stimulating the fibrinolytic system. The shut-down...
article 1985
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van Hinsbergh, V.W.M. (author), Kooistra, T. (author), van den Berg, E.A. (author), Princen, H.M.G. (author), Fiers, W. (author), Emeis, J.J. (author), Gaubius instituut TNO (author)
The vascular endothelium plays an important role in fibrinolysis by producing tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI). The monokine tumor necrosis factor (human recombinant TNF) increased the production of PAI by cultured human endothelial cells from umbilical vein (twofold) and from foreskin...
article 1988
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Verheijen, J.H. (author), Chang, G.T.G. (author), Kluft, C. (author), Gaubius Instituut TNO (author)
Human plasma contains a fast-acting t-PA inhibitor, which is not identical with ??2-antiplasmin or ??2-macroglobulin. The concentration of this inhibitor in normal plasma is highly variable, much lower than that of known plasma protease inhibitors, and in the range of physiologically occurring plasma concentrations of t-PA (0-2 IU/ml). The...
article 1984
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Engesser, L. (author), Brommer, E.J.P. (author), Kluft, C. (author), Briet, E. (author), Gaubius Instituut TNO (author)
Two hundred and three patients with venous thrombophilia were investigated in order to find out whether an elevated plasma concentration of plasminogen activator inhibitor (PAI) could be a cause of their tendency to thrombosis. The patients were studied in an asymptomatic period about 3 months after their last thromboembolic episode. PAI...
article 1989
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Brommer, E.J.P. (author), Derkx, F.H.M. (author), Schalekamp, M.A.D.H. (author), Dooijewaard, G. (author), Klaauw, V.D.M.M. (author), Gaubius instituut TNO (author)
In eight male patients with normal liver and kidney function fibrinolytic components were measured in arterial blood and in renal and hepatic vein blood, obtained during catheterization for analysis of hypertension. Blood samples were collected simultaneously from veins und corresponding arteries before and 5 minutes after the completion of...
article 1988
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de Bart, A.C.W. (author), Quax, P.H.A. (author), Löwik, C.W.G.M. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)
Plasmin-mediated extracellular proteolysis has been implicated in the degradation of bone in normal and pathological conditions. Normal and malignant osteoblasts can produce both tissue-type plasminogen activator (t- PA) and urokinase-type plasminogen activator (u-PA). We have used the osteosarcoma cell line MG63 to address the question of...
article 1995
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van Hinsbergh, V.W.M. (author), Kooistra, T. (author), Scheffer, M.A. (author), van Bockel, J.H. (author), van Muijen, G.N.P. (author), Gaubius Instituut TNO (author)
It has been reported that omental fat tissue is a good source of human microvascular endothelial cells. By characterization we demonstrate that the epithelioid cells isolated from omental tissue are not endothelial cells, but mesothelial cells. They contain abundant cytokeratins 8 and 18, which are absent in endothelial cells, and vimentin. No...
article 1990
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van Hinsbergh, V.W.M. (author), Bertina, R.M. (author), van Wijngaarden, A. (author), van Tilburg, N.H. (author), Emeis, J.J. (author), Haverkate, F. (author), Gaubius instituut TNO (author)
Confluent cultures of endothelial cells from human umbilical cord were used to study the effect of activated human protein C (APC) on the production of plasminogen activators, plasminogen activator-inhibitor, and factor VIII-related antigen. Addition of APC to the cells in a serum-free medium did not affect the production of tissue-type...
article 1985
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Emeis, J.J. (author), Edgell, C.J.S. (author), Gaubius instituut TNO (author)
The fibrinolytic characteristics of the endothelial hybrid cell line EA.hy 926, established by fusing a human umbilical vein endothelial cell with a human carcinoma cell line, were studied. The hybrid cell line produced large amounts of tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor type 1, and a small amount of...
article 1988
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Rijken, D.C. (author), Wijngaards, G. (author), Zaal-de Jong, M. (author), Welbergen, J. (author), Gaubius instituut TNO (author)
A procedure was developed for the purification of a plasminogen activator from human uterine tissue. It involves six consecutive steps: extraction of the plasminogen activator from delipidated uterine tissue with 0.3 M potassium acetate buffer, pH 4.2; ammonium sulphate precipitation; zinc chelate-agarose chromatography; n-butyl-agarose...
article 1979
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Kooistra, T. (author), Opdenberg, J.P. (author), Toet, K. (author), Hendriks, H.F.J. (author), van den Hoogen, R.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
Tissue-type plasminogen activator (t-PA) and its inhibitor, plasminogen activator inhibitor 1 (PAI-1), play an important role in regulating the fibrinolytic capacity of plasma. Both t-PA and PAI-1 are synthesized by the endothelium. We report that retinoic acid (vitamin A acid) and other retinoids rather specifically stimulate the production of...
article 1991
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Sprengers, E.D. (author), Kluft, C. (author), Gaubius Instituut TNO (author)
Chemicals/CAS: fibrin, 9001-31-4; plasminogen activator inhibitor, 105844-41-5; plasminogen activator, 9039-53-6; Carrier Proteins; Glycoproteins; Plasminogen Activators, EC 3.4.21.-; Plasminogen Inactivators; Protease Inhibitors; protease-nexin
article 1987
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Emeis, J.J. (author), van Houwelingen, A.C. (author), van den Hoogen, C.M. (author), Hornstra, G. (author), Gaubius Institute TNO, 2300 AP Leiden, Netherlands (author)
For a period of 6 weeks, 76 healthy male volunteers consumed during their daily main meal the contents of one tin (~ 135 g) of either fish (mackerel) paste or meat paste. Fibrinolytic parameters were determined in plasma samples obtained at the beginning and at the end of the experimental period. No changes were found in plasminogen, α2...
article 1989
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Padró, T. (author), van den Hoogen, C.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)
Tissue-type plasminogen activator (PA) activity and antigen was measured in nine different tissues from healthy rats (brain, lung, heart, liver, spleen, kidney, adrenal, aorta and skeletal muscle). After extraction in a KSCN buffer (for kidney) or in an acid acetate buffer (for all other tissues), total PA activity was determined by an improved...
article 1990
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