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Coulier, L. (author), Bas, R. (author), Jespersen, S. (author), Verheij, E. (author), van der Werf, M.J. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven TNO Voeding (author)
We have developed an analytical method, consisting of ion-pair liquid chromatography coupled to electrospray ionization mass spectrometry (IP-LC-ESI-MS), for the simultaneous quantitative analysis of several key classes of polar metabolites, like nucleotides, coenzyme A esters, sugar nucleotides, and sugar bisphosphates. The use of the ion-pair...
article 2006
Mol, H.G.J. (author), van Dam, R.C.J. (author), Vreeken, R.J. (author), Steijger, O.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
A straightforward and reliable method was developed for the determination of chlormequat in pears by liquid chromatography/mass spectrometry (LC/MS). Water and methanol were compared as extraction solvents. Because no significant differences in extraction efficiency or repeatability were found, water was chosen as the extraction solvent. The...
article 2000
Jónasdóttir, H.S. (author), Ioan-Facsinay, A. (author), Kwekkeboom, J. (author), Brouwers, H. (author), Zuurmond, A.M. (author), Toes, R. (author), Deelder, A.M. (author), Giera, M. (author)
Here we present an advanced platform for the analysis of specialized pro-resolving mediators (SPM), including a set of pro-inflammatory lipids and respective biochemical pathway markers. The platform is characterized by a largely simplified sample preparation protocol, employing methanol protein-precipitation instead of solid phase extraction....
article 2014