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van Hemmen, J.J. (author), Meuling, W.J.A. (author), Bleichrodt, J.F. (author), Medisch Biologisch Laboratorium TNO (author)
Paranitroacetophenone (PNAP) and triacetoneamine N oxyl (TAN), which sensitize anoxic cells to ionizing radiation, do not increase the radiosensitivity of bacteriophage DNA irradiated in the dry state, showing that the 'direct action model' of sensitization does not hold in this experimental set up. PNAP and TAN added within 1.5 msec after...
article 1973
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Vos, O. (author), Budke, L. (author), Grant, G.A. (author), Medisch Biologisch Laboratorium TNO (author)
In tissue culture, protection against X irradiation by a number of cysteamine derivatives was studied and the results were compared with data obtained in mice. Compounds with a covered SH group, like WR 638, cysteamine phosphate, WR 2721 and AE AE 48527, showed practically no protection when dissolved in tissue culture medium, but developed a...
article 1976
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Mattern, I.E. (author), Medisch Biologisch Laboratorium RVO-TNO (author)
article 1976
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Vos, O. (author), Grant, G.A. (author), Budke, L. (author), Medisch Biologisch Laboratorium RVO-TNO (author)
The effect of diamide on the radiosensitivity of T cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0.1, 0.5 and 1.0 mM diamide produced dose modifying factors of 0.81, 0.60 and 0.55,...
article 1976
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Hooisma, J. (author), Slaaf, D.W. (author), Meeter, E. (author), Stevens, W.F. (author), Medisch Biologisch Laboratorium TNO (author)
The influence of various neural explants on the morphology and the survival of chick muscle fibers was studied. A method was developed to evaluate the condition of the muscle fibers using the following four morphological parameters: cross striation, thickness, number of fibers and absence of vacuoles. Chick as well as mouse spinal cord explants...
article 1978
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Wade, D.R. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)
Chemicals/CAS: hydrazine, 10217-52-4, 13775-80-9, 18500-32-8, 302-01-2, 7803-57-8; isoniazid, 54-85-3, 62229-51-0, 65979-32-0; manganese, 16397-91-4, 7439-96-5
article 1980
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Hooisma, J. (author), Dam, E.W. (author), Medisch Biologisch Laboratorium TNO (author)
Chemicals/CAS: 2 hexanone, 591-78-6; acetonylacetone, 110-13-4; hexane, 110-54-3
article 1981
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Magchielse, T. (author), Hooisma, J. (author), Muijser, H. (author), Medisch Biologisch Laboratorium TNO (author)
The measurement of passive electrical membrane properties in cultured muscle cells is complicated by the fact that the electrotonic length in these cells often exceeds their actual length, leading to serious deviations from the simple infinite cable behaviour. By using very short current pulses for these measurements and integrating the...
article 1981
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Schellekens, H. (author), Weimar, W. (author), de Reus, A. (author), Bolhuis, R.L.H. (author), Cantell, K. (author), Instituut voor Experimentele Gerontologie TNO (author)
Chemicals/CAS: Adjuvants, Immunologic; Antibodies, Viral; Interferons, 9008-11-1
article 1981
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Klein, J.C. (author), Radiobiologisch Instituut TNO (author)
In vitro assays that permit cloning of tumour cells in soft agar have been improved during the last 5 years. Two of them are claimed to be useful as test systems for the screening of new anticancer drugs and even for drug sensitivity testing of individual human tumours in devising individualized cancer chemotherapy regimens. Three assays were...
article 1981
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Creemers, P. (author), Radiobiologisch Instituut TNO (author)
Sera from normal mice and mice bearing murine mammary tumor virus (MTV)-induced tumors showed cytotoxic reactions to the MTV-producing Mm5mt/cl cell line. The reaction could be blocked by the addition of MTV, but not of purified gp52 and p28. In sera from tumor bearers, cytotoxic responses ranged from 15 to 66%; reactivity was generally highest...
article 1981
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Hooisma, J. (author), de Krijger, J. (author), de Groot, D.M.G. (author), Medisch Biologisch Laboratorium TNO (author)
Previously tropic effects of extracts from whole chick embryos and from innervated muscles on cultured muscle cells were described. The present study demonstrated similar effects of extracts from 10-days denervated chick muscles. Extracts from innervated as well as from denervated muscles exsanguinated in vivo with saline prior to dissection...
article 1981
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Lubbe, F.H. (author), Hooijkaas, H. (author), Preesman, A.A. (author), Medisch Biologisch Laboratorium TNO (author)
The effect of X-irradiation upon murine antibody-forming cell precursors (B cells) was established in cultures of spleen cells stimulated with the B cell mitogen lipopolysaccharide (LPS). At day 5 and 7 the numbers of IgM- and IgG2-secreting cells were determined in cultures of irradiated and nonirradiated spleen cells. From these numbers a D(o)...
article 1982
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Gaubius instituut TNO (author), Kempen, H.J.M. (author), van Vos-Holstein, P.M. (author), de Lange, J. (author)
Contents of bile acids and lipids, as well as rates of triglyceride synthesis, were determined in isolated hepatocytes from control or cholestyramine-fed rats (denoted below as "control" or "treated" hepatocytes, respectively). During a 3-hr incubation period, total bile acid production was markedly higher in "treated" cells than in "conrol"...
article 1982
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Rutten, A.A.J.J.L. (author), Wilmer, J.W.G.M. (author), Instituut CIVO-Toxicologie en voeding TNO (author)
article 1986
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van Hinsbergh, V.W.M. (author), Kooistra, T. (author), van den Berg, E.A. (author), Princen, H.M.G. (author), Fiers, W. (author), Emeis, J.J. (author), Gaubius instituut TNO (author)
The vascular endothelium plays an important role in fibrinolysis by producing tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI). The monokine tumor necrosis factor (human recombinant TNF) increased the production of PAI by cultured human endothelial cells from umbilical vein (twofold) and from foreskin...
article 1988
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Emeis, J.J. (author), Edgell, C.J.S. (author), Gaubius instituut TNO (author)
The fibrinolytic characteristics of the endothelial hybrid cell line EA.hy 926, established by fusing a human umbilical vein endothelial cell with a human carcinoma cell line, were studied. The hybrid cell line produced large amounts of tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor type 1, and a small amount of...
article 1988
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Medisch Biologisch Laboratorium TNO (author), Mol, M.A.E. (author), van de Ruit, A.M.B.C. (author), Kluivers, A.W. (author)
In cultured human epidermal cells exposure to the vesicant sulfur mustard (HD) causes a decrease of the NAD+ content, which depends on the dose and the time period between exposure to HD and NAD+ measurement. Presumably, this NAD+ loss is due to activation of the enzyme NAD:protein ADP-ribosyltransferase (ADPRT) and may lead to glycolysis...
article 1989
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Mol, M.A.E. (author), de Vries-Van de Ruit, A.M.B.C. (author), Medisch Biologisch Laboratorium TNO (author)
The concentration- and time-dependent effects of sulphur mustard on cultured human epidermal keratinocyte function were investigated with respect to cell proliferation, DNA and protein synthesis, the level of NAD+ and ATP, and the intracellular activity of lactate dehydrogenase. If human epidermal keratinocytes were stimulated to proliferate by...
article 1992
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Gouka, R.J. (author), Stam, H. (author), Fellinger, A.J. (author), Muijsenberg, R.J.G.T. (author), van de Wijngaard, A.J. (author), Punt, P.J. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)
In this study, induction and repression kinetics of the expression of the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene under defined physiological conditions was analyzed at the mRNA and the protein levels. Induction was analyzed by pulsing D-xylose to a sucrose-limited continuous culture of an A. awamori 1,4-β-endoxylanase A (EXLA)...
article 1996
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