Searched for:
(1 - 20 of 40)

Pages

document
Kastelein, R.A. (author), Helder-Hoek, L. (author), Cornelisse, S.A. (author), von Benda-Beckmann, A.M. (author), Lam, F.P.A. (author), de Jong, C.A.F. (author), Ketten, D.R. (author)
Noise-induced temporary hearing threshold shift (TTS) was studied in a harbor porpoise exposed to impulsive sounds of scaled-down airguns while both stationary and free-swimming for up to 90 min. In a previous study, ∼4 dB TTS was elicited in this porpoise, but despite 8 dB higher single-shot and cumulative exposure levels (up to 199 dB re 1...
article 2020
document
Harb, H. (author), van Tol, E.A.F. (author), Heine, H. (author), Braaksma, M. (author), Gross, G. (author), Overkamp, K. (author), Hennen, M. (author), Alrifai, M. (author), Conrad, M.L. (author), Renz, H. (author), Garn, H. (author)
Background: Oral supplementation with probiotic bacteria can protect against the development of allergic and inflammatory diseases. Objective: The aim of this study was to investigate potential immunomodulatory and allergy-protective effects of processed Lactobacillus rhamnosus GG (LGG)-derived supernatants early in life in neonatal mice....
article 2013
document
van Duuren, J.B.J.H. (author), Wijte, D. (author), Leprince, A. (author), Karge, B. (author), Puchalka, J. (author), Wery, J. (author), Dos Santos, V.A.P.M. (author), Eggink, G. (author), Mars, A.E. (author)
Pseudomonas putida KT2440-JD1 was derived from P. putida KT2440 after N-methyl-N'-nitro-N-nitrosoguanidine (NTG)-mutagenesis and exposure to 3-fluorobenzoate (3-FB). The mutant was no longer able to grow using benzoate as a sole carbon source, but co-metabolized benzoate to cis, cis-muconate during growth on glucose, which accumulated in the...
article 2011
document
Joosen, M.J.A. (author), van der Schans, M.J. (author), van Dijk, C.G.M. (author), Kuijpers, W.C. (author), Wortelboer, H.M. (author), van Helden, H.P.M. (author)
One of the shortcomings of current treatment of nerve agent poisoning is that oximes hardly penetrate the blood-brain barrier (BBB), whereas nerve agents easily do. Increasing the concentration of oximes in the brain, would therefore provide an attractive approach to improve medical countermeasures. An explanation for limited penetration might...
article 2011
document
Wijte, D. (author), van Baar, B.L.M. (author), Heck, A.J.R. (author), Altelaar, A.F.M. (author)
To enhance target production from biocatalysts, it is necessary to thoroughly understand the molecular mechanisms involved in production, degradation, and, importantly, adaptation to the required environment. One such bacterium with high potential for biocatalysis is the solvent-tolerant bacteria Pseudomonas putida S12, which, among others, is...
article 2011
document
Molhoek, E.M. (author), van Dijk, A. (author), Veldhuizen, E.J.A. (author), Haagsman, H.P. (author), Bikker, F.J. (author)
A truncated version of host defense peptide chicken cathelicidin-2, C1-15, possesses potent, broad spectrum antibacterial activity. A variant of this peptide, F2,5,12W, which contains 3 phenylalanine to tryptophan substitutions, possesses improved antibacterial activity and lipopolysaccharide (LPS) neutralizing activity compared to C1-15. In...
article 2011
document
Kaman, W.E. (author), Wolterink, A.F.W.M. (author), Bader, M. (author), Boele, L.C.L. (author), van der Kleij, D. (author), TNO Defensie en Veiligheid (author)
The endogenous danger signal bradykinin was recently found implicated in the development of immunity against parasites via dendritic cells. We here report an essential role of the B2 (B2R) bradykinin receptor in the early immune response against Listeria infection. Mice deficient in B2R (B2R-/- mice) were shown to suffer from increased hepatic...
article 2009
document
Noort, D. (author), Fidder, A. (author), Degenhardt-Langelaan, C.E.A.M. (author), Hulst, A.G. (author), TNO Defensie en Veiligheid (author)
The persistence in rats of sulfur mustard adducts to albumin and hemoglobin was studied in vivo after exposure (intravenously; 0.3 mg/kg; approximately 0.1 LD50) of rats to sulfur mustard. The albumin adduct (S-HETE)Cys-Pro-Tyr was detectable up to 7 days after the exposure, while the adduct to the N-terminal valine in hemoglobin was still...
article 2008
document
Bobeldijk, I. (author), Hekman, M. (author), van der Vries de- Weij, J. (author), Coulier, L. (author), Ramaker, R. (author), Kleemann, R. (author), Kooistra, T. (author), Rubingh, C. (author), Freidig, A. (author), Verheij, E. (author), TNO Kwaliteit van Leven (author)
We report a sensitive, generic method for quantitative profiling of bile acids and other endogenous metabolites in small quantities of various biological fluids and tissues. The method is based on a straightforward sample preparation, separation by reversed-phase high performance liquid-chromatography mass spectrometry (HPLC-MS) and electrospray...
article 2008
document
Frost-Christensen, L.N. (author), Mastbergen, S.C. (author), Vianen, M.E. (author), Hartog, A. (author), de Groot, J. (author), Voorhout, G. (author), van Wees, A.M.C. (author), Lafeber, F.P.J.G. (author), Hazewinkel, H.A.W. (author), TNO Kwaliteit van Leven (author)
Objective: The most used model for joint instability is the canine anterior cruciate ligament transection (ACLT)-model. The ACLT-model can be extended with a medial meniscectomy (MX) (i.e., ACLT-MX-model) to avoid unintentional, and with that variable, meniscal damage. The present study compares the ACLT-MX-model with the more recently...
article 2008
document
Hu, C. (author), van Dommelen, J. (author), van der Heijden, R. (author), Spijksma, G. (author), Reijmers, T.H. (author), Wang, M. (author), Slee, E. (author), Lu, X. (author), Xu, G. (author), van der Greef, J. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven (author)
A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometry method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C<sub>8</sub> column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single...
article 2008
document
Damveld, R.A. (author), Franken, A. (author), Arentshorst, M. (author), Punt, P.J. (author), Klis, F.M. (author), van den Hondel, C.A.M.J.J. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven (author)
To identify cell wall biosynthetic genes in filamentous fungi and thus potential targets for the discovery of new antifungals, we developed a novel screening method for cell wall mutants. It is based on our earlier observation that the Aspergillus niger agsA gene, which encodes a putative a-glucan synthase, is strongly induced in response to...
article 2008
document
Tomaso, H. (author), Jacobs, D. (author), Eickhoff, M. (author), Scholz, H.C. (author), al Dahouk, S. (author), Kattar, M.M. (author), Reischl, U. (author), Plicka, H. (author), Strand Olsen, J. (author), Nikkari, S. (author), Matero, P. (author), Beuret, C. (author), Ciammaruconi, A. (author), Lista, F. (author), Gala, J.-L. (author), Broll, H. (author), Appel, B. (author), Sellek Cano, R.E. (author), Ybarra de Villavicencio, M.D.C. (author), Broekhuijsen, M.P. (author), Indra, A. (author), Petersen, R. (author), Neubauer, H. (author)
Background: Yersinia pestis (Y. pestis) is a zoonotic bacterium mainly circulating among rodents and their fleas. Transmission to humans can cause bubonic, pneumonic or septicemic plague with a high case-fatality rate. Therefore, rapid and reliable diagnostic tools are crucial. The objective of this study was to assess the inter-laboratory...
article 2008
document
Intema, F. (author), de Groot, J. (author), Elshof, B. (author), Vianen, M.E. (author), Yocum, S. (author), Zuurmond, A. (author), Mastbergen, S.C. (author), Lafeber, F.P. (author), TNO Kwaliteit van Leven (author)
In studies aimed at local treatment of experimental osteoarthritis (OA) it is optimal to have an internal (untreated) OA control. Such an approach excludes interanimal variation, and allows paired statistical evaluation of treatment efficacy. For this purpose, we developed and characterized a bilateral version of the canine Groove model. We...
article 2008
document
TNO Kwaliteit van Leven (author), Kort, R. (author), Keijser, B.J. (author), Caspers, M.P.M. (author), Schuren, F.H. (author), Montijn, R. (author)
Background: In bacteriology, the ability to grow in selective media and to form colonies on nutrient agar plates is routinely used as a retrospective criterion for the detection of living bacteria. However, the utilization of indicators for bacterial viability-such as the presence of specific transcripts or membrane integrity-would overcome bias...
article 2008
document
TNO Kwaliteit van Leven (author), Bastiaansen-Jenniskens, Y.M. (author), Koevoet, W. (author), de Bart, A.C.W. (author), Zuurmond, A.M. (author), Bank, R.A. (author), Verhaar, J.A.N. (author), de Groot, J. (author), van Osch, G.J.V.M. (author)
Transforming growth factor beta (TGFbeta) is often used in cartilage tissue engineering to increase matrix formation by cells with various phenotypes. However, adverse effects of TGFbeta, such as extensive cross-linking in cultured fibroblasts, have also been reported. Our goal was to study effects of TGFbeta on collagen cross-linking and...
article 2008
document
Bikker, F.J. (author), Mars-Groenendijk, R.H. (author), Noort, D. (author), Fidder, A. (author), van der Schans, G.P. (author), TNO Defensie en Veiligheid (author)
As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time-consuming...
article 2007
document
Smither, S.J. (author), Hill, J. (author), van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Titball, R.W. (author), TNO Defensie en Veiligheid (author)
The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through...
article 2007
document
Zacharowski, K. (author), Zacharowski, P.A. (author), Friedl, P. (author), Mastan, P. (author), Koch, A. (author), Boehm, O. (author), Rother, R.P. (author), Reingruber, S. (author), Henning, R. (author), Emeis, J.J. (author), Petzelbauer, P. (author), TNO Kwaliteit van Leven (author)
Many compounds have been shown to prevent reperfusion injury in various animal models, although to date, translation into clinic has revealed several obstacles. Therefore, the National Heart, Lung, and Blood Institute convened a working group to discuss reasons for such failure. As a result, the concept of adequately powered, blinded, randomized...
article 2007
document
Bikker, F.J. (author), Kaman-van Zanten, W.E. (author), de Vries-van de Ruit, A.M.B.C. (author), Voskamp-Visser, I. (author), van Hooft, P.A.V. (author), Mars-Groenendijk, R.H. (author), de Visser, P.C. (author), Noort, D. (author), TNO Defensie en Veiligheid (author)
Drosocin is a 19-mer, cationic antimicrobial peptide from Drosophila melanogaster. The aim of the study was to examine the antibacterial spectrum of unglycosylated drosocin analogues. Furthermore, the amino acid sequence of DnaK, drosocin's intracellular target, from susceptible species was aligned and studied for sequence homology. From this a...
article 2006
Searched for:
(1 - 20 of 40)

Pages