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Wortelboer, H.M. (author), de Kruif, C.A. (author), de Boer, W.I. (author), van Iersel, A.A.J. (author), Falke, H.E. (author), Blaauboer, B.J. (author)
Changes in cytochrome P-450 isoenzymes were studied in rat liver and in primary cultures of rat hepatocytes after treatment with compounds belonging to various classes of inducers, including phenobarbital (PB), beta-naphthoflavone (BNF), and clofibrate/clofibric acid (CLOF/CLOFA). The enzyme activity toward specific substrates was measured, and...
article 1987
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Instituut CIVO-Toxicologie en voeding TNO (author), Joosse, J. (author), van Elk, R. (author), MOsselman, S. (author), Wortelboer, H.M. (author), van Diepen, J.C. (author)
The schistosome parasite, Trichobilharzia ocellata, nearly completely inhibits the reproductive activity of its intermediate host, Lymnaea stagnalis. The synthetic activity of albumen glands of infected snails at day 35 postinfection (p.i.) is only 1% of the control value. The parasite acts by humoral means. We tested the hypothesis that (a)...
article 1988
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Centraal Instituut voor Voedingsonderzoek TNO (author), Gille, J.J.P. (author), Wortelboer, H.M. (author), Joenje, H. (author)
The effect of increased intracellular oxygen activation on cellular antioxidant defenses in CHO and HeLa cells was studied. In both cell types, hyperoxic exposure (up to 4 days, 600-700 mm Hg O2) and in CHO cells menadione (up to 3 days, 15 microM) failed to affect the enzymatic antioxidant defenses Mn-containing superoxide dismutase (Mn-SOD),...
article 1988
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Instituut voor Toxicologie en Voeding TNO (author), Blaauboer, B.J. (author), Wortelboer, H.M. (author), Mennes, W.C. (author)
In vitro techniques can be applied to obtain insight into the organotoxic potential of compounds and in the underlying mechanisms of action. During the last few decades the applicability of these techniques in toxicology has been demonstrated for an increasing number of cell types. Cultured hepatocytes have been shown to provide convenient in...
article 1990
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Wortelboer, H.M. (author), de Kruyf, C.A. (author), van Iersel, A.A.J. (author), Falke, H.E. (author), Noordhoek, J. (author), Blaauboer, B.J. (author)
Changes in the isoenzyme pattern of cytochrome P450 during culture were investigated in primary cultures of rat hepatocytes, measuring specific enzyme activities in microsomes prepared from cultured cells as well as in intact monolayers. Assays of 7-ethoxyresorufin O-deethylation (EROD), 7-pentoxyresorufin O-depentylation (PROD), aniline 4...
article 1990
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Centraal Instituut voor Voedingsonderzoek TNO (author), Wortelboer, H.M. (author), de Kruif, C.A. (author), van Iersel, A.A.J. (author), Falke, H.E. (author), Noordhoek, J. (author), Blaauboer, B.J. (author)
The metabolic profile of seven subfamilies of cytochrome P450 (P450IA, IIA, IIB, IIC, IIE, IIIA, IVA) was studied in rat liver (in vivo) and in primary hepatocyte cultures (in vitro) after treatment with various inducers. The dealkylation of 7-ethoxyresorufin (EROD) and 7-pentoxyresorufin (PROD), aniline 4-hydroxylation and the regio- and...
article 1991
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Centraal Instituut voor Voedingsonderzoek TNO (author), Wortelboer, H.M. (author)
doctoral thesis 1991
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Instituut voor Voedingsmiddelentechnologie TNO (author), Wortelboer, H.M. (author), de Kruif, C.A. (author), van Iersel, A.A.J. (author), Noordhoek, J. (author), Blaauboer, B.J. (author), van Bladeren, P.J. (author), Falke, H.E. (author)
Male Wistar rats were given semi-synthetic diets supplemented with 0, 2.5, 5 and 20% cooked Brussels sprouts for 2, 7, 14 or 28 days. The effects on several cytochrome P-450 enzymes and phase II enzymes (glutathione S-transferase (GST), glucuronyl transferases 1 and 2 (GT1 and GT2) and DT-diaphorase (DTD)) in the liver and small intestinal...
article 1992
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Instituut voor Toxicologie en Voeding TNO (author), Wortelboer, H.M. (author), de Kruif, C.A. (author), van Iersela, A.A.J. (author), Falke, H.E. (author), Noordhoek, J. (author), Blaauboer, B.J. (author)
The effects of three acid condensation products of indole-3-carbinol (I3C), i.e. 3,3'-diindolylmethane (DIM), 5,6,11,12,17,18-hexahydrocyclonona[1,2-b:4,5-b:7,8-b]tri-indole (CTI) and 2,3-bis[3-indolylmethyl]indole (BII), on cytochrome P450 and phase II enzymes were studied in primary cultures of rat and cynomolgus monkey liver cells. In rat...
article 1992
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Instituut CIVO-Toxicologie en Voeding TNO (author), Rutten, A.A.J.J.L. (author), Falke, H.E. (author), Catsburg, J.F. (author), Wortelboer, H.M. (author), Blaauboer, B.J. (author), Doorn, L. (author), van Leeuwen, F.X.R. (author), Theelen, R. (author), Rietjens, I.M.C.M. (author)
Assay conditions and results of cytochrome P-450 dependent 7-ethoxyresorufin (ER) and 7-pentoxyresorufin (PR) O-dealkylation (OD) by rat liver microsomes were compared by four laboratories in the Netherlands. Microsomal mixtures were prepared from control, 3-methylcholanthrene and phenobarbital pretreated animals, resulting in different levels...
article 1992
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Wortelboer, H.M. (author), van der Linden, E.C.M. (author), de Kruif, C.A. (author), Noordhoek, J. (author), Blaauboer, B.J. (author), van Bladeren, P.J. (author), Falke, H.E. (author)
Groups of male Wistar rats were fed semi-synthetic diets containing 0, 200 or 500 mg indole-3-carbinol (13C)/kg for 2, 7, 14 or 28 days. After 2 days, P-450 activities were already induced, but the isoenzyme pattern induced was different in the liver and the small intestine. Hepatic P4501A1, P4501A2 and P4502B1 apoprotein levels were dose...
article 1992
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Instituut voor Toxicologie en Voeding TNO (author), Blaauboer, B.J. (author), Mennes, W.C. (author), Wortelboer, H.M. (author)
bookPart 1993
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Instituut voor Toxicologie en Voeding TNO (author), van der Laan, J.W. (author), de Groot, G. (author), Wortelboer, H.M. (author), Noordhoek, J. (author)
Various strains of rats differ in their sensitivities towards benzodiazepines suggesting that variations in biological features are responsible for these differences. All reports concern inbred strains. We have studied male rats of three outbred Wistar strains with regard to the pharmacokinetics of desmethyldiazepam given as a single intravenous...
article 1993
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Instituut CIVO-Toxicologie en voeding TNO (author), Mennes, W.C. (author), Wortelboer, H.M. (author), Hassing, G.A. (author), van Sandwijk, K. (author), Timmerman, A. (author), Schmid, B.P. (author), Jahn, U. (author), Blaauboer, B.J. (author)
The hamster is known to display very high rates of monooxygenase-mediated biotransformation. In comparison with other species little knowledge has been gathered with respect to the nature of its cytochrome P450 enzymes and their respective inducibility. We studied the consequences of induction of P450 enzymes in rats and Syrian golden hamsters...
article 1993
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Instituut CIVO-Toxicologie en voeding TNO (author), Mennes, W.C. (author), Wortelboer, H.M. (author), Hassing, G.A. (author), van Sandwijk, K. (author), Timmerman, A. (author), Schmid, B.P. (author), Jahn, U. (author), Blaauboer, B.J. (author)
article 1994
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Cnubben, N.H.P. (author), Rietjens, I.M.C.M. (author), Wortelboer, H. (author), van Zanden, J.J. (author), van Bladeren, P.J. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
This review summarizes current knowledge on glutathione (GSH) associated cellular processes that play a central role in defense against oxidative stress. GSH itself is a critical factor in maintaining the cellular redox balance and has been demonstrated to be involved in regulation of cell signalling and repair pathways. Enhanced expression of...
article 2001
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Wortelboer, H.M. (author), Usta, M. (author), van der Velde, A.E. (author), Boersma, M.G. (author), Spenkelink, B. (author), van Zanden, J.J. (author), Rietjens, I.M.C.M. (author), van Bladeren, P.J. (author), Cnubben, N.H.P. (author)
The multidrug resistance proteins MRP1 and MRP2 are efflux transporters with broad substrate specificity, including glutathione, glucuronide, and sulfate conjugates. In the present study, the interaction of the dietary polyphenol curcumin with MRP1 and MRP2 and the interplay between curcumin-dependent MRP inhibition and its glutathione-dependent...
article 2003
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van Zanden, J.J. (author), Geraets, L. (author), Wortelboer, H.M. (author), van Bladeren, P.J. (author), Rietjens, I.M.C.M. (author), Cnubben, N.H.P. (author), TNO Voeding (author)
The objective of this study was to investigate the structural requirements necessary for inhibition of glutathione S-transferase P1-1 (GSTP1-1) and GS-X pump (MRP1 and MRP2) activity by structurally related flavonoids, in GSTP1-1 transfected MCF7 cells (pMTG5). The results reveal that GSTP1-1 activity in MCF7 pMTG5 cells can be inhibited by some...
article 2004
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Cnubben, N.H. (author), Wortelboer, H.M. (author), van Zanden, J.J. (author), Rietjens, I.M. (author), van Bladeren, P.J. (author), TNO Kwaliteit van Leven (author)
Membrane transport proteins belonging to the ATP-binding cassette (ABC) family of transport proteins play a central role in the defence of organisms against toxic compounds, including anticancer drugs. However, for compounds that are designed to display a toxic effect, this defence system diminishes their effectiveness. This is typically the...
article 2005
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van Zanden, J.J. (author), de Mul, A. (author), Wortelboer, H.M. (author), Usta, M. (author), van Bladeren, P.J. (author), Rietjens, I.M.C.M. (author), Cnubben, N.H.P. (author), TNO Kwaliteit van Leven (author)
In the present study, the effects of myricetin on either MRP1 or MRP2 mediated vincristine resistance in transfected MDCKII cells were examined. The results obtained show that myricetin can inhibit both MRP1 and MRP2 mediated vincristine efflux in a concentration dependent manner. The IC50 values for cellular vincristine transport inhibition by...
article 2005
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