document

Involvement of aspartic and glutamic residues in kringle-2 of tissue-type plasminogen activator in lysine binding, fibrin binding and stimulation of activity as revealed by chemical modification and oligonucleotide-directed mutagenesis

Weening-Verhoeff, E.J.D. (author), Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Rehberg, E.F. (author), Marotti, K.R. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)

article 1990

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Protein and messenger RNA levels of plasminogen activators and inhibitors analyzed in 22 human tumor cell lines

Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Verspaget, H.W. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)

In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase (u-PA) and tissue-type (u-PA) and their inhibitors PAI-1 and PAI-2 was studied. These four components may determine the net plasminogen activator activity, which is often associated with tumor development and metastatic processes. The amount of specific...

article 1990

document

Endotoxin induction of plasminogen activator and plasminogen activator inhibitor type 1 mRNA in rat tissues in vivo

Gaubius Instituut TNO (author), Quax, P.H.A. (author), van den Hoogen, M. (author), Verheijen, J.H. (author), Padro, T. (author), Zeheb, R. (author), Gelehrter, T.D. (author), van Berkel, T.J.C. (author), Kuiper, J. (author), Emeis, J.J. (author)

The tissue-specific distribution of tissue-type and urokinase-type plasminogen activator (t-PA and u-PA) and their inhibitor type 1 (PAI-1) was analyzed at mRNA level in five major rat organ tissues. t-PA mRNA was detected in lung, kidney, heart, and liver. u-PA mRNA was detected in kidney and lung. Presence of PA mRNA correlated with the...

article 1990

document

Plasminogen activator, extracellular proteolysis and cancer

Gezondheidsorganisatie TNO Gaubius Instituut TNO (author), Quax, P.H.A. (author)

doctoral thesis 1991

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Modulation of activities and RNA level of the components of the plasminogen activation system during fusion of human myogenic satellite cells in vitro

Quax, P.H.A. (author), Gaubius instituut (author)

other 1992

document

Eiwitafbraak en tumorcel migratie

Quax, P.H.A. (author), Gaubius instituut Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

article 1993

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Tissue-type plasminogen activator and its inhibitor in rat aorta: Effect of endotoxin

Padro, T. (author), Quax, P.H.A. (author), van den Hoogen, C.M. (author), Roholl, P. (author), Verheijen, J.H. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)

Plasminogen activator (PA) and PA inhibitor (PAI) antigen, activity, and mRNA were analyzed in the three layers of rat aorta, and the effect of endotoxin on PA and PAI was studied. All PA activity in aorta was identified as tissue-type PA (TPA) activity; no urokinase-type PA was detected. In the tunica adventitia TPA activity, TPA antigen, and...

article 1994

document

Tumor cells secrete an angiogenic factor that stimulates basic fibroblast growth factor and urokinase expression in vascular endothelial cells

Peverali, F.A. (author), Mandriota, S.J. (author), Ciana, P. (author), Marelli, R. (author), Quax, P. (author), Rifkin, D.B. (author), Della Valle, G. (author), Mignatti, P. (author), TNO Preventie en Gezondheid (author)

Culture medium conditioned by human SK-Hep1 hepatoma cells or mouse S180 sarcoma cells rapidly up-regulates endothelial cell expression of basic fibroblast growth factor (bFGF) and induces formation of capillary-like structures by vascular endothelial cells grown on three-dimensional fibrin gels (in vitro angiogenesis). Incubation of endothelial...

article 1994

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Role of plasminogen activators and their inhibitor PAI-1 in pericellular proteolysis of smooth muscle cells and endothelial cells

van Hinsbergh, V.W.M. (author), Quax, P.H.A. (author), Koolwijk, P. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)

Abstract

article 1994

document

Neue Gesichtspunkte der Melanompathogenese: Eigenschaften metastasierender und nicht-metastasierender humaner Melanomzellen

van Muijen, G.N.P. (author), de Vries, T.J. (author), Danen, E.H.J. (author), Quax, P. (author), Verheijen, J.H. (author), Ruiter, D.J. (author), TNO Preventie en Gezondheid (author)

In the pathogenesis qf human tumors the capacity of tumor cells to form metastases is one of the most crucial events. Ther^ore, the unravelling of molecular mechanisms involved in the process of metastasis is very important. Next to a great number of other factors, extracellular proteases and adhesion molecules of the integrin superfamily...

bookPart 1994

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Involvement of urokinase-type plasminogen activator in smooth muscle cell migration in vitro: Abstract

Quax, P.H.A. (author), TNO Preventie en Gezondheid (author)

article 1995

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Urokinase mediated, plasmin degradation of bone-like matrix

Ronday, H.K. (author), Smits, H.H. (author), Quax, P.H.A. (author), van der Pluijm, G. (author), Löwik, C.W.G.M. (author), Breedveld, F.C. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)

article 1995

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Regulation of plasminogen activation, matrix metalloproteinases and urokinase-type plasminogen activator-mediated extracellular matrix degradation in human osteosarcoma cell line MG63 by interleukin-1 alpha

de Bart, A.C.W. (author), Quax, P.H.A. (author), Löwik, C.W.G.M. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)

Plasmin-mediated extracellular proteolysis has been implicated in the degradation of bone in normal and pathological conditions. Normal and malignant osteoblasts can produce both tissue-type plasminogen activator (t- PA) and urokinase-type plasminogen activator (u-PA). We have used the osteosarcoma cell line MG63 to address the question of...

article 1995

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Mitogenic effects of urokinase plasminogen activator (u-PA) on melanoma cell lines (abstract)

Koopman, J.L. (author), Slomp, J. (author), de Bart, A.C.W. (author), Quax, P.H.A. (author), Verheijen, J.H. (author), TNO Preventie en gezondheid (author)

article 1996

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Different effects of lipopolysaccharide on plasminogen activator inhibitor-1 production in aortic media in vivo and in culture

van Leeuwen, R.T.J. (author), Quax, P.H.A. (author), Tippins, J.R. (author), Antoniw, J.W. (author), Andreotti, F. (author), Maseri, A. (author), Kluft, C. (author), Sperti, G. (author), Gaubius Instituut TNO (author)

Background: Lipopolysaccharide (endotoxin) has been shown to increase the expression of plasminogen activator inhibitor type-1 (PAI-1) in the vessel wall. Endotoxin is known to increase PAI-1 production in endothelial cells, but its action on smooth muscle cells (SMCs) is presently not clear. In this study we determined the effect of endotoxin...

article 1996

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Effects of infection with recombinant adenovirus on human vascular endothelial and smooth muscle cells

Quax, P.H.A. (author), Lamfers, M.L.M. (author), Grimbergen, J.M. (author), Teeling, J. (author), Hoeben, R.C. (author), van Nieuw Amerongen, G.P. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)

The plasminogen activation (PA) system is involved in vascular remodelling. Modulating its activity in vascular cells might be a way to interfere in processes such as angiogenesis and restenosis. Adenoviral vectors have become a favourable tool for direct gene transfer into vascular cells. In the interest of using adenoviral vectors to modulate...

article 1996

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Urokinase and tissue-type plasminogen activator stimulate human vascular smooth muscle cell migration

Wijnberg, M.J. (author), Nieuwenbroek, N.M.E. (author), Slomp, J. (author), Quax, P.H.A. (author), Verheijen, J.H. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)

The objective of this study was to investigate the role of the plasminogen activation system in the migration of human vascular smooth muscle cells in vitro. After wounding of confluent human smooth muscle cell cultures by stripping cells from their extracellular matrix, cells start to migrate from the wounded edge into the denuded area....

article 1996

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In vitro invasive behavior of melanoma cells transfected with urokinase plasminogen activator mutants

Slomp, J. (author), Koopman, J.L. (author), Quax, P.H.A. (author), de Bart, A.C.W. (author), Hennius, B.C. (author), Nieuwenbroek, N.M.E. (author), Verheijen, J.H. (author), TNO Preventie en gezondheid (author)

abstract

article 1996

document

The role of plasminogen activators in vascular pericellular proteolysis

Quax, P.H.A. (author), Koolwijk, P. (author), Verheijen, J.H. (author), van Hinsbergh, V.W.M. (author), TNO Preventie en gezondheid (author)

bookPart 1996

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The migration of human smooth muscle cells in vitro is mediated by plasminogen activation and can be inhibited by α2-macroglobulin receptor associated protein

Wijnberg, M.J. (author), Quax, P.H.A. (author), Nieuwenbroek, N.M.E. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)

The plasminogen activation system is thought to be important in cell migration processes. A role for this system during smooth muscle cell migration after vascular injury has been suggested from several animal studies. However, not much is known about its involvement in human vascular remodelling. We studied the involvement of the plasminogen...

article 1997