Print Email Facebook Twitter The small heat-shock protein αB-crystallin as candidate autoantigen in multiple sclerosis Title The small heat-shock protein αB-crystallin as candidate autoantigen in multiple sclerosis Author van Noort, J.M. van Sechel, A.C. Bajramovic, J.J. El Quagmiri, M. Polman, C.H. Lassmann, H. Ravid, R. TNO Preventie en Gezondheid Publication year 1995 Abstract The identification of key antigens in human autoimmune diseases is a crucial step towards the development of specific intervention. The autoantigen(s) relevant to multiple sclerosis (MS) probably reside in myelin of the central nervous system, the target of the disease. Here we examine proliferative responses of human peripheral blood T cells to the complete collection of myelin proteins fractionated by reversed-phase high-performance liquid chromatography. Myelin isolated from MS-affected brain contained a single protein fraction to which T cells from MS patients and from healthy controls showed dominant responses. This highly immunogenic protein was identified as αB-crystallin, a small heat-shock protein. Immunohistochemical examination of MS lesions revealed the presence of oligodendrocytes and astrocytes with raised αB-crystallin expression, which were not found in unaffected myelin. Our findings indicate that αB-crystallin serves as immunodominant myelin antigen to human T cells when expressed at the elevated levels found in active MS lesions. Chemicals/CAS: crystallin, 11046-99-4; Autoantigens; Crystallins; Heat-Shock Proteins; Myelin Proteins Subject BiologyAstrocytesAutoantigensCells, CulturedCrystallinsHeat-Shock ProteinsHumanLymphocyte ActivationMultiple SclerosisMyelin ProteinsMyelin SheathOligodendrogliaT-Lymphocytes To reference this document use: http://resolver.tudelft.nl/uuid:fd4b5f3b-dd3e-45b2-b59e-be04994d8752 TNO identifier 232924 ISSN 0028-0836 Source Nature, 375 (6534), 798-801 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.