Title
Evaluation of the antibacterial spectrum of drosocin analogues
Author
Bikker, F.J.
Kaman-van Zanten, W.E.
de Vries-van de Ruit, A.M.B.C.
Voskamp-Visser, I.
van Hooft, P.A.V.
Mars-Groenendijk, R.H.
de Visser, P.C.
Noort, D.
TNO Defensie en Veiligheid
Publication year
2006
Abstract
Drosocin is a 19-mer, cationic antimicrobial peptide from Drosophila melanogaster. The aim of the study was to examine the antibacterial spectrum of unglycosylated drosocin analogues. Furthermore, the amino acid sequence of DnaK, drosocin's intracellular target, from susceptible species was aligned and studied for sequence homology. From this a panel of 31 bacterial strains, including Salmonella strains with truncated lipopolysaccharide structures, was tested for susceptibility towards three drosocin analogues. Available bacterial DnaK amino acid sequences were retrieved from the ExPASy proteomics server of the Swiss Institute of Bioinformatics studied for sequence homology. Seventeen of the 31 strains tested were susceptible for the drosocin analogues. Minimal inhibitory concentration values against mainly Gram-negative bacteria ranged from 3.1 to 100 μm. With the exception of Micrococcus luteus and Xanthomonas campestris all drosocin analogue-susceptible strains were Enterobacteriaceae showing a high DnaK amino acid sequence homology. © 2006 The Authors. Chemicals / CAS: drosocin, 179048-25-0; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; drosocin, 149924-99-2; Drosophila Proteins; Glycopeptides
Subject
Antimicrobial peptide
Biological agents
DnaK
Drosocin
Lipopolysaccharide
Spectrum
Dosocin
Lipopolysaccharide
Protein DnaK
Amino acid sequence
Antibacterial activity
Antibiotic sensitivity
Article
Bacterial strain
Controlled study
Enterobacteriaceae
Gram negative bacterium
Micrococcus luteus
Minimum inhibitory concentration
Nonhuman
Priority journal
Salmonella
Sequence homology
Xanthomonas campestris
Amino Acid Sequence
Anti-Bacterial Agents
Antimicrobial Cationic Peptides
Drosophila Proteins
Glycopeptides
Gram-Negative Bacteria
Gram-Positive Bacteria
Microbial Sensitivity Tests
Bacteria (microorganisms)
Drosophila melanogaster
Enterobacteriaceae
Micrococcus luteus
Negibacteria
Salmonella
Xanthomonas campestris
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http://resolver.tudelft.nl/uuid:fbc911d7-482a-46f6-8c24-bd0a44a28dae
DOI
https://doi.org/10.1111/j.1747-0285.2006.00424.x
TNO identifier
239455
ISSN
1747-0277
Source
Chemical Biology and Drug Design, 68 (3), 148-153
Document type
article