Title
New monoclonal antibodies against the putative immunosuppressive site of retroviral p15E
Author
Lang, M.S.
Oostendorp, R.A.J.
Simons, P.J.
Boersma, W.
Knegt, P.
van Ewijk, W.
Publication year
1994
Abstract
Both retroviral infections as well as human tumors may cause immunosuppression. One of the factors involved in immunosuppression in patients with squamous cell carcinoma of the head and neck (SCC-HN) is a protein related in the retroviral protein p15E. A conserved, 17-amino acid sequence represents the immunosuppressive epitope of retroviral p15E. In order to study the relationship between SCC-HN associated immunosuppression and retroviral p15E, we produced three new monoclonal antibodies (MAbs: ER- IS1, ER-IS2, and ER-185) directed against the immunosuppressive synthetic CKS-17 peptide. These MAbs react with the immunosuppressive peptide (in enzyme-linked immunosorbent assay), with human tumor cell lines (in FACScan analysis), with retroviral p15E (on Western blot), and with cryostat sections of SCC-HN tumor tissue. In addition, the MAbs neutralize the immunosuppressive low molecular weight factors present in sera of patients with SCC-HN. These results show that retroviral p15E and the immunosuppressive factors associated with SCC-HN share a conserved immunosuppressive epitope and that MAbs against this epitope can be used for detection and centralization of the tumor-associated immunosuppressive protein(s).
Subject
Monoclonal antibody
Monoclonal antibody er is1
Monoclonal antibody er is2
Monoclonal antibody er is5
Unclassified drug
Airus protein
Antibody production
Antigen antibody reaction
Antigen recognition
Cancer cell culture
Cancer immunotherapy
Head and neck cancer
Human
Human cell
Retrovirus
Squamous cell carcinoma
Virus infection
Amino Acid Sequence
Antibodies, Monoclonal
Binding Sites, Antibody
Blotting, Western
Carcinoma, Squamous Cell
Cell Line
Conserved Sequence
Enzyme-Linked Immunosorbent Assay
Epitopes
Head and Neck Neoplasms
Immunoblotting
Immunohistochemistry
Immunosuppression
Laryngeal Neoplasms
Lung Neoplasms
Lymphoma, Large-Cell
Molecular Sequence Data
Peptides
Rauscher Virus
Retroviridae
Retroviridae Proteins, Oncogenic
Tumor Cells, Cultured
Antibodies, Monoclonal
Binding Sites, Antibody
Epitopes
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TNO identifier
280590
ISSN
0008-5472
Source
Cancer Research, 54 (7), 1831-1836
Document type
article