Print Email Facebook Twitter Retrospective detection of sulfur mustard exposure by mass spectrometric analysis of adducts to albumin and hemoglobin: An in vivo study Title Retrospective detection of sulfur mustard exposure by mass spectrometric analysis of adducts to albumin and hemoglobin: An in vivo study Author Noort, D. Fidder, A. Degenhardt-Langelaan, C.E.A.M. Hulst, A.G. TNO Defensie en Veiligheid Publication year 2008 Abstract The persistence in rats of sulfur mustard adducts to albumin and hemoglobin was studied in vivo after exposure (intravenously; 0.3 mg/kg; approximately 0.1 LD50) of rats to sulfur mustard. The albumin adduct (S-HETE)Cys-Pro-Tyr was detectable up to 7 days after the exposure, while the adduct to the N-terminal valine in hemoglobin was still detected after 28 days. The decrease in adduct levels corresponded well with the half-life time of albumin in rats and with the lifetime of the rat erythrocyte. Remarkably, the N-terminal valine adduct to hemoglobin increased during the first three days, which implies that there is still free sulfur mustard present during that time. In contrast, the corresponding albumin adduct levels did not increase during this time period. The free sulfur mustard might have accumulated in the erythrocyte cell membrane. Subject AlbuminAlkylationAnimal experimentArticleBlood analysisCell membraneChemical reactionControlled studyEnvironmental exposureErythrocyteIn vivo studyNonhumanAlkylationAnimalsAffinity chromatographyLiquid chromatographyEnvironmental exposureEnvironmental monitoringInjections, IntravenousMalePronaseRatsWistarMass spectrometryElectrospray ionizationTandem mass spectrometryRattusHemoglobin 9008-02-0Mustard gas 505-60-2Biological markersHemoglobinsPronase, EC 3.4.24.-Serum albumin To reference this document use: http://resolver.tudelft.nl/uuid:ecbdecf0-0568-4c9a-b44d-da369fc7f327 TNO identifier 240608 ISSN 0146-4760 Source Journal of Analytical Toxicology, 32 (1), 25-30 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.