Title
Retrospective detection of sulfur mustard exposure by mass spectrometric analysis of adducts to albumin and hemoglobin: An in vivo study
Author
Noort, D.
Fidder, A.
Degenhardt-Langelaan, C.E.A.M.
Hulst, A.G.
TNO Defensie en Veiligheid
Publication year
2008
Abstract
The persistence in rats of sulfur mustard adducts to albumin and hemoglobin was studied in vivo after exposure (intravenously; 0.3 mg/kg; approximately 0.1 LD50) of rats to sulfur mustard. The albumin adduct (S-HETE)Cys-Pro-Tyr was detectable up to 7 days after the exposure, while the adduct to the N-terminal valine in hemoglobin was still detected after 28 days. The decrease in adduct levels corresponded well with the half-life time of albumin in rats and with the lifetime of the rat erythrocyte. Remarkably, the N-terminal valine adduct to hemoglobin increased during the first three days, which implies that there is still free sulfur mustard present during that time. In contrast, the corresponding albumin adduct levels did not increase during this time period. The free sulfur mustard might have accumulated in the erythrocyte cell membrane.
Subject
Albumin
Alkylation
Animal experiment
Article
Blood analysis
Cell membrane
Chemical reaction
Controlled study
Environmental exposure
Erythrocyte
In vivo study
Nonhuman
Alkylation
Animals
Affinity chromatography
Liquid chromatography
Environmental exposure
Environmental monitoring
Injections, Intravenous
Male
Pronase
Rats
Wistar
Mass spectrometry
Electrospray ionization
Tandem mass spectrometry
Rattus
Hemoglobin 9008-02-0
Mustard gas 505-60-2
Biological markers
Hemoglobins
Pronase, EC 3.4.24.-
Serum albumin
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TNO identifier
240608
ISSN
0146-4760
Source
Journal of Analytical Toxicology, 32 (1), 25-30
Document type
article