Print Email Facebook Twitter Expression of heterologous genes in Schizophyllum commune is often hampered by the formation of truncated transcripts Title Expression of heterologous genes in Schizophyllum commune is often hampered by the formation of truncated transcripts Author Schuren, F.H.J. Wessels, J.G.H. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1998 Abstract GPD regulatory sequences were used to express a phleomycin resistance gene (Sh ble) in Schizophyllum commune, resulting in high numbers of phleomycin-resistant transformants. Attempts to express heterologous genes coding for hygromycin B phosphotransferase (hph), aminoglycoside phosphotransferase (apt), β-glucuronidase (uidA) and β-galactosidase (lacZ) using the same regulatory sequences were not successful and no mRNA could be detected. Cloning the hph and uidA genes in an internally deleted GPD gene resulted in truncated transcripts which ended within the 5'-parts of the heterologous genes. Cloning of the same genes as transcriptional fusions downstream from the Sh ble gene also resulted in truncated transcripts ending in the 5'-parts of these heterologous genes. It is suggested that AT-rich sequences in heterologous genes might be involved in generating these truncated transcripts, thereby preventing expression in S. commune. Subject NutritionBasidiomycete fungusHeterologous gene expressionSchizophyllum communeTranscription terminationAminoglycosideBeta glucuronidaseHygromycin bMessenger rnaPhleomycinPhosphotransferaseAntibiotic resistanceFungal geneticsMolecular cloningNonhumanPriority journalSchizophyllum communeBase Sequencebeta-GalactosidaseDNA, FungalGene Expression Regulation, EnzymologicGene Expression Regulation, FungalGlucuronidaseGlyceraldehyde-3-Phosphate DehydrogenasesKanamycin KinaseMolecular Sequence DataPhosphotransferases (Alcohol Group Acceptor)SchizophyllumFungiSchizophyllum commune To reference this document use: http://resolver.tudelft.nl/uuid:ec5dcd7b-b691-4ded-9a2d-8ccaf325ba57 DOI https://doi.org/10.1007/s002940050321 TNO identifier 234363 ISSN 0172-8083 Source Current Genetics, 33 (2), 151-156 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.