Title
Expression of heterologous genes in Schizophyllum commune is often hampered by the formation of truncated transcripts
Author
Schuren, F.H.J.
Wessels, J.G.H.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1998
Abstract
GPD regulatory sequences were used to express a phleomycin resistance gene (Sh ble) in Schizophyllum commune, resulting in high numbers of phleomycin-resistant transformants. Attempts to express heterologous genes coding for hygromycin B phosphotransferase (hph), aminoglycoside phosphotransferase (apt), β-glucuronidase (uidA) and β-galactosidase (lacZ) using the same regulatory sequences were not successful and no mRNA could be detected. Cloning the hph and uidA genes in an internally deleted GPD gene resulted in truncated transcripts which ended within the 5'-parts of the heterologous genes. Cloning of the same genes as transcriptional fusions downstream from the Sh ble gene also resulted in truncated transcripts ending in the 5'-parts of these heterologous genes. It is suggested that AT-rich sequences in heterologous genes might be involved in generating these truncated transcripts, thereby preventing expression in S. commune.
Subject
Nutrition
Basidiomycete fungus
Heterologous gene expression
Schizophyllum commune
Transcription termination
Aminoglycoside
Beta glucuronidase
Hygromycin b
Messenger rna
Phleomycin
Phosphotransferase
Antibiotic resistance
Fungal genetics
Molecular cloning
Nonhuman
Priority journal
Schizophyllum commune
Base Sequence
beta-Galactosidase
DNA, Fungal
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Glucuronidase
Glyceraldehyde-3-Phosphate Dehydrogenases
Kanamycin Kinase
Molecular Sequence Data
Phosphotransferases (Alcohol Group Acceptor)
Schizophyllum
Fungi
Schizophyllum commune
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http://resolver.tudelft.nl/uuid:ec5dcd7b-b691-4ded-9a2d-8ccaf325ba57
DOI
https://doi.org/10.1007/s002940050321
TNO identifier
234363
ISSN
0172-8083
Source
Current Genetics, 33 (2), 151-156
Document type
article