Title
Influence of inflammatory cells and serum on the performance of implantable glucose sensors
Author
Gerritsen, M.
Jansen, J.A.
Kros, A.
Vriezema, D.M.
Sommerdijk, N.A.J.M.
Nolte, R.J.M.
Lutterman, J.A.
van Hövell, S.W.F.M.
van der Gaag, A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2001
Abstract
The objective of this investigation was to evaluate the influence of polymorphonuclear granulocytes on the performance of uncoated and cellulose acetate/Nafion® coated amperometric glucose sensors in vitro. The response of these sensors was also investigated in serum. Uncoated and coated sensors showed lower sensitivities to glucose, with a significant drift in sensor output upon exposure to serum or leukocytes. Although the use of a coating resulted in higher sensitivity, the progressive loss of output was not completely prevented. Stimulated granulocytes were shown to excrete components, probably catalase and myeloperoxidase, which consumed the hydrogen peroxide formed by the oxidation of glucose. In addition, adsorbed serum proteins formed a diffusional barrier for glucose. Furthermore, serum was found to contain low-molecular weight components that alone inhibited glucose oxidase activity. Based on preliminary electrochemical results, we postulate that rabbit serum contains oxidizing substrates that compete with molecular oxygen for the acceptance of electrons from the oxidized enzyme. Consequently, future efforts should be aimed at elucidating the mechanisms involved in the interference of unknown serum components with electron transfer. In addition, further investigations have to be performed to develop an outer membrane that minimizes protein adsorption as well as the actions of inflammatory cells. (C) 2000 John Wiley and Sons, Inc. Chemicals/CAS: Biocompatible Materials; Blood Glucose; Buffers; Cellulose, 9004-34-6; Coated Materials, Biocompatible; Enzymes, Immobilized; Fluorocarbon Polymers; Glucose Oxidase, EC 1.1.3.4; perfluorosulfonic acid, 39464-59-0; Zymosan, 9010-72-4
Subject
Nutrition
Animals
Biocompatible Materials
Biosensing Techniques
Blood Glucose
Buffers
Cellulose
Coated Materials, Biocompatible
Enzymes, Immobilized
Fluorocarbon Polymers
Glucose Oxidase
Granulocytes
Inflammation
Neutrophils
Prostheses and Implants
Rabbits
Zymosan
To reference this document use:
http://resolver.tudelft.nl/uuid:eb979275-c1c8-4cee-87b8-f121244e8550
DOI
https://doi.org/10.1002/1097-4636(200101)54:1<69::aid-jbm8>3.0.co;2-q
TNO identifier
56902
ISSN
0021-9304
Source
Journal of Biomedical Materials Research, 54 (1), 69-75
Document type
article