Print Email Facebook Twitter Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced Title Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced Author Hijum, S.A.F.T. Bonting, K. van der Maarel, M.J.E.V. Dijkhuizen, L. TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO Publication year 2001 Abstract Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells. © 2001 Pubblished by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies. Chemicals/CAS: Fructans; Hexosyltransferases, EC 2.4.1.-; levan fructotransferase, EC 2.4.1.-; levan, 9013-95-0; levansucrase, EC 18.104.22.168; Polysaccharides, Bacterial; Sucrose, 57-50-1 Subject BiologyAmino Acid SequenceFructansHexosyltransferasesLactobacillusMagnetic Resonance SpectroscopyMolecular Sequence DataMolecular WeightPolysaccharides, BacterialSequence AlignmentSucroseLactobacillusLactobacillus reuteriLactobacillus sp.Posibacteria To reference this document use: http://resolver.tudelft.nl/uuid:e78a4fe1-aed4-4e93-8aa6-137915e8d484 TNO identifier 72488 Source FEMS Microbiology Letters, 205, 323-328 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.