Title
Frequency of HPRT mutants in humans exposed to vinyl chloride via an environmental accident
Author
TNO Kwaliteit van Leven TNO BIBRA
Becker, R.
Nikolova, T.
Wolff, I.
Lovell, D.
Hüttner, E.
Foth, H.
Publication year
2001
Abstract
The mutant frequency (MF) in the hypoxanthine-guanine-phosphoribosyl-transferase (HPRT) locus of peripheral blood T-lymphocytes was measured in a population environmentally exposed to vinyl chloride - a toxic and carcinogenic substance through an accidental release into the atmosphere. It was compared to MF in a control group of unexposed individuals. Both groups were re-investigated in a follow-up study, 2 years later. No significant difference could be observed in MF between exposed and controls either at the accident nor in the follow-up study. Approximately the same mean HPRT mutant frequencies were observed for both groups in T-lymphocytes from blood samples obtained shortly after the accident and from the follow-up blood samples. Both groups showed a higher mean MF in the re-investigation samples which is most probably due to the significantly lower average cloning efficiency (CE) under non-selective conditions and because of the inverse relationship between CE and MF. The exposed population showed a higher mean T-cell CE at the initial blood sampling as compared to the control group. The concurrent cytogenetic analyses of peripheral lymphocytes showed a significant increase in cells with aberrations in the exposed population. Clastogenic but not mutagenic activity of vinyl chloride was observed in our study. © 2001 Elsevier Science B.V. Chemicals/CAS: Air Pollutants; Hypoxanthine Phosphoribosyltransferase, EC 2.4.2.8; Mutagens; Vinyl Chloride, 75-01-4
Subject
Accidental environmental exposure
HPRT-gene
Human T-lymphocytes
In vivo
Mutant frequency
Vinyl chloride
hypoxanthine phosphoribosyltransferase
vinyl chloride
accident
adult
article
blood sampling
controlled study
environmental exposure
female
follow up
genotoxicity
human
human cell
human experiment
male
mutation rate
normal human
priority journal
T lymphocyte
Adult
Air Pollutants
Clone Cells
Environmental Exposure
Female
Follow-Up Studies
Humans
Hypoxanthine Phosphoribosyltransferase
Male
Middle Aged
Mutagenesis
Mutagens
Mutation
T-Lymphocytes
Vinyl Chloride
To reference this document use:
http://resolver.tudelft.nl/uuid:e35c966c-5b6d-49bd-8017-d566a5a08e28
DOI
https://doi.org/10.1016/s1383-5718(01)00182-6
TNO identifier
280354
ISSN
1383-5718
Source
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 494 (494), 87-96
Document type
article