Print Email Facebook Twitter Air-liquid interface In Vitro models for respiratory toxicology research: Consensus workshop and recommendations Title Air-liquid interface In Vitro models for respiratory toxicology research: Consensus workshop and recommendations Author LaCroix, G. Koch, W. Ritter, D. Gutleb, A.C. Larsen, S.T. Loret, T. Zanetti, F. Constant, S. Chortarea, S. Rothen-Rutishauser, B. Hiemstra, P.S. Frejafan, E. Hubert, P. Gribaldo, L. Kearns, P. Aublant, J.M. Diabaté, S. Weiss, C. de Groot, A. Kooter, I.M. Publication year 2018 Abstract In vitro air–liquid interface (ALI) cell culture models can potentially be used to assess inhalation toxicology endpoints and are usually considered, in terms of relevancy, between classic (i.e., submerged) in vitro models and animal-based models. In some situations that need to be clearly defined, ALI methods may represent a complement or an alternative option to in vivo experimentations or classic in vitro methods. However, it is clear that many different approaches exist and that only very limited validation studies have been carried out to date. This means comparison of data from different methods is difficult and available methods are currently not suitable for use in regulatory assessments. This is despite inhalation toxicology being a priority area for many governmental organizations. In this setting, a 1-day workshop on ALI in vitro models for respiratory toxicology research was organized in Paris in March 2016 to assess the situation and to discuss what might be possible in terms of validation studies. The workshop was attended by major parties in Europe and brought together more than 60 representatives from various academic, commercial, and regulatory organizations. Following plenary, oral, and poster presentations, an expert panel was convened to lead a discussion on possible approaches to validation studies for ALI inhalation models. A series of recommendations were made and the outcomes of the workshop are reported. Subject Urban Mobility & EnvironmentEMS - Environmental Modelling, Sensing & AnalysisELSS - Earth, Life and Social SciencesEnvironment & SustainabilityEnvironmentUrbanisationAir-liiquid interfaceIn-vitroInhalationLung cell modelsToxicologyValidation To reference this document use: http://resolver.tudelft.nl/uuid:dde8f8ae-5b2f-4a2f-8121-396076cfa6dd TNO identifier 787998 Source Applied in Vitro Toxicology, 4 (2), 1-16 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.