Title
D-Tagatose increases butyrate production by the colonic microbiota in healthy men and women
Author
Venema, K.
Vermunt, S.H.F.
Brink, E.J.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
D-Tagatose is partly absorbed in the stomach and small intestine. Most of it is fermented by the large intestinal microbiota. The effect of D-tagatose on the composition of the microbiota and production of short chain fatty acids (SCFAs) was studied in vivo and in vitro. Gastrointestinal (GI) complaints were also studied. The in vivo study was performed according to a randomized, placebo-controlled, double-blind, five-way cross-over design in healthy subjects (12 men and 18 women). All subjects consumed 30 g raspberry jam containing 7.5 or 12.5 g D-tagatose, 7.8 g fructo-oligosaccharides (positive reference), 7.6 g D-tagatose plus 7.5 g fructo-oligosaccharides, or 15.1 g sucrose (negative reference) at breakfast for 2 weeks in different orders. At the end of each treatment period lipids and safety parameters in blood and GI complaints were evaluated by questionnaires, and faecal microbiota and SCFAs were measured. Furthermore, test-tube incubations of faecal slurries with D-tagatose, fructo-oligosaccharides and sucrose were performed. An in vitro model simulating the large intestine was used to assess the mechanistic effect of D-tagatose on microbiota composition and SCFA production. The high-tagatose treatment resulted in increased numbers of faecal lactobacilli in men, but not in women. Also in vitro, lactobacilli increased. Both the test-tube incubations of fresh faeces from the in vivo study with D-tagatose and the study in the in vitro model showed increased butyrate production after all treatments with D-tagatose. High-tagatose, but not low-tagatose, resulted in a slightly increased defecation frequency and stools of thinner consistency. Only a few GI complaints were reported. The data indicate that daily consumption of 7.5 or 12.5 g D-tagatose may lead to increased production of butyrate and to an increase of lactobacilli, without serious GI complaints. In view of the health-promoting effects of butyrate and lactobacilli, D-tagatose may be considered a prebiotic substrate. © 2005 Taylor & Francis Group Ltd.
Subject
Health
Biomedical Research
Butyrate
Colon
D-tagatose
Gastrointestinal health
Healthy subjects
Microbiota
butyric acid
fructose oligosaccharide
lipid
placebo
prebiotic agent
short chain fatty acid
sucrose
tagatose
article
clinical trial
colon flora
controlled clinical trial
controlled study
crossover procedure
defecation
double blind procedure
female
gastrointestinal symptom
human
human experiment
in vitro study
in vivo study
incubation time
intestine absorption
Lactobacillus
male
normal human
parameter
priority journal
questionnaire
randomized controlled trial
safety
stomach absorption
Acacia acuminata
Microbiota
Rubus glaucus
To reference this document use:
http://resolver.tudelft.nl/uuid:db5b381f-3b71-4e4c-a476-10f70c027c64
DOI
https://doi.org/10.1080/08910600510035093
TNO identifier
238422
ISSN
0891-060X
Source
Microbial Ecology in Health and Disease, 17 (1), 47-57
Document type
article