Print Email Facebook Twitter Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies Title Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies Author Lozano-Ojalvo, D. Benede, S. Antunes, C.M. Bavaro, S.L. Bouchaud, G. Costa, A. Denery-Papini, S. Diaz-Perales, A. Garrido-Arandia, M. Gavrovic-Jankulovic, M. Hayen, S. Martinez-Blanco, M. Molina, E. Monaci, L. Pieters, R.H.H. Villemin, C. Wichers, H.J. Wroblewska, B. Willemsen, L.E.M. Roggen, E.L. van Bilsen, J.H.M. Publication year 2019 Abstract Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches. A© 2019 Elsevier Ltd Subject LifeRAPID - Risk Analysis for Products in DevelopmentNutritionAdverse outcome pathwayDendritic cellsEpithelial cellsIgE-mediated food allergyIn vitro modelsT and B cellsAllergiesCytologyImmune systemProteinsRisk assessmentAdverse outcomesB cellsDendritic cellsEpithelial cellsFood allergiesIn-vitro modelsCells To reference this document use: http://resolver.tudelft.nl/uuid:d8057761-d81a-470b-8d88-0490a66e2a65 DOI https://doi.org/10.1016/j.tifs.2019.01.014/ TNO identifier 862374 ISSN 0924-2244 Source Trends in Food Science and Technology, 85, 307-319 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.