Print Email Facebook Twitter A mixed-species microarray for identification of food spoilage bacilli Title A mixed-species microarray for identification of food spoilage bacilli Author Caspers, M.P.M. Schuren, F.H.J. van Zuijlen, A.C.M. Brul, S. Montijn, R.C. Abee, T. Kort, R. Publication year 2011 Abstract Failure of food preservation is frequently caused by thermostable spores of members of the Bacillaceae family, which show a wide spectrum of resistance to cleaning and preservation treatments. We constructed and validated a mixed-species genotyping array for 6 Bacillus species, including Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus sporothermodurans, Bacillus cereus and Bacillus coagulans, and 4 Geobacillus species, including Geobacillus stearothermophilus, Geobacillus thermocatenulatus, Geobacillus toebii and Geobacillus sp., in order to track food spoilage isolates from ingredient to product. The discriminating power of the array was evaluated with sets of 42 reference and 20 test strains. Bacterial isolates contain a within-species-conserved core genome comprising 68-88% of the entire genome and a non-conserved accessory genome comprising 7-22%. The majority of the core genome markers do not hybridise between species, thus they allow for efficient discrimination at the species level. The accessory genome array markers provide high-resolution discrimination at the level of individual isolates from a single species. In conclusion, the reported mixed-species microarray contains discriminating markers that allow rapid and cost-effective typing of Bacillus food spoilage bacteria in a wide variety of food products. © 2010 Elsevier Ltd. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesNutritionBacillusBacterial sporesComparative genome hybridisationFood spoilageGenotypingGeobacillusMicroarrayTracking and tracing To reference this document use: http://resolver.tudelft.nl/uuid:d2f57cd4-e6f9-42e8-b726-9e4cf41b1956 DOI https://doi.org/10.1016/j.fm.2010.03.010 TNO identifier 427543 ISSN 0740-0020 Source Food Microbiology, 28 (2), 245-251 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.