Title
Lipolysis of very low density lipoproteins by heparan sulfate proteoglycan-bound lipoprotein lipase
Author
Gaubius Instituut TNO
de Man, F.H.A.F.
de Beer, F.
van der Laarse, A.
Smelt, A.H.M.
Havekes, L.M.
Publication year
1997
Abstract
An in vitro assay to study lipolysis of very low density lipoproteins (VLDL) by heparan sulfate proteoglycan (HSPG-bound lipoprotein lipase (LPL) was developed. Optimal conditions for VLDL lipolysis by HSPG-bound LPL were obtained by incubating plastic wells with 0.5 ug HSPG and 1.5 ug LPL, subsequently. Control experiments with heparinase indicate that at least 90% of the LPL activity is derived from LPL bound to heparan sulfate chains. For HSPG-LPL-mediated lipolysis, the apparent K(m) and V(max) values were 0.36 ± 0.11 mM VLDL-triglycerides and 1.2 ± 0.1 uM free fatty acids/min·ng LPL, respectively. The mean intra-assay and inter-assay coefficients of variance were 5% and 8%, respectively.
Subject
Animals
Binding, Competitive
Cattle
Fatty Acids
Heparan Sulfate Proteoglycans
Heparin Lyase
Humans
Iodine Radioisotopes
Kinetics
Lipolysis
Lipoprotein Lipase
Lipoproteins, VLDL
Protein Binding
Radioligand Assay
Triglycerides
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TNO identifier
234255
ISSN
0022-2275
Source
Journal of Lipid Research, 38 (38), 2465-2472
Document type
article