Print Email Facebook Twitter Proteolytic shedding of the macrophage scavenger receptor CD163 in multiple sclerosis Title Proteolytic shedding of the macrophage scavenger receptor CD163 in multiple sclerosis Author Fabriek, B.O. Møller, H.J. Vloet, R.P.M. van Winsen, L.M. Hanemaaijer, R. Teunissen, C.E. Uitdehaag, B.M.J. van den Berg, T.K. Dijkstra, C.D. TNO Kwaliteit van Leven Publication year 2007 Abstract The scavenger receptor CD163 is selectively expressed on tissue macrophages and human monocytes. CD163 has been implicated to play a role in the clearance of hemoglobin and in the regulation of cytokine production by macrophages. Membrane CD163 can be cleaved by matrix metalloproteinases (MMP) resulting in soluble CD163 (sCD163). In the present report the shedding of CD163 was investigated in multiple sclerosis (MS). An upregulation of plasma sCD163 and a down regulation of membrane CD163 in MS patients compared to healthy controls was observed. The levels of plasma sCD163 correlated with plasma MMP-9 levels in controls, but not in MS patients. Moreover, evidence was obtained for CD163-cleaving MMP activity in plasma of MS patients. Finally, the increased proteolytic shedding of CD163 correlated to reduced plasma levels of circulating inflammatory cytokines. Collectively, our results provide evidence for proteolytic shedding of CD163 in MS and suggest a possible link to cytokine production. © 2007 Elsevier B.V. All rights reserved. Subject HealthBiomedical ResearchCD163MetalloproteinaseMonocytesMultiple sclerosisScavenger receptorsCD163CD163 antigencytokinegelatinase Bscavenger receptoradultagedanalytic methodarticlecomparative studycontrolled studycytokine productiondown regulationenzyme activityfemalehumanmajor clinical studymalemultiple sclerosispriority journalprotein degradationupregulationAdolescentAdultAgedAged, 80 and overAnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticCell Line, TransformedCricetinaeCricetulusCytokinesEnzyme InhibitorsEnzyme-Linked Immunosorbent AssayFemaleHumansHydrocortisoneMaleMatrix Metalloproteinase 2Matrix Metalloproteinase 9Middle AgedMultiple SclerosisReceptors, Cell SurfaceStatistics, NonparametricTransfection To reference this document use: http://resolver.tudelft.nl/uuid:cb9f954a-c3cf-4672-9e82-774fe6fd067e DOI https://doi.org/10.1016/j.jneuroim.2007.04.016 TNO identifier 240067 ISSN 0165-5728 Source Journal of Neuroimmunology, 187 (1-2), 179-186 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.