Title
Proteolytic shedding of the macrophage scavenger receptor CD163 in multiple sclerosis
Author
Fabriek, B.O.
Møller, H.J.
Vloet, R.P.M.
van Winsen, L.M.
Hanemaaijer, R.
Teunissen, C.E.
Uitdehaag, B.M.J.
van den Berg, T.K.
Dijkstra, C.D.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
The scavenger receptor CD163 is selectively expressed on tissue macrophages and human monocytes. CD163 has been implicated to play a role in the clearance of hemoglobin and in the regulation of cytokine production by macrophages. Membrane CD163 can be cleaved by matrix metalloproteinases (MMP) resulting in soluble CD163 (sCD163). In the present report the shedding of CD163 was investigated in multiple sclerosis (MS). An upregulation of plasma sCD163 and a down regulation of membrane CD163 in MS patients compared to healthy controls was observed. The levels of plasma sCD163 correlated with plasma MMP-9 levels in controls, but not in MS patients. Moreover, evidence was obtained for CD163-cleaving MMP activity in plasma of MS patients. Finally, the increased proteolytic shedding of CD163 correlated to reduced plasma levels of circulating inflammatory cytokines. Collectively, our results provide evidence for proteolytic shedding of CD163 in MS and suggest a possible link to cytokine production. © 2007 Elsevier B.V. All rights reserved.
Subject
Health
Biomedical Research
CD163
Metalloproteinase
Monocytes
Multiple sclerosis
Scavenger receptor
sCD163
CD163 antigen
cytokine
gelatinase B
scavenger receptor
adult
aged
analytic method
article
comparative study
controlled study
cytokine production
down regulation
enzyme activity
female
human
major clinical study
male
multiple sclerosis
priority journal
protein degradation
upregulation
Adolescent
Adult
Aged
Aged, 80 and over
Animals
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Cell Line, Transformed
Cricetinae
Cricetulus
Cytokines
Enzyme Inhibitors
Enzyme-Linked Immunosorbent Assay
Female
Humans
Hydrocortisone
Male
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Middle Aged
Multiple Sclerosis
Receptors, Cell Surface
Statistics, Nonparametric
Transfection
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DOI
https://doi.org/10.1016/j.jneuroim.2007.04.016
TNO identifier
240067
ISSN
0165-5728
Source
Journal of Neuroimmunology, 187 (1-2), 179-186
Document type
article