Title
A pilot study to investigate effects of inulin on Caco-2 cells through in vitro metabolic fingerprinting
Author
Lamers, R.-J.A.N.
Wessels, E.C.H.H.
van de Sandt, J.J.M.
Venema, K.
Schaafsma, G.
van der Greef, J.
van Nesselrooij, J.H.J.
Publication year
2003
Abstract
Metabolic fingerprints are novel measurement tools to evaluate the biochemical status of a living organism by using 1H NMR and multivariate data analysis (MVDA). In this way, a quick evaluation of changes in health or diseased state can be made, reflected in alterations of metabolic patterns. Normally, metabolic finger-printing is based on in vivo studies. These studies often represent a labor-intensive and expensive manner of investigation. In vitro studies are not hampered by these disadvantages, thus constituting an interesting alternative. In this research, results are presented of a pilot experiment in which metabolic fingerprinting was combined with an in vitro model. For this purpose, differentiated Caco-2 cells were exposed to inulin and its fermentative metabolites, both dissolved in culture medium. Cells were incubated for 0 or 48 h. Cell fractions were analyzed by NMR, then subsequently with MVDA. Differences in treatment provided detectable variations in the time of metabolic patterns of cell contents. Results indicated that glucose metabolism linked to glutamate was of major importance in the effects of inulin and its metabolites on Caco-2 cells under the conditions of our study. Metabolic fingerprinting in combination with an in vitro model appears to be a feasible technique with which to visualize metabolic patterns of cell contents and provides an efficient place for the generation of hypotheses about the metabolic pathways involved. In vitro metabolic fingerprinting may be of great benefit in the future for a better understanding of the relationship between nutrition and health.
Subject
Nutrition Packaging
Analytical research
1H NMR spectroscopy
Metabolic fingerprinting
Biochemical marker
Cell culture
Cell metabolism
Cell strain CACO 2
Chemical analysis
Controlled study
Experimental model
Glucose metabolism
Health status
Human cell
In vitro study
In vivo study
Laboratory test
Multivariate analysis
Nutrition
Pilot study
Proton nuclear magnetic resonance
Alanine
Analysis of Variance
Caco-2 Cells
Colon
Fermentation
Glucose
Glutamic Acid
Humans
Inulin
Ketoglutaric Acids
Lactic Acid
Magnetic Resonance Spectroscopy
Niacin
Niacinamide
Proline
Succinic Acid
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TNO identifier
237305
ISSN
0022-3166
Source
Journal of Nutrition, 133 (10), 3080-3084
Document type
article